Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface

This thesis presents the effect of the bacterial adhesion on the glass surface (hydrophilic surfaces) at different time exposure and bacterial concentration. The ability of Escherichia coli and Bacillus subtilis to attach to the surfaces depends mainly on the interaction of hydrophobic domains. Howe...

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Main Author:	Nasuha, Ibrahim
Format:	Undergraduates Project Papers
Language:	English
Published:	2014
Subjects:	QC Physics
Online Access:	http://umpir.ump.edu.my/id/eprint/9209/ http://umpir.ump.edu.my/id/eprint/9209/ http://umpir.ump.edu.my/id/eprint/9209/1/cd8610.pdf

id	ump-9209
recordtype	eprints
spelling	ump-92092015-10-13T00:10:32Z http://umpir.ump.edu.my/id/eprint/9209/ Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface Nasuha, Ibrahim QC Physics This thesis presents the effect of the bacterial adhesion on the glass surface (hydrophilic surfaces) at different time exposure and bacterial concentration. The ability of Escherichia coli and Bacillus subtilis to attach to the surfaces depends mainly on the interaction of hydrophobic domains. However, E. coli and B. subtilis have evolved in different ways in order to manipulate the hydrophobic effect for their adherence on the solid surface. On the other hand, the surface properties e.g surface charges are inherently important and often regulate the mechanism of the bacteria adhesion. Besides that, adhesions of bacteria were also affected by culture media, exposure time of bacteria on glass surface, age and bacterial concentration. Both bacteria have different surface characteristic which also affect adhesion on the glass surface. Both bacteria were suspended in the phosphate buffer solution (pH 7.1) at different cell concentration (abs). The solution was suspended into glass container containing glass slide. The glass-bacterial solution was shake at 100 rpm and 30⁰C in the incubator shaker and sampling were done at 4 h, 8 h, 12 h and 24 h. From the researches that have been done B. subtilis easily adhere on the glass surface compared to E. coli, with 46.9% reduction in optical density reading observed at 600nm. Bacillus subtilis was exposed for 24 hour at cell concentration 0.8 abs. Meanwhile, E. coli result in less adhesion to the glass surface with only 29.8 % reduction in optical density. Yet, the time of exposure for E. coli was only 12 hour with cell concentration 1.0 abs 2014-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9209/1/cd8610.pdf Nasuha, Ibrahim (2014) Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:84641&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QC Physics
spellingShingle	QC Physics Nasuha, Ibrahim Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface
description	This thesis presents the effect of the bacterial adhesion on the glass surface (hydrophilic surfaces) at different time exposure and bacterial concentration. The ability of Escherichia coli and Bacillus subtilis to attach to the surfaces depends mainly on the interaction of hydrophobic domains. However, E. coli and B. subtilis have evolved in different ways in order to manipulate the hydrophobic effect for their adherence on the solid surface. On the other hand, the surface properties e.g surface charges are inherently important and often regulate the mechanism of the bacteria adhesion. Besides that, adhesions of bacteria were also affected by culture media, exposure time of bacteria on glass surface, age and bacterial concentration. Both bacteria have different surface characteristic which also affect adhesion on the glass surface. Both bacteria were suspended in the phosphate buffer solution (pH 7.1) at different cell concentration (abs). The solution was suspended into glass container containing glass slide. The glass-bacterial solution was shake at 100 rpm and 30⁰C in the incubator shaker and sampling were done at 4 h, 8 h, 12 h and 24 h. From the researches that have been done B. subtilis easily adhere on the glass surface compared to E. coli, with 46.9% reduction in optical density reading observed at 600nm. Bacillus subtilis was exposed for 24 hour at cell concentration 0.8 abs. Meanwhile, E. coli result in less adhesion to the glass surface with only 29.8 % reduction in optical density. Yet, the time of exposure for E. coli was only 12 hour with cell concentration 1.0 abs
format	Undergraduates Project Papers
author	Nasuha, Ibrahim
author_facet	Nasuha, Ibrahim
author_sort	Nasuha, Ibrahim
title	Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface
title_short	Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface
title_full	Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface
title_fullStr	Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface
title_full_unstemmed	Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface
title_sort	effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/9209/ http://umpir.ump.edu.my/id/eprint/9209/ http://umpir.ump.edu.my/id/eprint/9209/1/cd8610.pdf
first_indexed	2023-09-18T22:07:33Z
last_indexed	2023-09-18T22:07:33Z
_version_	1777414809168379904

Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface

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