Potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer

This paper presents antibacterial and antifungal study of papain enzyme and its potential in making hand wash and hand sanitizer. Papain enzyme could be obtained from papaya latex, fruits and leaves. In this research, papaya leaves chose for the papain extraction because it is biomass and easily to...

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Main Author:	Nguang, Suok Ling
Format:	Undergraduates Project Papers
Language:	English
Published:	2013
Subjects:	QP Physiology
Online Access:	http://umpir.ump.edu.my/id/eprint/9098/ http://umpir.ump.edu.my/id/eprint/9098/ http://umpir.ump.edu.my/id/eprint/9098/1/CD8570%20%40%2084.pdf

id	ump-9098
recordtype	eprints
spelling	ump-90982015-10-21T00:07:59Z http://umpir.ump.edu.my/id/eprint/9098/ Potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer Nguang, Suok Ling QP Physiology This paper presents antibacterial and antifungal study of papain enzyme and its potential in making hand wash and hand sanitizer. Papain enzyme could be obtained from papaya latex, fruits and leaves. In this research, papaya leaves chose for the papain extraction because it is biomass and easily to get the source. The extraction method used in this research is hot water extraction follow by the Folin & Ciocalteu papain analysis method. Before making the antibacterial and antifungal handwash and hand sanitizer, antibacterial and antifungal test was done in order to proof the antibacterial and antifungal properties of papain enzyme. Antibacterial and antifungal test were done by incubating the pathogens on the nutrient agar in incubator for three days then insert papain enzyme onto it then incubate for another three days to determine the mycelial growth of pathogens. A volume of 187mL of papain solution was extracted by 21.55g and 200mL water. During the papain enzyme analysis, dark blue solution formed when Folin & Ciocalteu reagent was added into the solution sample. The blue colour indicate the present of tpapain enzyme. Enzyme concentration was measured in 0.415μm while the enzyme activity is 0.2283unit/mL. It means that 10mL of the papain solution extracted contains 2.283 unit of papain enzyme. The mycelial growth of the Staphylococcus aureus, Escherichia coli and Bacillus subtilis bacteria and Saccharomyces cerevisiae, Rhizopus spp. and Mucor sppfungus were obviously decline 3 days after the papain solution added. Antibacterial and antifungal handwash and hand sanitizer were successfully produced at the end of this research 2013-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9098/1/CD8570%20%40%2084.pdf Nguang, Suok Ling (2013) Potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer. Faculty Of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:84690&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QP Physiology
spellingShingle	QP Physiology Nguang, Suok Ling Potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer
description	This paper presents antibacterial and antifungal study of papain enzyme and its potential in making hand wash and hand sanitizer. Papain enzyme could be obtained from papaya latex, fruits and leaves. In this research, papaya leaves chose for the papain extraction because it is biomass and easily to get the source. The extraction method used in this research is hot water extraction follow by the Folin & Ciocalteu papain analysis method. Before making the antibacterial and antifungal handwash and hand sanitizer, antibacterial and antifungal test was done in order to proof the antibacterial and antifungal properties of papain enzyme. Antibacterial and antifungal test were done by incubating the pathogens on the nutrient agar in incubator for three days then insert papain enzyme onto it then incubate for another three days to determine the mycelial growth of pathogens. A volume of 187mL of papain solution was extracted by 21.55g and 200mL water. During the papain enzyme analysis, dark blue solution formed when Folin & Ciocalteu reagent was added into the solution sample. The blue colour indicate the present of tpapain enzyme. Enzyme concentration was measured in 0.415μm while the enzyme activity is 0.2283unit/mL. It means that 10mL of the papain solution extracted contains 2.283 unit of papain enzyme. The mycelial growth of the Staphylococcus aureus, Escherichia coli and Bacillus subtilis bacteria and Saccharomyces cerevisiae, Rhizopus spp. and Mucor sppfungus were obviously decline 3 days after the papain solution added. Antibacterial and antifungal handwash and hand sanitizer were successfully produced at the end of this research
format	Undergraduates Project Papers
author	Nguang, Suok Ling
author_facet	Nguang, Suok Ling
author_sort	Nguang, Suok Ling
title	Potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer
title_short	Potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer
title_full	Potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer
title_fullStr	Potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer
title_full_unstemmed	Potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer
title_sort	potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/9098/ http://umpir.ump.edu.my/id/eprint/9098/ http://umpir.ump.edu.my/id/eprint/9098/1/CD8570%20%40%2084.pdf
first_indexed	2023-09-18T22:07:18Z
last_indexed	2023-09-18T22:07:18Z
_version_	1777414793899016192

Potential application of papain enzyme from papaya leaves in making antibacteria and antifungal handwash and hand sanitizer

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