Inhibition By Toxic Compounds In The Hemicellulosic Hydrolysates On The Activity Of Xylose Reductase From Candida Tropicalis
Xylose reductase (XR) is an oxidoreductase having potential applications in the production of various specialty products, mainly xylitol. It is important to screen for compounds that can decrease XR activity and consequently can decrease xylitol production. We have identified the byproducts in...
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Springer Netherlands
2014
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ump-79932018-11-29T07:26:06Z http://umpir.ump.edu.my/id/eprint/7993/ Inhibition By Toxic Compounds In The Hemicellulosic Hydrolysates On The Activity Of Xylose Reductase From Candida Tropicalis M. Rafiqul, Islam Mimi Sakinah, A. M. Zularisam, A. W. TP Chemical technology Xylose reductase (XR) is an oxidoreductase having potential applications in the production of various specialty products, mainly xylitol. It is important to screen for compounds that can decrease XR activity and consequently can decrease xylitol production. We have identified the byproducts in the hemicellulosic hydrolysate that inhibit XR from Candida tropicalis and measured their effects. XR inhibitory activities of byproducts, glucose, acetic acid, arabinose, lignin-degradation products (LDPs), furfural and hydroxymethylfurfural (HMF), were evaluated by measuring the MIC and IC50 values. XR activity was 11.2 U/ml. Acetic acid, LDPs, furfural and HMF significantly inhibited XR with IC50 values of 11, 6.4, 2.3 and 0.4 g/l, respectively. This is the first report on the inhibitory activities of several byproducts for XR. Springer Netherlands 2014-09-12 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7993/1/Rafiq-2014b_%28BILE%29_%281%29.pdf M. Rafiqul, Islam and Mimi Sakinah, A. M. and Zularisam, A. W. (2014) Inhibition By Toxic Compounds In The Hemicellulosic Hydrolysates On The Activity Of Xylose Reductase From Candida Tropicalis. Biotechnology Letters, 37 (1). pp. 191-196. ISSN 1573-6776 http://dx.doi.org/10.1007/s10529-014-1672-5 DOI: 10.1007/s10529-014-1672-5 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Malaysia Pahang |
| building |
UMP Institutional Repository |
| collection |
Online Access |
| language |
English |
| topic |
TP Chemical technology |
| spellingShingle |
TP Chemical technology M. Rafiqul, Islam Mimi Sakinah, A. M. Zularisam, A. W. Inhibition By Toxic Compounds In The Hemicellulosic Hydrolysates On The Activity Of Xylose Reductase From Candida Tropicalis |
| description |
Xylose reductase (XR) is an oxidoreductase
having potential applications in the production of
various specialty products, mainly xylitol. It is
important to screen for compounds that can decrease
XR activity and consequently can decrease xylitol
production. We have identified the byproducts in the
hemicellulosic hydrolysate that inhibit XR from
Candida tropicalis and measured their effects. XR
inhibitory activities of byproducts, glucose, acetic
acid, arabinose, lignin-degradation products (LDPs),
furfural and hydroxymethylfurfural (HMF), were
evaluated by measuring the MIC and IC50 values.
XR activity was 11.2 U/ml. Acetic acid, LDPs,
furfural and HMF significantly inhibited XR with
IC50 values of 11, 6.4, 2.3 and 0.4 g/l, respectively.
This is the first report on the inhibitory activities of
several byproducts for XR. |
| format |
Article |
| author |
M. Rafiqul, Islam Mimi Sakinah, A. M. Zularisam, A. W. |
| author_facet |
M. Rafiqul, Islam Mimi Sakinah, A. M. Zularisam, A. W. |
| author_sort |
M. Rafiqul, Islam |
| title |
Inhibition By Toxic Compounds In The Hemicellulosic Hydrolysates On The Activity Of Xylose Reductase From Candida Tropicalis |
| title_short |
Inhibition By Toxic Compounds In The Hemicellulosic Hydrolysates On The Activity Of Xylose Reductase From Candida Tropicalis |
| title_full |
Inhibition By Toxic Compounds In The Hemicellulosic Hydrolysates On The Activity Of Xylose Reductase From Candida Tropicalis |
| title_fullStr |
Inhibition By Toxic Compounds In The Hemicellulosic Hydrolysates On The Activity Of Xylose Reductase From Candida Tropicalis |
| title_full_unstemmed |
Inhibition By Toxic Compounds In The Hemicellulosic Hydrolysates On The Activity Of Xylose Reductase From Candida Tropicalis |
| title_sort |
inhibition by toxic compounds in the hemicellulosic hydrolysates on the activity of xylose reductase from candida tropicalis |
| publisher |
Springer Netherlands |
| publishDate |
2014 |
| url |
http://umpir.ump.edu.my/id/eprint/7993/ http://umpir.ump.edu.my/id/eprint/7993/ http://umpir.ump.edu.my/id/eprint/7993/ http://umpir.ump.edu.my/id/eprint/7993/1/Rafiq-2014b_%28BILE%29_%281%29.pdf |
| first_indexed |
2023-09-18T22:05:13Z |
| last_indexed |
2023-09-18T22:05:13Z |
| _version_ |
1777414662725304320 |