Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties

In this present study, we reported broccoli (Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles ( NPs) in green chemistry method . The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organism...

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Main Authors:	Kuppusamy, Palaniselvam, M. M., Yusoff, Narasimha, Reddy Parine, Solachuddin J.A., Ichwan, Gaanty Pragas, Maniam, Natanamurugaraj, Govindan
Format:	Article
Language:	English
Published:	2015
Subjects:	TD Environmental technology. Sanitary engineering
Online Access:	http://umpir.ump.edu.my/id/eprint/8923/ http://umpir.ump.edu.my/id/eprint/8923/ http://umpir.ump.edu.my/id/eprint/8923/ http://umpir.ump.edu.my/id/eprint/8923/7/fist-2015-Natanamurugaraj-Intracellular%20Biosynthesis%20Of%20Au%20And%20Ag.pdf

id	ump-8923
recordtype	eprints
spelling	ump-89232018-07-27T01:33:43Z http://umpir.ump.edu.my/id/eprint/8923/ Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties Kuppusamy, Palaniselvam M. M., Yusoff Narasimha, Reddy Parine Solachuddin J.A., Ichwan Gaanty Pragas, Maniam Natanamurugaraj, Govindan TD Environmental technology. Sanitary engineering In this present study, we reported broccoli (Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles ( NPs) in green chemistry method . The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organisms. The physico-chemical properties of gold nanoparticles were analyzed using different analytical techniques such as a UV–Vis spectrophotometer , Field Emission Scanning Electron Micros copy, energy dispersive X-ray spectroscopy, X-ray diffraction and a Fourier Transform Infrared spectrophotometer. In addition, gold and silver NP antimicrobial efficacy was checked by disc diffusion assay. UV–Vis color intensity of the nanoparticles was shown at 540 and 450 nm for gold and silver nanoparticles respectively. Higher magnification of the Field Emission Scanning Electron Microscopy image shows the variable morphology of the gold nanoparticles such as spherical, rod and triangular shapes and silver nanop articles were seen in spherical shapes. The average spherical size of the particles was observed in 24– 38 nm for gold and 30–45 nm for silver NPs . X-ray diffraction pattern confirmed the presence of gold nanoparticles and silver nanoparticles which were crystalline in nature. Additionally , the functional metabolites were identified by the Fourier Transform Infrared spectroscopy. IR spectra revealed phenols, alcohols, aldehydes ( sugar moieties), vitamins and proteins are present in the broccoli extract which are accountable to synthesize the nanoparticles. The synthesized gold and silver NPs inhibited the growth of the tested bacterial and fungal pathogens at the concentration of 50 μg/mL respectively. Inaddition, broccoli mediated gold and silver nanoparticles have shown potent antimicrobial activity against human pathogens. 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8923/7/fist-2015-Natanamurugaraj-Intracellular%20Biosynthesis%20Of%20Au%20And%20Ag.pdf Kuppusamy, Palaniselvam and M. M., Yusoff and Narasimha, Reddy Parine and Solachuddin J.A., Ichwan and Gaanty Pragas, Maniam and Natanamurugaraj, Govindan (2015) Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties. Journal of Environmental Sciences, 29. pp. 151-157. ISSN 1001-0742 http://dx.doi.org/10.1016/j.jes.2014.06.050 doi: 10.1016/j.jes.2014.06.050
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TD Environmental technology. Sanitary engineering
spellingShingle	TD Environmental technology. Sanitary engineering Kuppusamy, Palaniselvam M. M., Yusoff Narasimha, Reddy Parine Solachuddin J.A., Ichwan Gaanty Pragas, Maniam Natanamurugaraj, Govindan Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties
description	In this present study, we reported broccoli (Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles ( NPs) in green chemistry method . The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organisms. The physico-chemical properties of gold nanoparticles were analyzed using different analytical techniques such as a UV–Vis spectrophotometer , Field Emission Scanning Electron Micros copy, energy dispersive X-ray spectroscopy, X-ray diffraction and a Fourier Transform Infrared spectrophotometer. In addition, gold and silver NP antimicrobial efficacy was checked by disc diffusion assay. UV–Vis color intensity of the nanoparticles was shown at 540 and 450 nm for gold and silver nanoparticles respectively. Higher magnification of the Field Emission Scanning Electron Microscopy image shows the variable morphology of the gold nanoparticles such as spherical, rod and triangular shapes and silver nanop articles were seen in spherical shapes. The average spherical size of the particles was observed in 24– 38 nm for gold and 30–45 nm for silver NPs . X-ray diffraction pattern confirmed the presence of gold nanoparticles and silver nanoparticles which were crystalline in nature. Additionally , the functional metabolites were identified by the Fourier Transform Infrared spectroscopy. IR spectra revealed phenols, alcohols, aldehydes ( sugar moieties), vitamins and proteins are present in the broccoli extract which are accountable to synthesize the nanoparticles. The synthesized gold and silver NPs inhibited the growth of the tested bacterial and fungal pathogens at the concentration of 50 μg/mL respectively. Inaddition, broccoli mediated gold and silver nanoparticles have shown potent antimicrobial activity against human pathogens.
format	Article
author	Kuppusamy, Palaniselvam M. M., Yusoff Narasimha, Reddy Parine Solachuddin J.A., Ichwan Gaanty Pragas, Maniam Natanamurugaraj, Govindan
author_facet	Kuppusamy, Palaniselvam M. M., Yusoff Narasimha, Reddy Parine Solachuddin J.A., Ichwan Gaanty Pragas, Maniam Natanamurugaraj, Govindan
author_sort	Kuppusamy, Palaniselvam
title	Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties
title_short	Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties
title_full	Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties
title_fullStr	Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties
title_full_unstemmed	Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties
title_sort	intracellular biosynthesis of au and ag nanoparticles using ethanolic extract of brassica oleracea l. and studies on their physicochemical and biological properties
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/8923/ http://umpir.ump.edu.my/id/eprint/8923/ http://umpir.ump.edu.my/id/eprint/8923/ http://umpir.ump.edu.my/id/eprint/8923/7/fist-2015-Natanamurugaraj-Intracellular%20Biosynthesis%20Of%20Au%20And%20Ag.pdf
first_indexed	2023-09-18T22:06:59Z
last_indexed	2023-09-18T22:06:59Z
_version_	1777414773653110784

Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties

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