Rnase Purification from Isolate Rns

Bacteria are the most preferred sources of RNase due to their broad biochemical diversity. The present study involved oOptimization and partial purification of RNase from a bacterial isolate from soil sample. Out of 12 bacterial isolates one isolate (RNS 3) was selected based on relatively higher RN...

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Main Authors:	Puranjan, Mishra, Singh, Lakhveer, Zularisam, A. W.
Format:	Article
Language:	English
Published:	Penerbit UMP 2014
Subjects:	T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/9524/ http://umpir.ump.edu.my/id/eprint/9524/ http://umpir.ump.edu.my/id/eprint/9524/1/6.pdf

id	ump-9524
recordtype	eprints
spelling	ump-95242019-09-10T02:42:01Z http://umpir.ump.edu.my/id/eprint/9524/ Rnase Purification from Isolate Rns Puranjan, Mishra Singh, Lakhveer Zularisam, A. W. T Technology (General) Bacteria are the most preferred sources of RNase due to their broad biochemical diversity. The present study involved oOptimization and partial purification of RNase from a bacterial isolate from soil sample. Out of 12 bacterial isolates one isolate (RNS 3) was selected based on relatively higher RNase activity in the culture broth. The various physico-chemical parameters wereare consecutively optimized for maximum enzyme production. The optimized parameters are at were temperature of 34°C, incubation period (20 h) and inoculum size (10% v/v). Among different carbon and nitrogen sources supplementation of glucose and peptone, respectively to the broth showed enhanced RNase production. An alkaline pH (8.5) was suitable for maximum enzyme production by the bacterial isolate RNS3. Penerbit UMP 2014-06 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/9524/1/6.pdf Puranjan, Mishra and Singh, Lakhveer and Zularisam, A. W. (2014) Rnase Purification from Isolate Rns. International Journal of Engineering Technology & Sciences, 1 (1). pp. 2035-2037. ISSN 2289-697X http://ijets.ump.edu.my/images/archive/Vol1/6.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Puranjan, Mishra Singh, Lakhveer Zularisam, A. W. Rnase Purification from Isolate Rns
description	Bacteria are the most preferred sources of RNase due to their broad biochemical diversity. The present study involved oOptimization and partial purification of RNase from a bacterial isolate from soil sample. Out of 12 bacterial isolates one isolate (RNS 3) was selected based on relatively higher RNase activity in the culture broth. The various physico-chemical parameters wereare consecutively optimized for maximum enzyme production. The optimized parameters are at were temperature of 34°C, incubation period (20 h) and inoculum size (10% v/v). Among different carbon and nitrogen sources supplementation of glucose and peptone, respectively to the broth showed enhanced RNase production. An alkaline pH (8.5) was suitable for maximum enzyme production by the bacterial isolate RNS3.
format	Article
author	Puranjan, Mishra Singh, Lakhveer Zularisam, A. W.
author_facet	Puranjan, Mishra Singh, Lakhveer Zularisam, A. W.
author_sort	Puranjan, Mishra
title	Rnase Purification from Isolate Rns
title_short	Rnase Purification from Isolate Rns
title_full	Rnase Purification from Isolate Rns
title_fullStr	Rnase Purification from Isolate Rns
title_full_unstemmed	Rnase Purification from Isolate Rns
title_sort	rnase purification from isolate rns
publisher	Penerbit UMP
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/9524/ http://umpir.ump.edu.my/id/eprint/9524/ http://umpir.ump.edu.my/id/eprint/9524/1/6.pdf
first_indexed	2023-09-18T22:08:11Z
last_indexed	2023-09-18T22:08:11Z
_version_	1777414848970227712

Rnase Purification from Isolate Rns

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