Production Of Xylose Reductase From Adapted Candida Tropicalis Grown In Sawdust Hydrolysate

Xylose, recovered from Meranti wood sawdust (MWS), can be used as a promising and alternative carbon source for yeast growth as well as for the production of xylose reductase (XR). This enzyme has potential applications in the bioproduction of various high value products, especially xylitol. The a...

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Main Authors:	M. Rafiqul, Islam, Mimi Sakinah, A. M.
Format:	Article
Language:	English
Published:	Elsevier Ltd. 2014
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/7995/ http://umpir.ump.edu.my/id/eprint/7995/ http://umpir.ump.edu.my/id/eprint/7995/ http://umpir.ump.edu.my/id/eprint/7995/1/fkksa-2014-mimi-Production%20of%20xylose.pdf

id	ump-7995
recordtype	eprints
spelling	ump-79952018-11-29T07:26:59Z http://umpir.ump.edu.my/id/eprint/7995/ Production Of Xylose Reductase From Adapted Candida Tropicalis Grown In Sawdust Hydrolysate M. Rafiqul, Islam Mimi Sakinah, A. M. TP Chemical technology Xylose, recovered from Meranti wood sawdust (MWS), can be used as a promising and alternative carbon source for yeast growth as well as for the production of xylose reductase (XR). This enzyme has potential applications in the bioproduction of various high value products, especially xylitol. The aim of this study was to isolate XR from adapted yeast Candida tropicalis and to characterize it. The XR enzyme was prepared from C. tropicalis strain, grown in MWS hydrolysate-based medium, by ultrasonic homogenization. The isolated XR was characterized based on enzyme activity, stability, and kinetic constants. The activity of NADPH-dependent crude XR measured was 11.16 U/mL. It was stable at pH 5.0–7.0 and temperature of 25–40 1C for 24 h, and retained above 95% of its original activity after 4 months of storage at –80 1C. The apparent Km values of XR for xylose and NADPH were 81.78 mM and 7.29 μM while the Vmax for xylose and NADPH were 178.57 and 12.5 μM/min, respectively. The low Km,app and high Vmax,app values of XR for xylose as a substrate indicates a strong binding affinity for xylose and good productivity of the reaction. Elsevier Ltd. 2014-06-02 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7995/1/fkksa-2014-mimi-Production%20of%20xylose.pdf M. Rafiqul, Islam and Mimi Sakinah, A. M. (2014) Production Of Xylose Reductase From Adapted Candida Tropicalis Grown In Sawdust Hydrolysate. Biocatalysis and Agricultural Biotechnology, 3. pp. 227-235. ISSN 1878-8181 http://dx.doi.org/10.1016/j.bcab.2014.05.003 DOI: 10.1016/j.bcab.2014.05.003
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology M. Rafiqul, Islam Mimi Sakinah, A. M. Production Of Xylose Reductase From Adapted Candida Tropicalis Grown In Sawdust Hydrolysate
description	Xylose, recovered from Meranti wood sawdust (MWS), can be used as a promising and alternative carbon source for yeast growth as well as for the production of xylose reductase (XR). This enzyme has potential applications in the bioproduction of various high value products, especially xylitol. The aim of this study was to isolate XR from adapted yeast Candida tropicalis and to characterize it. The XR enzyme was prepared from C. tropicalis strain, grown in MWS hydrolysate-based medium, by ultrasonic homogenization. The isolated XR was characterized based on enzyme activity, stability, and kinetic constants. The activity of NADPH-dependent crude XR measured was 11.16 U/mL. It was stable at pH 5.0–7.0 and temperature of 25–40 1C for 24 h, and retained above 95% of its original activity after 4 months of storage at –80 1C. The apparent Km values of XR for xylose and NADPH were 81.78 mM and 7.29 μM while the Vmax for xylose and NADPH were 178.57 and 12.5 μM/min, respectively. The low Km,app and high Vmax,app values of XR for xylose as a substrate indicates a strong binding affinity for xylose and good productivity of the reaction.
format	Article
author	M. Rafiqul, Islam Mimi Sakinah, A. M.
author_facet	M. Rafiqul, Islam Mimi Sakinah, A. M.
author_sort	M. Rafiqul, Islam
title	Production Of Xylose Reductase From Adapted Candida Tropicalis Grown In Sawdust Hydrolysate
title_short	Production Of Xylose Reductase From Adapted Candida Tropicalis Grown In Sawdust Hydrolysate
title_full	Production Of Xylose Reductase From Adapted Candida Tropicalis Grown In Sawdust Hydrolysate
title_fullStr	Production Of Xylose Reductase From Adapted Candida Tropicalis Grown In Sawdust Hydrolysate
title_full_unstemmed	Production Of Xylose Reductase From Adapted Candida Tropicalis Grown In Sawdust Hydrolysate
title_sort	production of xylose reductase from adapted candida tropicalis grown in sawdust hydrolysate
publisher	Elsevier Ltd.
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/7995/ http://umpir.ump.edu.my/id/eprint/7995/ http://umpir.ump.edu.my/id/eprint/7995/ http://umpir.ump.edu.my/id/eprint/7995/1/fkksa-2014-mimi-Production%20of%20xylose.pdf
first_indexed	2023-09-18T22:05:13Z
last_indexed	2023-09-18T22:05:13Z
_version_	1777414663021002752

Production Of Xylose Reductase From Adapted Candida Tropicalis Grown In Sawdust Hydrolysate

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