Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)

Xylose is found in the embryos of most edible plants. Xylan, the hemicellulose used as raw material in xylose production. Xylanase, the enzyme that is used to hydrolyzed the structure of xylan to produce xylose. This study aims to improve the production of xylose from Palm Oil Empty Fruit Bunch (POE...

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Main Author:	Asyuaraa, Sukmarsari
Format:	Undergraduates Project Papers
Language:	English
Published:	2009
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/775/ http://umpir.ump.edu.my/id/eprint/775/1/cd4016.pdf

id	ump-775
recordtype	eprints
spelling	ump-7752015-03-03T07:46:49Z http://umpir.ump.edu.my/id/eprint/775/ Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM) Asyuaraa, Sukmarsari TP Chemical technology Xylose is found in the embryos of most edible plants. Xylan, the hemicellulose used as raw material in xylose production. Xylanase, the enzyme that is used to hydrolyzed the structure of xylan to produce xylose. This study aims to improve the production of xylose from Palm Oil Empty Fruit Bunch (POEFB) using enzymatic hydrolysis process. The effect of cultural conditions such as enzyme concentration and incubation time are used to study the production of xylose. Three different xylanases enzyme are used to produce xylose namely Multifect Xylanase, Multifect CX 12L and Optimase CX 72L. Multifect CX 12L was the best enzyme that yielded the most xylose.The optimization of the production of xylose is carried out by using response surface methodology (RSM) based on the central composite design (CCD). The optimal set cultural conditions for enzyme concentration are 50 U/g and 35.5 hours of incubation. Xylose production of 23.15 mg/ml was achieved by using these optimal conditions. 2009-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/775/1/cd4016.pdf Asyuaraa, Sukmarsari (2009) Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM). Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Asyuaraa, Sukmarsari Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
description	Xylose is found in the embryos of most edible plants. Xylan, the hemicellulose used as raw material in xylose production. Xylanase, the enzyme that is used to hydrolyzed the structure of xylan to produce xylose. This study aims to improve the production of xylose from Palm Oil Empty Fruit Bunch (POEFB) using enzymatic hydrolysis process. The effect of cultural conditions such as enzyme concentration and incubation time are used to study the production of xylose. Three different xylanases enzyme are used to produce xylose namely Multifect Xylanase, Multifect CX 12L and Optimase CX 72L. Multifect CX 12L was the best enzyme that yielded the most xylose.The optimization of the production of xylose is carried out by using response surface methodology (RSM) based on the central composite design (CCD). The optimal set cultural conditions for enzyme concentration are 50 U/g and 35.5 hours of incubation. Xylose production of 23.15 mg/ml was achieved by using these optimal conditions.
format	Undergraduates Project Papers
author	Asyuaraa, Sukmarsari
author_facet	Asyuaraa, Sukmarsari
author_sort	Asyuaraa, Sukmarsari
title	Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title_short	Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title_full	Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title_fullStr	Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title_full_unstemmed	Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title_sort	effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (rsm)
publishDate	2009
url	http://umpir.ump.edu.my/id/eprint/775/ http://umpir.ump.edu.my/id/eprint/775/1/cd4016.pdf
first_indexed	2023-09-18T21:53:19Z
last_indexed	2023-09-18T21:53:19Z
_version_	1777413913817645056

Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)

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