Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability

The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification and more favorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes...

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Main Authors: Rohaida, Che Man, Ahmad Fauzi, Ismail, Nazlee Faisal, Ghazali, Siti Fatimah Zaharah, Mohd Fuzi, Rosli, Md. Illias
Format: Article
Language:English
Published: Elsevier 2015
Subjects:
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http://umpir.ump.edu.my/id/eprint/9404/1/fkksa-2015-rohaida-Effects%20of%20the%20Immobilization.pdf
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spelling ump-94042017-10-30T01:58:54Z http://umpir.ump.edu.my/id/eprint/9404/ Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability Rohaida, Che Man Ahmad Fauzi, Ismail Nazlee Faisal, Ghazali Siti Fatimah Zaharah, Mohd Fuzi Rosli, Md. Illias TP Chemical technology The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification and more favorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes when using Escherichia coli as a host. In this study, the effects of different polymer of hollow fiber membrane and culture conditions on the enzyme excretion, cell lysis and plasmid stability of immobilized E. coli were investigated. The cells immobilized on a hollow fiber membrane composed of a polyvinylidene fluoride (PVDF) polymer exhibited a 2–4-fold increase in CGTase excretion, over a 100% increase in plasmid stability and 28-60% reduction in cell lysis compared with free cells. Hence, the immobilization of E. coli using a hollow fiber membrane was demonstrated to increase enzyme excretion and cell stability. Elsevier 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9404/1/fkksa-2015-rohaida-Effects%20of%20the%20Immobilization.pdf Rohaida, Che Man and Ahmad Fauzi, Ismail and Nazlee Faisal, Ghazali and Siti Fatimah Zaharah, Mohd Fuzi and Rosli, Md. Illias (2015) Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability. Biochemical Engineering Journal, 98. pp. 91-98. ISSN 1369-703X http://dx.doi.org/10.1016/j.bej.2015.02.013 doi: 10.1016/j.bej.2015.02.013
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Rohaida, Che Man
Ahmad Fauzi, Ismail
Nazlee Faisal, Ghazali
Siti Fatimah Zaharah, Mohd Fuzi
Rosli, Md. Illias
Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
description The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification and more favorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes when using Escherichia coli as a host. In this study, the effects of different polymer of hollow fiber membrane and culture conditions on the enzyme excretion, cell lysis and plasmid stability of immobilized E. coli were investigated. The cells immobilized on a hollow fiber membrane composed of a polyvinylidene fluoride (PVDF) polymer exhibited a 2–4-fold increase in CGTase excretion, over a 100% increase in plasmid stability and 28-60% reduction in cell lysis compared with free cells. Hence, the immobilization of E. coli using a hollow fiber membrane was demonstrated to increase enzyme excretion and cell stability.
format Article
author Rohaida, Che Man
Ahmad Fauzi, Ismail
Nazlee Faisal, Ghazali
Siti Fatimah Zaharah, Mohd Fuzi
Rosli, Md. Illias
author_facet Rohaida, Che Man
Ahmad Fauzi, Ismail
Nazlee Faisal, Ghazali
Siti Fatimah Zaharah, Mohd Fuzi
Rosli, Md. Illias
author_sort Rohaida, Che Man
title Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
title_short Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
title_full Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
title_fullStr Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
title_full_unstemmed Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
title_sort effects of the immobilization of recombinant escherichia coli on cyclodextrin glucanotransferase (cgtase) excretion and cell viability
publisher Elsevier
publishDate 2015
url http://umpir.ump.edu.my/id/eprint/9404/
http://umpir.ump.edu.my/id/eprint/9404/
http://umpir.ump.edu.my/id/eprint/9404/
http://umpir.ump.edu.my/id/eprint/9404/1/fkksa-2015-rohaida-Effects%20of%20the%20Immobilization.pdf
first_indexed 2023-09-18T22:07:57Z
last_indexed 2023-09-18T22:07:57Z
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