Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds

The effect of homogenization in breaking protein-carotenoid complexes from cassava leaves was examined by monitoring three process conditions; amount of solvent per 0.5 g sample (ml), homogenization speed (rpm) and homogenization time (s). Acetone-n-hexane (1:1, v/v), tetrahydrofuran-methanol (1:...

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Main Authors: Jamal, Parveen, Jaswir, Irwandi, Hashim, Nurhasri Mulyadi, Azmi, Saiful Mohammad Nizam
Format: Book Chapter
Language:English
Published: IIUM Press 2011
Subjects:
Online Access:http://irep.iium.edu.my/20992/
http://irep.iium.edu.my/20992/1/effect_of_homogenization_-_C36.pdf
id iium-20992
recordtype eprints
spelling iium-209922012-07-05T04:13:05Z http://irep.iium.edu.my/20992/ Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds Jamal, Parveen Jaswir, Irwandi Hashim, Nurhasri Mulyadi Azmi, Saiful Mohammad Nizam TP248.13 Biotechnology The effect of homogenization in breaking protein-carotenoid complexes from cassava leaves was examined by monitoring three process conditions; amount of solvent per 0.5 g sample (ml), homogenization speed (rpm) and homogenization time (s). Acetone-n-hexane (1:1, v/v), tetrahydrofuran-methanol (1:1 v/v) and acetone were employed in the extraction process. The solvent that exhibited the best characteristics in extracting the highest b-carotene yield from the sample was selected for the optimizatIOn of process conditions for maximum b-carotene concentration, utilizing Face Centered Central Composite Design (FCCD) from Design Expert software, version 6 (DX6). A total of 20 experiments comprised of three factors, three levels and 6 center points were conducted to facilitate the optimization process. The maximum b-carotene yield of 17,544 flg/lOO g sample was achieved with the solvent ratio of 12 ml to 0.5 g sample, homogenization speed of 22000 rpm and homogemzation time of 57 s. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20992/1/effect_of_homogenization_-_C36.pdf Jamal, Parveen and Jaswir, Irwandi and Hashim, Nurhasri Mulyadi and Azmi, Saiful Mohammad Nizam (2011) Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 285-295. ISBN 9789674181444
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Jamal, Parveen
Jaswir, Irwandi
Hashim, Nurhasri Mulyadi
Azmi, Saiful Mohammad Nizam
Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
description The effect of homogenization in breaking protein-carotenoid complexes from cassava leaves was examined by monitoring three process conditions; amount of solvent per 0.5 g sample (ml), homogenization speed (rpm) and homogenization time (s). Acetone-n-hexane (1:1, v/v), tetrahydrofuran-methanol (1:1 v/v) and acetone were employed in the extraction process. The solvent that exhibited the best characteristics in extracting the highest b-carotene yield from the sample was selected for the optimizatIOn of process conditions for maximum b-carotene concentration, utilizing Face Centered Central Composite Design (FCCD) from Design Expert software, version 6 (DX6). A total of 20 experiments comprised of three factors, three levels and 6 center points were conducted to facilitate the optimization process. The maximum b-carotene yield of 17,544 flg/lOO g sample was achieved with the solvent ratio of 12 ml to 0.5 g sample, homogenization speed of 22000 rpm and homogemzation time of 57 s.
format Book Chapter
author Jamal, Parveen
Jaswir, Irwandi
Hashim, Nurhasri Mulyadi
Azmi, Saiful Mohammad Nizam
author_facet Jamal, Parveen
Jaswir, Irwandi
Hashim, Nurhasri Mulyadi
Azmi, Saiful Mohammad Nizam
author_sort Jamal, Parveen
title Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title_short Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title_full Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title_fullStr Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title_full_unstemmed Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title_sort effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
publisher IIUM Press
publishDate 2011
url http://irep.iium.edu.my/20992/
http://irep.iium.edu.my/20992/1/effect_of_homogenization_-_C36.pdf
first_indexed 2023-09-18T20:31:45Z
last_indexed 2023-09-18T20:31:45Z
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