Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability

The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification and more favorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes...

Full description

Bibliographic Details
Main Authors:	Rohaida, Che Man, Ahmad Fauzi, Ismail, Nazlee Faisal, Ghazali, Siti Fatimah Zaharah, Mohd Fuzi, Rosli, Md. Illias
Format:	Article
Language:	English
Published:	Elsevier 2015
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/9404/ http://umpir.ump.edu.my/id/eprint/9404/ http://umpir.ump.edu.my/id/eprint/9404/ http://umpir.ump.edu.my/id/eprint/9404/1/fkksa-2015-rohaida-Effects%20of%20the%20Immobilization.pdf

id	ump-9404
recordtype	eprints
spelling	ump-94042017-10-30T01:58:54Z http://umpir.ump.edu.my/id/eprint/9404/ Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability Rohaida, Che Man Ahmad Fauzi, Ismail Nazlee Faisal, Ghazali Siti Fatimah Zaharah, Mohd Fuzi Rosli, Md. Illias TP Chemical technology The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification and more favorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes when using Escherichia coli as a host. In this study, the effects of different polymer of hollow fiber membrane and culture conditions on the enzyme excretion, cell lysis and plasmid stability of immobilized E. coli were investigated. The cells immobilized on a hollow fiber membrane composed of a polyvinylidene fluoride (PVDF) polymer exhibited a 2–4-fold increase in CGTase excretion, over a 100% increase in plasmid stability and 28-60% reduction in cell lysis compared with free cells. Hence, the immobilization of E. coli using a hollow fiber membrane was demonstrated to increase enzyme excretion and cell stability. Elsevier 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9404/1/fkksa-2015-rohaida-Effects%20of%20the%20Immobilization.pdf Rohaida, Che Man and Ahmad Fauzi, Ismail and Nazlee Faisal, Ghazali and Siti Fatimah Zaharah, Mohd Fuzi and Rosli, Md. Illias (2015) Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability. Biochemical Engineering Journal, 98. pp. 91-98. ISSN 1369-703X http://dx.doi.org/10.1016/j.bej.2015.02.013 doi: 10.1016/j.bej.2015.02.013
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Rohaida, Che Man Ahmad Fauzi, Ismail Nazlee Faisal, Ghazali Siti Fatimah Zaharah, Mohd Fuzi Rosli, Md. Illias Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
description	The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification and more favorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes when using Escherichia coli as a host. In this study, the effects of different polymer of hollow fiber membrane and culture conditions on the enzyme excretion, cell lysis and plasmid stability of immobilized E. coli were investigated. The cells immobilized on a hollow fiber membrane composed of a polyvinylidene fluoride (PVDF) polymer exhibited a 2–4-fold increase in CGTase excretion, over a 100% increase in plasmid stability and 28-60% reduction in cell lysis compared with free cells. Hence, the immobilization of E. coli using a hollow fiber membrane was demonstrated to increase enzyme excretion and cell stability.
format	Article
author	Rohaida, Che Man Ahmad Fauzi, Ismail Nazlee Faisal, Ghazali Siti Fatimah Zaharah, Mohd Fuzi Rosli, Md. Illias
author_facet	Rohaida, Che Man Ahmad Fauzi, Ismail Nazlee Faisal, Ghazali Siti Fatimah Zaharah, Mohd Fuzi Rosli, Md. Illias
author_sort	Rohaida, Che Man
title	Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
title_short	Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
title_full	Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
title_fullStr	Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
title_full_unstemmed	Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
title_sort	effects of the immobilization of recombinant escherichia coli on cyclodextrin glucanotransferase (cgtase) excretion and cell viability
publisher	Elsevier
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/9404/ http://umpir.ump.edu.my/id/eprint/9404/ http://umpir.ump.edu.my/id/eprint/9404/ http://umpir.ump.edu.my/id/eprint/9404/1/fkksa-2015-rohaida-Effects%20of%20the%20Immobilization.pdf
first_indexed	2023-09-18T22:07:57Z
last_indexed	2023-09-18T22:07:57Z
_version_	1777414834513510400

Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability

Similar Items