Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
The effect of homogenization in breaking protein-carotenoid complexes from cassava leaves was examined by monitoring three process conditions; amount of solvent per 0.5 g sample (ml), homogenization speed (rpm) and homogenization time (s). Acetone-n-hexane (1:1, v/v), tetrahydrofuran-methanol (1:...
| Main Authors: | , , , |
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| Format: | Book Chapter |
| Language: | English |
| Published: |
IIUM Press
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/20992/ http://irep.iium.edu.my/20992/1/effect_of_homogenization_-_C36.pdf |
| Summary: | The effect of homogenization in breaking protein-carotenoid complexes from cassava leaves
was examined by monitoring three process conditions; amount of solvent per 0.5 g sample
(ml), homogenization speed (rpm) and homogenization time (s). Acetone-n-hexane (1:1, v/v),
tetrahydrofuran-methanol (1:1 v/v) and acetone were employed in the extraction process. The
solvent that exhibited the best characteristics in extracting the highest b-carotene yield from
the sample was selected for the optimizatIOn of process conditions for maximum b-carotene
concentration, utilizing Face Centered Central Composite Design (FCCD) from Design
Expert software, version 6 (DX6). A total of 20 experiments comprised of three factors, three
levels and 6 center points were conducted to facilitate the optimization process. The
maximum b-carotene yield of 17,544 flg/lOO g sample was achieved with the solvent ratio of
12 ml to 0.5 g sample, homogenization speed of 22000 rpm and homogemzation time of 57 s. |
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