Optimal C:N ratio for the production of red pigments by Monascus ruber
The carbon-to-nitrogen (C:N) ratio in the biomass of microfungi tends to be quite different (e.g. 10–15) compared with the C:N ratio in the red pigments (e.g.[20) of the fungus Monascus ruber. Therefore, determining an optimal C:N ratio in the culture medium for maximizing the production of the...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Netherlands
2014
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/8089/ http://umpir.ump.edu.my/id/eprint/8089/ http://umpir.ump.edu.my/id/eprint/8089/ http://umpir.ump.edu.my/id/eprint/8089/1/Optimal%20CN%20ratio%20for%20the%20production.pdf |
| Summary: | The carbon-to-nitrogen (C:N) ratio in the biomass of microfungi tends to be quite different (e.g. 10–15)
compared with the C:N ratio in the red pigments (e.g.[20)
of the fungus Monascus ruber. Therefore, determining an
optimal C:N ratio in the culture medium for maximizing
the production of the pigments is important. A culture
medium composition is established for maximizing the
production of the red pigment by the fungus M. ruber
ICMP 15220 in submerged culture. The highest volumetric
productivity of the red pigment was 0.023 AU L-1 h-1 in
a batch culture (30 C, initial pH of 6.5) with a defined
medium of the following composition (g L-1): glucose
(10), monosodium glutamate (MSG) (10), MgSO47H2O (0.5), KH2PO4 (5), K2HPO4 (5), ZnSO47H2O (0.01),FeSO47H2O (0.01), CaCl2 (0.1), MnSO4H2O (0.03). This medium formulation had a C:N mole ratio of 9:1. Under these conditions, the specific growth rate of the fungus was 0.043 h-1 and the peak biomass concentration was 6.7 g L-1 in a 7-day culture. The biomass specific productivity of the red pigment was 1.06 AU g-1 h-1. The best nitrogen source proved to be MSG although four other inorganic nitrogen sources were evaluated. |
|---|