Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source

Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source

Poly(3-hydroxybutyrate) (PHB) is a biodegradable polymer that can be synthesized through bacterial fermentation. In this study, Cupriavidus necator H16 is used to synthesize PHB by using Jatropha oil as its sole carbon source. Different variables mainly jatropha oil and urea concentrations, and agit...

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Main Authors: Abeed Fatima, Mohidin Batcha, D. M. R., Prasad, Khan, Maksudur R., Hamidah, Abdullah
Format: Article
Language:English
English
Published: Springer Berlin Heidelberg 2014
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/8060/
http://umpir.ump.edu.my/id/eprint/8060/
http://umpir.ump.edu.my/id/eprint/8060/
http://umpir.ump.edu.my/id/eprint/8060/1/Biosynthesis_of_poly_%283-hydroxybutyrate%29%28PHB%29_by_Cupriavidus_necator_H16_from_jatropha_oil_as_carbon_source.pdf
http://umpir.ump.edu.my/id/eprint/8060/4/Biosynthesis%20of%20Poly%283-Hydroxybutyrate%29%20%28Phb%29%20by%20Cupriavidus%20Necator%20H16%20From%20Jatropha%20Oil%20As%20Carbon%20Source.pdf
id ump-8060
recordtype eprints
spelling ump-80602017-12-15T08:06:34Z http://umpir.ump.edu.my/id/eprint/8060/ Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source Abeed Fatima, Mohidin Batcha D. M. R., Prasad Khan, Maksudur R. Hamidah, Abdullah TP Chemical technology Poly(3-hydroxybutyrate) (PHB) is a biodegradable polymer that can be synthesized through bacterial fermentation. In this study, Cupriavidus necator H16 is used to synthesize PHB by using Jatropha oil as its sole carbon source. Different variables mainly jatropha oil and urea concentrations, and agitation rate were investigated to determine the optimum condition for microbial fermentation in batch culture. Based on the results, the highest cell dry weight and PHB concentrations of 20.1 and 15.5 g/L, respectively, were obtained when 20 g/L of jatropha oil was used. Ethanol was used as external stress factor and the addition of 1.5 % ethanol at 38 h had a positive effect with a high PHB yield of 0.987 g PHB/g jatropha oil. The kinetic studies for cell growth rate and PHB production were conducted and the data were fitted with Logistic and Leudeking–Piret models. The rate constants were evaluated and the theoretical values were in accordance with the experimental data obtained. Springer Berlin Heidelberg 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8060/1/Biosynthesis_of_poly_%283-hydroxybutyrate%29%28PHB%29_by_Cupriavidus_necator_H16_from_jatropha_oil_as_carbon_source.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/8060/4/Biosynthesis%20of%20Poly%283-Hydroxybutyrate%29%20%28Phb%29%20by%20Cupriavidus%20Necator%20H16%20From%20Jatropha%20Oil%20As%20Carbon%20Source.pdf Abeed Fatima, Mohidin Batcha and D. M. R., Prasad and Khan, Maksudur R. and Hamidah, Abdullah (2014) Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source. Bioprocess and Biosystems Engineering, 37 (5). pp. 943-951. ISSN 1615-7591 (print); 1615-7605 (online) http://dx.doi.org/10.1007/s00449-013-1066-4 DOI: 10.1007/s00449-013-1066-4
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Abeed Fatima, Mohidin Batcha
D. M. R., Prasad
Khan, Maksudur R.
Hamidah, Abdullah
Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source
description Poly(3-hydroxybutyrate) (PHB) is a biodegradable polymer that can be synthesized through bacterial fermentation. In this study, Cupriavidus necator H16 is used to synthesize PHB by using Jatropha oil as its sole carbon source. Different variables mainly jatropha oil and urea concentrations, and agitation rate were investigated to determine the optimum condition for microbial fermentation in batch culture. Based on the results, the highest cell dry weight and PHB concentrations of 20.1 and 15.5 g/L, respectively, were obtained when 20 g/L of jatropha oil was used. Ethanol was used as external stress factor and the addition of 1.5 % ethanol at 38 h had a positive effect with a high PHB yield of 0.987 g PHB/g jatropha oil. The kinetic studies for cell growth rate and PHB production were conducted and the data were fitted with Logistic and Leudeking–Piret models. The rate constants were evaluated and the theoretical values were in accordance with the experimental data obtained.
format Article
author Abeed Fatima, Mohidin Batcha
D. M. R., Prasad
Khan, Maksudur R.
Hamidah, Abdullah
author_facet Abeed Fatima, Mohidin Batcha
D. M. R., Prasad
Khan, Maksudur R.
Hamidah, Abdullah
author_sort Abeed Fatima, Mohidin Batcha
title Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source
title_short Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source
title_full Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source
title_fullStr Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source
title_full_unstemmed Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source
title_sort biosynthesis of poly (3-hydroxybutyrate)(phb) by cupriavidus necator h16 from jatropha oil as carbon source
publisher Springer Berlin Heidelberg
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/8060/
http://umpir.ump.edu.my/id/eprint/8060/
http://umpir.ump.edu.my/id/eprint/8060/
http://umpir.ump.edu.my/id/eprint/8060/1/Biosynthesis_of_poly_%283-hydroxybutyrate%29%28PHB%29_by_Cupriavidus_necator_H16_from_jatropha_oil_as_carbon_source.pdf
http://umpir.ump.edu.my/id/eprint/8060/4/Biosynthesis%20of%20Poly%283-Hydroxybutyrate%29%20%28Phb%29%20by%20Cupriavidus%20Necator%20H16%20From%20Jatropha%20Oil%20As%20Carbon%20Source.pdf
first_indexed 2023-09-18T22:05:20Z
last_indexed 2023-09-18T22:05:20Z
_version_ 1777414670327480320

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