Determination of stevioside and rebaudioside a in Stevia rebaudiana leaves via preparative HPLC

Stevia rebaudiana leaves, also known as sweet leaf. It has been used as sweetener for centuries in South America. Stevia rebaudiana leaves contain noncaloric sweeteners (steviol glycosides) whose consumption could exert beneficial effects on human health. Steviol glycosides are considered safe. The...

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Main Author:	Aliaa Diyana, Abdul Hisham
Format:	Undergraduates Project Papers
Language:	English
Published:	2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/7782/ http://umpir.ump.edu.my/id/eprint/7782/ http://umpir.ump.edu.my/id/eprint/7782/1/ALIAA_DIYANA_BINTI_ABDUL_HISHAM.PDF

id	ump-7782
recordtype	eprints
spelling	ump-77822016-04-20T02:55:31Z http://umpir.ump.edu.my/id/eprint/7782/ Determination of stevioside and rebaudioside a in Stevia rebaudiana leaves via preparative HPLC Aliaa Diyana, Abdul Hisham TP Chemical technology Stevia rebaudiana leaves, also known as sweet leaf. It has been used as sweetener for centuries in South America. Stevia rebaudiana leaves contain noncaloric sweeteners (steviol glycosides) whose consumption could exert beneficial effects on human health. Steviol glycosides are considered safe. The aim of present study was to determine qualitative of stevioside and rebaudioside A which compounds of steviol glycosides consist in Stevia leaves by soxhiet extraction method using Waters preparative HPLC autopurification system. Samples were sequentially extracted by soxhiet extraction method using methanol solvent and the analytes separated by Waters XBridge C 18 column (150 mm x 4.6 mm I.D., 5tm). This HPLC analytical column, performed with a mobile phase in isocratic mode elution consisting of acetonitrile/water (80:20 v/v). Stevioside were the most abundant steviol glycosides found followed rebaudioside A in samples of Stevia (n = 5) from Malaysia. This proposed Waters preparative HPLC autopurification system can be applied for further analysis in this study for the routine quality control of Stevia leaves and their commercial preparations. 2012 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7782/1/ALIAA_DIYANA_BINTI_ABDUL_HISHAM.PDF Aliaa Diyana, Abdul Hisham (2012) Determination of stevioside and rebaudioside a in Stevia rebaudiana leaves via preparative HPLC. Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:76201&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Aliaa Diyana, Abdul Hisham Determination of stevioside and rebaudioside a in Stevia rebaudiana leaves via preparative HPLC
description	Stevia rebaudiana leaves, also known as sweet leaf. It has been used as sweetener for centuries in South America. Stevia rebaudiana leaves contain noncaloric sweeteners (steviol glycosides) whose consumption could exert beneficial effects on human health. Steviol glycosides are considered safe. The aim of present study was to determine qualitative of stevioside and rebaudioside A which compounds of steviol glycosides consist in Stevia leaves by soxhiet extraction method using Waters preparative HPLC autopurification system. Samples were sequentially extracted by soxhiet extraction method using methanol solvent and the analytes separated by Waters XBridge C 18 column (150 mm x 4.6 mm I.D., 5tm). This HPLC analytical column, performed with a mobile phase in isocratic mode elution consisting of acetonitrile/water (80:20 v/v). Stevioside were the most abundant steviol glycosides found followed rebaudioside A in samples of Stevia (n = 5) from Malaysia. This proposed Waters preparative HPLC autopurification system can be applied for further analysis in this study for the routine quality control of Stevia leaves and their commercial preparations.
format	Undergraduates Project Papers
author	Aliaa Diyana, Abdul Hisham
author_facet	Aliaa Diyana, Abdul Hisham
author_sort	Aliaa Diyana, Abdul Hisham
title	Determination of stevioside and rebaudioside a in Stevia rebaudiana leaves via preparative HPLC
title_short	Determination of stevioside and rebaudioside a in Stevia rebaudiana leaves via preparative HPLC
title_full	Determination of stevioside and rebaudioside a in Stevia rebaudiana leaves via preparative HPLC
title_fullStr	Determination of stevioside and rebaudioside a in Stevia rebaudiana leaves via preparative HPLC
title_full_unstemmed	Determination of stevioside and rebaudioside a in Stevia rebaudiana leaves via preparative HPLC
title_sort	determination of stevioside and rebaudioside a in stevia rebaudiana leaves via preparative hplc
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/7782/ http://umpir.ump.edu.my/id/eprint/7782/ http://umpir.ump.edu.my/id/eprint/7782/1/ALIAA_DIYANA_BINTI_ABDUL_HISHAM.PDF
first_indexed	2023-09-18T22:04:46Z
last_indexed	2023-09-18T22:04:46Z
_version_	1777414634040459264

Determination of stevioside and rebaudioside a in Stevia rebaudiana leaves via preparative HPLC

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