A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates

A no\'el phyrale-degrnding enzyme namely PhyFAUIA I was purificd from a bacTerium i~lated from Malaysian wastewatcr. It is a pcriplasmaTic cnzymc and highly substrate-specific to phylaTe. The bacTerial cells were immobilized in alginate gel beads and iTS condition for preparaTion of myo-inosit...

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Main Authors:	Mohamad Puad, Noor Illi, Farouk, Abd Alaziem, Mohd. Salleh, Hamzah
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2011
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/20702/ http://irep.iium.edu.my/20702/ http://irep.iium.edu.my/20702/1/A_STUDY_OF_BACTERIAL_CELL_IMMOBILIZATION_IN_ALGINATE_GEL_BEADS_FOR_THE_PRODUCTION_OF_MYOINOSITOL_PHOSPHATES.pdf

id	iium-20702
recordtype	eprints
spelling	iium-207022012-11-27T07:29:14Z http://irep.iium.edu.my/20702/ A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates Mohamad Puad, Noor Illi Farouk, Abd Alaziem Mohd. Salleh, Hamzah TP Chemical technology TP248.13 Biotechnology A no\'el phyrale-degrnding enzyme namely PhyFAUIA I was purificd from a bacTerium i~lated from Malaysian wastewatcr. It is a pcriplasmaTic cnzymc and highly substrate-specific to phylaTe. The bacTerial cells were immobilized in alginate gel beads and iTS condition for preparaTion of myo-inositol phosphatcs was invcsligaled. The conditions for immobilizcd eclls were opTimized using dilTerem mcdia, residence Time and Temperature. Upon immob,lizalion. beads containing cells were sTored in groWlh medium at 4°C and Thc yicld can be reTained for more than 24 hours. The mechanism of reaction beTween phylaTe and PhyFAUIAI using immobilizcd cells was also invcsTigated and thcre were 2 possible paThways: (II PhyFAUlAl was seereted from the cells and went OUI from the beads to react Wilh phylatc. (2) PhyTate diffused Through lhc pores of alginate beads and reacTed WiTh phytasc locatcd inside the beads as well as aT The pcriplasmic OfThc cells. P, and IP were Then released from the beads. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20702/1/A_STUDY_OF_BACTERIAL_CELL_IMMOBILIZATION_IN_ALGINATE_GEL_BEADS_FOR_THE_PRODUCTION_OF_MYOINOSITOL_PHOSPHATES.pdf Mohamad Puad, Noor Illi and Farouk, Abd Alaziem and Mohd. Salleh, Hamzah (2011) A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates. In: Current Research and Development in Biotechnology Engineering at IIUM (volume II). IIUM Press, Kuala Lumpur, pp. 36-42. ISBN 978-967-418-151-2 http://rms.research.iium.edu.my/bookstore/default.aspx
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Mohamad Puad, Noor Illi Farouk, Abd Alaziem Mohd. Salleh, Hamzah A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates
description	A no\'el phyrale-degrnding enzyme namely PhyFAUIA I was purificd from a bacTerium i~lated from Malaysian wastewatcr. It is a pcriplasmaTic cnzymc and highly substrate-specific to phylaTe. The bacTerial cells were immobilized in alginate gel beads and iTS condition for preparaTion of myo-inositol phosphatcs was invcsligaled. The conditions for immobilizcd eclls were opTimized using dilTerem mcdia, residence Time and Temperature. Upon immob,lizalion. beads containing cells were sTored in groWlh medium at 4°C and Thc yicld can be reTained for more than 24 hours. The mechanism of reaction beTween phylaTe and PhyFAUIAI using immobilizcd cells was also invcsTigated and thcre were 2 possible paThways: (II PhyFAUlAl was seereted from the cells and went OUI from the beads to react Wilh phylatc. (2) PhyTate diffused Through lhc pores of alginate beads and reacTed WiTh phytasc locatcd inside the beads as well as aT The pcriplasmic OfThc cells. P, and IP were Then released from the beads.
format	Book Chapter
author	Mohamad Puad, Noor Illi Farouk, Abd Alaziem Mohd. Salleh, Hamzah
author_facet	Mohamad Puad, Noor Illi Farouk, Abd Alaziem Mohd. Salleh, Hamzah
author_sort	Mohamad Puad, Noor Illi
title	A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates
title_short	A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates
title_full	A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates
title_fullStr	A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates
title_full_unstemmed	A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates
title_sort	study of bacterial cell immobilization in alginate gel beads for the production of myo-inositol phosphates
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/20702/ http://irep.iium.edu.my/20702/ http://irep.iium.edu.my/20702/1/A_STUDY_OF_BACTERIAL_CELL_IMMOBILIZATION_IN_ALGINATE_GEL_BEADS_FOR_THE_PRODUCTION_OF_MYOINOSITOL_PHOSPHATES.pdf
first_indexed	2023-09-18T20:31:13Z
last_indexed	2023-09-18T20:31:13Z
_version_	1777408748891930624

A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates

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