Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient

Hepatitis C is a never-ending public health problem and the prevalence among chronic renal disease (CRD) patient always exceeds the general population. We studied the sensitivity of semi-nested reverse-transcription polymerase chain reaction (sn RT-PCR) technique for the detection of hepatitis C vir...

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Main Authors:	Hamzah, Hairul Aini, Mustafa Mahmoud, Mohammed Imad Al-Deen, Abdullah , Nasuruddin
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	QR Microbiology QR355 Virology
Online Access:	http://irep.iium.edu.my/13317/ http://irep.iium.edu.my/13317/ http://irep.iium.edu.my/13317/2/IHCI_2011_sensitivity_assay.pdf

id	iium-13317
recordtype	eprints
spelling	iium-133172017-06-15T00:48:04Z http://irep.iium.edu.my/13317/ Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah , Nasuruddin QR Microbiology QR355 Virology Hepatitis C is a never-ending public health problem and the prevalence among chronic renal disease (CRD) patient always exceeds the general population. We studied the sensitivity of semi-nested reverse-transcription polymerase chain reaction (sn RT-PCR) technique for the detection of hepatitis C virus (HCV) infection among patients with chronic renal problem who underwent haemodialysis treatment. An assay was evaluated using a positive control with known HCV viral load (2,148,000 IU/ml), which was determined at Gribbles Laboratory (Australia). A 10-fold serial dilution of the serum sample was conducted prior to RNA extraction and sn RT-PCR. The amplicon band was observed by electrophoresis in 1.7 % gel. Then, fifty-three (53) patients with known no infection to the HCV were collected and further tested with the established assay. The sensitivity result showed that the assay was sensitive enough to detect viral RNA up to 1:10 000 dilution (2.148 IU/ml). Further investigation on the serum samples of the CRD patients revealed four positive cases (7.6 %) which one of them seroconverted to positive. In conclusion, the established assay was highly sensitive in detecting HCV infection among seronegative CRD patient. The assay can be further improved and used as a HCV screening tool. 2011-12-07 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/13317/2/IHCI_2011_sensitivity_assay.pdf Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and Abdullah , Nasuruddin (2011) Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient. In: International Health Conference IIUM (IHCI) 2011 , 7-8 Dec 2011, Vistana Hotel Kuantan. http://www.iium.edu.my/ihsci2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology QR355 Virology
spellingShingle	QR Microbiology QR355 Virology Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah , Nasuruddin Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient
description	Hepatitis C is a never-ending public health problem and the prevalence among chronic renal disease (CRD) patient always exceeds the general population. We studied the sensitivity of semi-nested reverse-transcription polymerase chain reaction (sn RT-PCR) technique for the detection of hepatitis C virus (HCV) infection among patients with chronic renal problem who underwent haemodialysis treatment. An assay was evaluated using a positive control with known HCV viral load (2,148,000 IU/ml), which was determined at Gribbles Laboratory (Australia). A 10-fold serial dilution of the serum sample was conducted prior to RNA extraction and sn RT-PCR. The amplicon band was observed by electrophoresis in 1.7 % gel. Then, fifty-three (53) patients with known no infection to the HCV were collected and further tested with the established assay. The sensitivity result showed that the assay was sensitive enough to detect viral RNA up to 1:10 000 dilution (2.148 IU/ml). Further investigation on the serum samples of the CRD patients revealed four positive cases (7.6 %) which one of them seroconverted to positive. In conclusion, the established assay was highly sensitive in detecting HCV infection among seronegative CRD patient. The assay can be further improved and used as a HCV screening tool.
format	Conference or Workshop Item
author	Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah , Nasuruddin
author_facet	Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah , Nasuruddin
author_sort	Hamzah, Hairul Aini
title	Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient
title_short	Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient
title_full	Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient
title_fullStr	Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient
title_full_unstemmed	Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient
title_sort	sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting hcv-rna from chronic renal disease patient
publishDate	2011
url	http://irep.iium.edu.my/13317/ http://irep.iium.edu.my/13317/ http://irep.iium.edu.my/13317/2/IHCI_2011_sensitivity_assay.pdf
first_indexed	2023-09-18T20:22:30Z
last_indexed	2023-09-18T20:22:30Z
_version_	1777408200507654144

Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient

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