Improved Cellular Response of Chemically Crosslinked Collagen Incorporated Hydroxyethyl Cellulose/poly(vinyl) Alcohol Nanofibers Scaffold

The aim of this research is to develop biocompatible nanofibrous mats using hydroxyethyl cellulose with improved cellular adhesion profiles and stability and use these fibrous mats as potential scaffold for skin tissue engineering.Glutaraldehyde was used to treat the scaffolds water insoluble as wel...

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Main Authors:	Jahir Hussain, Fathima Shahitha, M. M., Yusoff, Farah Hanani, Zulkifli
Format:	Article
Language:	English
Published:	SAGE 2015
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/8214/ http://umpir.ump.edu.my/id/eprint/8214/ http://umpir.ump.edu.my/id/eprint/8214/ http://umpir.ump.edu.my/id/eprint/8214/1/Improved%20Cellular%20Response%20of%20Chemically%20Crosslinked%20Collagen%20Incorporated%20Hydroxyethyl%20Cellulose-Poly%28Vinyl%29%20Alcohol%20Nanofibers%20Scaffold.pdf

id	ump-8214
recordtype	eprints
spelling	ump-82142018-07-27T01:27:23Z http://umpir.ump.edu.my/id/eprint/8214/ Improved Cellular Response of Chemically Crosslinked Collagen Incorporated Hydroxyethyl Cellulose/poly(vinyl) Alcohol Nanofibers Scaffold Jahir Hussain, Fathima Shahitha M. M., Yusoff Farah Hanani, Zulkifli QD Chemistry The aim of this research is to develop biocompatible nanofibrous mats using hydroxyethyl cellulose with improved cellular adhesion profiles and stability and use these fibrous mats as potential scaffold for skin tissue engineering.Glutaraldehyde was used to treat the scaffolds water insoluble as well as improve their biostability for possible use in biomedical applications. Electrospinning of hydroxyethyl cellulose (5 wt%) with poly(vinyl alcohol) (15 wt%) incorporated with and without collagen was blended at (1:1:1) and (1:1) ratios, respectively, and was evaluated for optimal criteria as tissue engineering scaffolds. The nanofibrous mats were crosslinked and characterized by scanning electron microscope,Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetric analysis. Scanning electron microscope images showed that the mean diameters of blend nanofibers were gradually increased after chemically crosslinking with glutaraldehyde. Fourier transform infrared spectroscopy was carried out to understand chemical interactions in the presence of aldehyde groups. Thermal characterization results showed that the stability of hydroxyethyl cellulose/poly(vinyl alcohol) and hydroxyethyl cellulose/poly(vinyl alcohol)/collagen nanofibers was increased with glutaraldehyde treatment. Studies on cell–scaffolds interaction were carried out by culturing human fibroblast (hFOB) cells on the nanofibers by assessing the growth, proliferation, and morphologies of cells. The scanning electron microscope results show that better cell proliferation and attachment appeared on hydroxyethyl cellulose/poly(vinyl alcohol)/ collagen substrates after 7 days of culturing, thus, promoting the potential of electrospun scaffolds as a promising candidate for tissue engineering applications. SAGE 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8214/1/Improved%20Cellular%20Response%20of%20Chemically%20Crosslinked%20Collagen%20Incorporated%20Hydroxyethyl%20Cellulose-Poly%28Vinyl%29%20Alcohol%20Nanofibers%20Scaffold.pdf Jahir Hussain, Fathima Shahitha and M. M., Yusoff and Farah Hanani, Zulkifli (2015) Improved Cellular Response of Chemically Crosslinked Collagen Incorporated Hydroxyethyl Cellulose/poly(vinyl) Alcohol Nanofibers Scaffold. Journal of Biomaterials Applications, 29 (7). pp. 1014-1027. ISSN 0885-3282 (print); 1530-8022 (online) http://dx.doi.org/10.1177/0885328214549818 DOI: 10.1177/0885328214549818
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Jahir Hussain, Fathima Shahitha M. M., Yusoff Farah Hanani, Zulkifli Improved Cellular Response of Chemically Crosslinked Collagen Incorporated Hydroxyethyl Cellulose/poly(vinyl) Alcohol Nanofibers Scaffold
description	The aim of this research is to develop biocompatible nanofibrous mats using hydroxyethyl cellulose with improved cellular adhesion profiles and stability and use these fibrous mats as potential scaffold for skin tissue engineering.Glutaraldehyde was used to treat the scaffolds water insoluble as well as improve their biostability for possible use in biomedical applications. Electrospinning of hydroxyethyl cellulose (5 wt%) with poly(vinyl alcohol) (15 wt%) incorporated with and without collagen was blended at (1:1:1) and (1:1) ratios, respectively, and was evaluated for optimal criteria as tissue engineering scaffolds. The nanofibrous mats were crosslinked and characterized by scanning electron microscope,Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetric analysis. Scanning electron microscope images showed that the mean diameters of blend nanofibers were gradually increased after chemically crosslinking with glutaraldehyde. Fourier transform infrared spectroscopy was carried out to understand chemical interactions in the presence of aldehyde groups. Thermal characterization results showed that the stability of hydroxyethyl cellulose/poly(vinyl alcohol) and hydroxyethyl cellulose/poly(vinyl alcohol)/collagen nanofibers was increased with glutaraldehyde treatment. Studies on cell–scaffolds interaction were carried out by culturing human fibroblast (hFOB) cells on the nanofibers by assessing the growth, proliferation, and morphologies of cells. The scanning electron microscope results show that better cell proliferation and attachment appeared on hydroxyethyl cellulose/poly(vinyl alcohol)/ collagen substrates after 7 days of culturing, thus, promoting the potential of electrospun scaffolds as a promising candidate for tissue engineering applications.
format	Article
author	Jahir Hussain, Fathima Shahitha M. M., Yusoff Farah Hanani, Zulkifli
author_facet	Jahir Hussain, Fathima Shahitha M. M., Yusoff Farah Hanani, Zulkifli
author_sort	Jahir Hussain, Fathima Shahitha
title	Improved Cellular Response of Chemically Crosslinked Collagen Incorporated Hydroxyethyl Cellulose/poly(vinyl) Alcohol Nanofibers Scaffold
title_short	Improved Cellular Response of Chemically Crosslinked Collagen Incorporated Hydroxyethyl Cellulose/poly(vinyl) Alcohol Nanofibers Scaffold
title_full	Improved Cellular Response of Chemically Crosslinked Collagen Incorporated Hydroxyethyl Cellulose/poly(vinyl) Alcohol Nanofibers Scaffold
title_fullStr	Improved Cellular Response of Chemically Crosslinked Collagen Incorporated Hydroxyethyl Cellulose/poly(vinyl) Alcohol Nanofibers Scaffold
title_full_unstemmed	Improved Cellular Response of Chemically Crosslinked Collagen Incorporated Hydroxyethyl Cellulose/poly(vinyl) Alcohol Nanofibers Scaffold
title_sort	improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold
publisher	SAGE
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/8214/ http://umpir.ump.edu.my/id/eprint/8214/ http://umpir.ump.edu.my/id/eprint/8214/ http://umpir.ump.edu.my/id/eprint/8214/1/Improved%20Cellular%20Response%20of%20Chemically%20Crosslinked%20Collagen%20Incorporated%20Hydroxyethyl%20Cellulose-Poly%28Vinyl%29%20Alcohol%20Nanofibers%20Scaffold.pdf
first_indexed	2023-09-18T22:05:33Z
last_indexed	2023-09-18T22:05:33Z
_version_	1777414683736670208

Improved Cellular Response of Chemically Crosslinked Collagen Incorporated Hydroxyethyl Cellulose/poly(vinyl) Alcohol Nanofibers Scaffold

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