Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology

Xylanase has been used widely in industries today due to its ability in catalyzing various types of biochemical reactions. However, production cost of xylanase enzyme is very expensive. A research to reduce the cost by optimization of temperature and agitation rate on expression of recombinant xylan...

Full description

Bibliographic Details
Main Author:	Nur Azhar, Saari
Format:	Undergraduates Project Papers
Language:	English
Published:	2009
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/780/ http://umpir.ump.edu.my/id/eprint/780/ http://umpir.ump.edu.my/id/eprint/780/1/cd4039.pdf

id	ump-780
recordtype	eprints
spelling	ump-7802015-03-03T07:46:51Z http://umpir.ump.edu.my/id/eprint/780/ Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology Nur Azhar, Saari TP Chemical technology Xylanase has been used widely in industries today due to its ability in catalyzing various types of biochemical reactions. However, production cost of xylanase enzyme is very expensive. A research to reduce the cost by optimization of temperature and agitation rate on expression of recombinant xylanase in K. lactis using Response Surface Methodology (RSM) was successfully done. At the early stage of experiment, one factor at a time was employed to screen the best range for temperature and agitation rate. All the parameter ranges obtained were used in Response Surface Methodology (RSM). Response Surface Methodology in Design Expert version 7.1.6 software was used with Central Composite Design (CCD) mode. Thirteen sets of experiments with different parameter values were suggested by the software. The predicted optimum values for temperature, agitation rate and xylanase activities were 34.68oC, 253 rpm and 9.198 U/ml respectively. One set of experiment was run using the optimized parameter and as a result, 9.221 U/ml xylanase activity was recorded. Before optimization, xylanase activity was only 5.221 U/ml and the activity was increased by 43.75% or 1.78-fold after optimization. The optimization also reduces the energy consumption as the temperature was reduced from 38oC to 34.6oC and agitation rate reduced from 280 to 253 rpm. As conclusion, this research is successful to increase recombinant xylanase production, reduce the energy consumption and also able to reduce the production cost.-Author- 2009-05 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/780/1/cd4039.pdf Nur Azhar, Saari (2009) Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:45194&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nur Azhar, Saari Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology
description	Xylanase has been used widely in industries today due to its ability in catalyzing various types of biochemical reactions. However, production cost of xylanase enzyme is very expensive. A research to reduce the cost by optimization of temperature and agitation rate on expression of recombinant xylanase in K. lactis using Response Surface Methodology (RSM) was successfully done. At the early stage of experiment, one factor at a time was employed to screen the best range for temperature and agitation rate. All the parameter ranges obtained were used in Response Surface Methodology (RSM). Response Surface Methodology in Design Expert version 7.1.6 software was used with Central Composite Design (CCD) mode. Thirteen sets of experiments with different parameter values were suggested by the software. The predicted optimum values for temperature, agitation rate and xylanase activities were 34.68oC, 253 rpm and 9.198 U/ml respectively. One set of experiment was run using the optimized parameter and as a result, 9.221 U/ml xylanase activity was recorded. Before optimization, xylanase activity was only 5.221 U/ml and the activity was increased by 43.75% or 1.78-fold after optimization. The optimization also reduces the energy consumption as the temperature was reduced from 38oC to 34.6oC and agitation rate reduced from 280 to 253 rpm. As conclusion, this research is successful to increase recombinant xylanase production, reduce the energy consumption and also able to reduce the production cost.-Author-
format	Undergraduates Project Papers
author	Nur Azhar, Saari
author_facet	Nur Azhar, Saari
author_sort	Nur Azhar, Saari
title	Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology
title_short	Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology
title_full	Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology
title_fullStr	Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology
title_full_unstemmed	Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology
title_sort	optimization of temperature and agitation rate on expression of recombinant xylanase in kluyveromyces lactics using response surfacemethodology
publishDate	2009
url	http://umpir.ump.edu.my/id/eprint/780/ http://umpir.ump.edu.my/id/eprint/780/ http://umpir.ump.edu.my/id/eprint/780/1/cd4039.pdf
first_indexed	2023-09-18T21:53:19Z
last_indexed	2023-09-18T21:53:19Z
_version_	1777413914558988288

Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology

Similar Items