Scale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L

Scale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L

In this research is about the fermentation process for PHB production by Cupriavidus necator using glucose as a substrate. The different parameters of glucose concentration and agitation speed were studied. There are two stages in this research. For the first stage, the scale-up process was done fro...

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Main Author: Mohd Hishafi, Husin
Format: Undergraduates Project Papers
Language:English
Published: 2009
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/781/
http://umpir.ump.edu.my/id/eprint/781/
http://umpir.ump.edu.my/id/eprint/781/1/cd4013.pdf
id ump-781
recordtype eprints
spelling ump-7812015-03-03T07:46:51Z http://umpir.ump.edu.my/id/eprint/781/ Scale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L Mohd Hishafi, Husin TP Chemical technology In this research is about the fermentation process for PHB production by Cupriavidus necator using glucose as a substrate. The different parameters of glucose concentration and agitation speed were studied. There are two stages in this research. For the first stage, the scale-up process was done from shake flask to 10L fermenter based on constant volumetric mass transfer coefficient KLa. The shake flask agitation speed of 200 rpm and temperature 30°C were used as the basis of scale up. The result gives the PHB yield for shake flask and 10L as 60.12mg/L and 60.19 mg/L respectively. From these results, it can be concluded that the first stage had achieved its objective. For the second stage, optimization of variables was done by using glucose concentration; 10g/L and 30g/L and agitation speed; 200 rpm and 260 rpm as the manipulated variables. It was observed that the PHB yield is affected by initial glucose concentration of glucose used and also by the agitation speed. 2009-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/781/1/cd4013.pdf Mohd Hishafi, Husin (2009) Scale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:45142&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mohd Hishafi, Husin
Scale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L
description In this research is about the fermentation process for PHB production by Cupriavidus necator using glucose as a substrate. The different parameters of glucose concentration and agitation speed were studied. There are two stages in this research. For the first stage, the scale-up process was done from shake flask to 10L fermenter based on constant volumetric mass transfer coefficient KLa. The shake flask agitation speed of 200 rpm and temperature 30°C were used as the basis of scale up. The result gives the PHB yield for shake flask and 10L as 60.12mg/L and 60.19 mg/L respectively. From these results, it can be concluded that the first stage had achieved its objective. For the second stage, optimization of variables was done by using glucose concentration; 10g/L and 30g/L and agitation speed; 200 rpm and 260 rpm as the manipulated variables. It was observed that the PHB yield is affected by initial glucose concentration of glucose used and also by the agitation speed.
format Undergraduates Project Papers
author Mohd Hishafi, Husin
author_facet Mohd Hishafi, Husin
author_sort Mohd Hishafi, Husin
title Scale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L
title_short Scale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L
title_full Scale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L
title_fullStr Scale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L
title_full_unstemmed Scale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L
title_sort scale up of biopolymer (phb) fermentation from shake flask to 10l stirred tank fermenter and optimization of levels of variables at 10l
publishDate 2009
url http://umpir.ump.edu.my/id/eprint/781/
http://umpir.ump.edu.my/id/eprint/781/
http://umpir.ump.edu.my/id/eprint/781/1/cd4013.pdf
first_indexed 2023-09-18T21:53:20Z
last_indexed 2023-09-18T21:53:20Z
_version_ 1777413914707886080

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