Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA

Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA

A label-free impedimetric DNA biosensor based on graphene nanosheets has been developed for the detection of Escherichia coli O157:H7 strain GZ-021210. Probe DNA (pDNA) of E. coli was immobilized onto graphene nanosheets by the surface functionalization of graphene with 1-pyrenebutyric acid (PyBA) f...

Full description

Bibliographic Details
Main Authors: Nurulasma, Zainudin, Mohd Hairul, Ab. Rahim, M. M., Yusoff, K. F., Chong
Format: Article
Language:English
Published: Royal Society of Chemistry 2014
Subjects:
QD Chemistry
Online Access:http://umpir.ump.edu.my/id/eprint/8061/
http://umpir.ump.edu.my/id/eprint/8061/
http://umpir.ump.edu.my/id/eprint/8061/
http://umpir.ump.edu.my/id/eprint/8061/1/Impedimetric_graphene-based_biosensor_for_the_detection_of_Escherichia_coli_DNA_%28published%29.pdf
id ump-8061
recordtype eprints
spelling ump-80612018-01-22T01:29:43Z http://umpir.ump.edu.my/id/eprint/8061/ Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA Nurulasma, Zainudin Mohd Hairul, Ab. Rahim M. M., Yusoff K. F., Chong QD Chemistry A label-free impedimetric DNA biosensor based on graphene nanosheets has been developed for the detection of Escherichia coli O157:H7 strain GZ-021210. Probe DNA (pDNA) of E. coli was immobilized onto graphene nanosheets by the surface functionalization of graphene with 1-pyrenebutyric acid (PyBA) followed by carbodiimide linkage. The hybridization of complementary DNA (cDNA) of E. coli with the immobilized pDNA increased the electron transfer resistance of the graphene nanosheets, as observed by electrochemical impedance spectroscopy (EIS). The E. coli DNA biosensor displayed a wide range of linear response (1.0 1010 M to 1.0 1014 M), low detection limit (0.7 1015 M), single-base mismatch selectivity, high robustness and good reproducibility. The current work demonstrates an important advancement in the development of a sensitive biosensor for E. coli detection. Royal Society of Chemistry 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8061/1/Impedimetric_graphene-based_biosensor_for_the_detection_of_Escherichia_coli_DNA_%28published%29.pdf Nurulasma, Zainudin and Mohd Hairul, Ab. Rahim and M. M., Yusoff and K. F., Chong (2014) Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA. Analytical Methods, 6 (19). pp. 7935-7941. ISSN 1759-9660 (print); 1759-9679 (online) http://dx.doi.org/10.1039/C4AY01836B DOI: 10.1039/C4AY01836B
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Nurulasma, Zainudin
Mohd Hairul, Ab. Rahim
M. M., Yusoff
K. F., Chong
Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA
description A label-free impedimetric DNA biosensor based on graphene nanosheets has been developed for the detection of Escherichia coli O157:H7 strain GZ-021210. Probe DNA (pDNA) of E. coli was immobilized onto graphene nanosheets by the surface functionalization of graphene with 1-pyrenebutyric acid (PyBA) followed by carbodiimide linkage. The hybridization of complementary DNA (cDNA) of E. coli with the immobilized pDNA increased the electron transfer resistance of the graphene nanosheets, as observed by electrochemical impedance spectroscopy (EIS). The E. coli DNA biosensor displayed a wide range of linear response (1.0 1010 M to 1.0 1014 M), low detection limit (0.7 1015 M), single-base mismatch selectivity, high robustness and good reproducibility. The current work demonstrates an important advancement in the development of a sensitive biosensor for E. coli detection.
format Article
author Nurulasma, Zainudin
Mohd Hairul, Ab. Rahim
M. M., Yusoff
K. F., Chong
author_facet Nurulasma, Zainudin
Mohd Hairul, Ab. Rahim
M. M., Yusoff
K. F., Chong
author_sort Nurulasma, Zainudin
title Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA
title_short Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA
title_full Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA
title_fullStr Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA
title_full_unstemmed Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA
title_sort impedimetric graphene-based biosensor for the detection of escherichia coli dna
publisher Royal Society of Chemistry
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/8061/
http://umpir.ump.edu.my/id/eprint/8061/
http://umpir.ump.edu.my/id/eprint/8061/
http://umpir.ump.edu.my/id/eprint/8061/1/Impedimetric_graphene-based_biosensor_for_the_detection_of_Escherichia_coli_DNA_%28published%29.pdf
first_indexed 2023-09-18T22:05:20Z
last_indexed 2023-09-18T22:05:20Z
_version_ 1777414670483718144

Similar Items