Xanthine Oxidase inhibitory activity from potential Malaysian medicinal plants as remedies for gout

Malaysia has a rich diversity of medicinal plants and some of them have the property to inhibit xanthine oxidase (XO), which can be introduced as new natural source of gout medication and a substitute for synthetic xanthine oxidase inhibitors (XOI), specifically Allopurinol which has many side effec...

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Main Authors:	Azmi, Saiful Mohammad Nizam, Jamal, Parveen, Amid, Azura
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	RM300 Drugs and their action TA164 Bioengineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/16827/ http://irep.iium.edu.my/16827/ http://irep.iium.edu.my/16827/1/ICFR_2010_XOI.pdf

id	iium-16827
recordtype	eprints
spelling	iium-168272012-01-27T03:20:16Z http://irep.iium.edu.my/16827/ Xanthine Oxidase inhibitory activity from potential Malaysian medicinal plants as remedies for gout Azmi, Saiful Mohammad Nizam Jamal, Parveen Amid, Azura RM300 Drugs and their action TA164 Bioengineering TP248.13 Biotechnology Malaysia has a rich diversity of medicinal plants and some of them have the property to inhibit xanthine oxidase (XO), which can be introduced as new natural source of gout medication and a substitute for synthetic xanthine oxidase inhibitors (XOI), specifically Allopurinol which has many side effects. Fifty samples from twenty plant species were examined for XO inhibitory activity. Selection of plants was based on their frequent usages by local folks for their medicinal benefits and activities in three different extraction solvents; distilled water, 70% methanol, 100% ethanol. The degree of XO inhibition was determined by measuring the absorbance spectrophotometrically at 295 nm associated with uric acid formation. Ninety-nine percent of the crude extracts were found to have XO inhibitory activity at 100 μg/ml, and 57% showed greater than 50% inhibition. On the basis of availability and sustainability, distilled water extract of Carica papaya leaves was selected for optimization of process conditions, namely, extraction temperature (⁰C), extraction time (hour), agitation speed (rpm), and ratio of sample to the solvent (1 g/ml) using Central Composite Design (CCD) of Response Surface Methodology (RSM) from Design Expert© v.6.0.8 software. The analysis revealed that the maximum %XOI activity can be reached with these conditions: 30⁰C, 17 hour, 125 rpm and 1g/19ml. The optimized conditions were verified and the maximum %XOI obtained was 86.93%, lower 7% yet comparable to the activity exhibited by Allopurinol but safer and less cost incurred. 2010-11 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/16827/1/ICFR_2010_XOI.pdf Azmi, Saiful Mohammad Nizam and Jamal, Parveen and Amid, Azura (2010) Xanthine Oxidase inhibitory activity from potential Malaysian medicinal plants as remedies for gout. In: International Conference on Food Research (ICFR 2010), 22 - 24 November 2010, Marriot Putrajaya, Malaysia. http://www.icf2010.upm.edu.my
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM300 Drugs and their action TA164 Bioengineering TP248.13 Biotechnology
spellingShingle	RM300 Drugs and their action TA164 Bioengineering TP248.13 Biotechnology Azmi, Saiful Mohammad Nizam Jamal, Parveen Amid, Azura Xanthine Oxidase inhibitory activity from potential Malaysian medicinal plants as remedies for gout
description	Malaysia has a rich diversity of medicinal plants and some of them have the property to inhibit xanthine oxidase (XO), which can be introduced as new natural source of gout medication and a substitute for synthetic xanthine oxidase inhibitors (XOI), specifically Allopurinol which has many side effects. Fifty samples from twenty plant species were examined for XO inhibitory activity. Selection of plants was based on their frequent usages by local folks for their medicinal benefits and activities in three different extraction solvents; distilled water, 70% methanol, 100% ethanol. The degree of XO inhibition was determined by measuring the absorbance spectrophotometrically at 295 nm associated with uric acid formation. Ninety-nine percent of the crude extracts were found to have XO inhibitory activity at 100 μg/ml, and 57% showed greater than 50% inhibition. On the basis of availability and sustainability, distilled water extract of Carica papaya leaves was selected for optimization of process conditions, namely, extraction temperature (⁰C), extraction time (hour), agitation speed (rpm), and ratio of sample to the solvent (1 g/ml) using Central Composite Design (CCD) of Response Surface Methodology (RSM) from Design Expert© v.6.0.8 software. The analysis revealed that the maximum %XOI activity can be reached with these conditions: 30⁰C, 17 hour, 125 rpm and 1g/19ml. The optimized conditions were verified and the maximum %XOI obtained was 86.93%, lower 7% yet comparable to the activity exhibited by Allopurinol but safer and less cost incurred.
format	Conference or Workshop Item
author	Azmi, Saiful Mohammad Nizam Jamal, Parveen Amid, Azura
author_facet	Azmi, Saiful Mohammad Nizam Jamal, Parveen Amid, Azura
author_sort	Azmi, Saiful Mohammad Nizam
title	Xanthine Oxidase inhibitory activity from potential Malaysian medicinal plants as remedies for gout
title_short	Xanthine Oxidase inhibitory activity from potential Malaysian medicinal plants as remedies for gout
title_full	Xanthine Oxidase inhibitory activity from potential Malaysian medicinal plants as remedies for gout
title_fullStr	Xanthine Oxidase inhibitory activity from potential Malaysian medicinal plants as remedies for gout
title_full_unstemmed	Xanthine Oxidase inhibitory activity from potential Malaysian medicinal plants as remedies for gout
title_sort	xanthine oxidase inhibitory activity from potential malaysian medicinal plants as remedies for gout
publishDate	2010
url	http://irep.iium.edu.my/16827/ http://irep.iium.edu.my/16827/ http://irep.iium.edu.my/16827/1/ICFR_2010_XOI.pdf
first_indexed	2023-09-18T20:25:33Z
last_indexed	2023-09-18T20:25:33Z
_version_	1777408392082489344

Xanthine Oxidase inhibitory activity from potential Malaysian medicinal plants as remedies for gout

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