Kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation

This research is about the kinetic study on poly(3-hydroxybutyrate), P(3HB) production from oil palm frond juice in batch fermentation. Batch fermentation experiment was conducted in 500 mL shake flasks by using Cupriavidus necator in order to produce P(3HB). Fresh oil palm frond (OPF) was pressed a...

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Main Author:	Nurul Amalina, Alias
Format:	Undergraduates Project Papers
Language:	English
Published:	2014
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/9090/ http://umpir.ump.edu.my/id/eprint/9090/ http://umpir.ump.edu.my/id/eprint/9090/1/CD8630%20%40%2048.pdf

id	ump-9090
recordtype	eprints
spelling	ump-90902015-10-21T00:08:08Z http://umpir.ump.edu.my/id/eprint/9090/ Kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation Nurul Amalina, Alias TP Chemical technology This research is about the kinetic study on poly(3-hydroxybutyrate), P(3HB) production from oil palm frond juice in batch fermentation. Batch fermentation experiment was conducted in 500 mL shake flasks by using Cupriavidus necator in order to produce P(3HB). Fresh oil palm frond (OPF) was pressed and undergoes certain processes such as filtration, centrifugation and sterilization to get oil palm frond juice. OPF juice were used as the fermentation substrate for P(3HB) production. In this study, the microbial growth (μ, μmax, YX/S, Ks) and product (YP/S, YP/X) kinetic parameter can be obtained by using MATLAB R2009b software. The models were fit to the data using Runge-Kutta 4th order Method by minimizing the error between experimental and predicted data using Simplex Method. Logistic Model was developed for the biomass growth model. Model for polyhydroxyalkanoates (PHA) was developed based on assumptions that each cell contains the same amount of PHA and it degrades at the same rate. Predicted data was obtained using function ode45 in MATLAB R2009b software which implements Runge-Kutta 4th Order Method. Based on the results obtained, it shows that the predicted data of biomass profile was fitted well to the experimental data but for PHB profile, the predicted data deviated quite far from the experimental data. This shown that the models developed were representing biomass growth very well. Kinetic parameters for microbial growth and production of P(3HB) were determined by using Monod equation. The values are = 0.0346h-1, Ks = 0.0930g/L, = 0.0344h-1, Yx/s= 0.4731 and = 0.0416 2014-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9090/1/CD8630%20%40%2048.pdf Nurul Amalina, Alias (2014) Kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation. Faculty Of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:84624&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nurul Amalina, Alias Kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation
description	This research is about the kinetic study on poly(3-hydroxybutyrate), P(3HB) production from oil palm frond juice in batch fermentation. Batch fermentation experiment was conducted in 500 mL shake flasks by using Cupriavidus necator in order to produce P(3HB). Fresh oil palm frond (OPF) was pressed and undergoes certain processes such as filtration, centrifugation and sterilization to get oil palm frond juice. OPF juice were used as the fermentation substrate for P(3HB) production. In this study, the microbial growth (μ, μmax, YX/S, Ks) and product (YP/S, YP/X) kinetic parameter can be obtained by using MATLAB R2009b software. The models were fit to the data using Runge-Kutta 4th order Method by minimizing the error between experimental and predicted data using Simplex Method. Logistic Model was developed for the biomass growth model. Model for polyhydroxyalkanoates (PHA) was developed based on assumptions that each cell contains the same amount of PHA and it degrades at the same rate. Predicted data was obtained using function ode45 in MATLAB R2009b software which implements Runge-Kutta 4th Order Method. Based on the results obtained, it shows that the predicted data of biomass profile was fitted well to the experimental data but for PHB profile, the predicted data deviated quite far from the experimental data. This shown that the models developed were representing biomass growth very well. Kinetic parameters for microbial growth and production of P(3HB) were determined by using Monod equation. The values are = 0.0346h-1, Ks = 0.0930g/L, = 0.0344h-1, Yx/s= 0.4731 and = 0.0416
format	Undergraduates Project Papers
author	Nurul Amalina, Alias
author_facet	Nurul Amalina, Alias
author_sort	Nurul Amalina, Alias
title	Kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation
title_short	Kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation
title_full	Kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation
title_fullStr	Kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation
title_full_unstemmed	Kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation
title_sort	kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/9090/ http://umpir.ump.edu.my/id/eprint/9090/ http://umpir.ump.edu.my/id/eprint/9090/1/CD8630%20%40%2048.pdf
first_indexed	2023-09-18T22:07:17Z
last_indexed	2023-09-18T22:07:17Z
_version_	1777414792742436864

Kinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation

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