Study on the effect of ultrasound on cellulose hydrolysis by cellulase

Cellulose is a natural polymer that has a potential of utilization of cellulosic biomass as a renewable resource for reducing emissions of carbon dioxide and to be used as future fuis :such as ethanol and other chemical products. The study on the effect of ultrasound on cellulose hydrolysis by cellu...

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Main Author:	Siti Hajar , Zerry @ Azhari
Format:	Undergraduates Project Papers
Language:	English
Published:	2013
Subjects:	QP Physiology
Online Access:	http://umpir.ump.edu.my/id/eprint/7571/ http://umpir.ump.edu.my/id/eprint/7571/ http://umpir.ump.edu.my/id/eprint/7571/1/SITI_HAJAR_BINTI_ZERRY_%40_AZHARI.PDF

id	ump-7571
recordtype	eprints
spelling	ump-75712015-03-03T09:36:50Z http://umpir.ump.edu.my/id/eprint/7571/ Study on the effect of ultrasound on cellulose hydrolysis by cellulase Siti Hajar , Zerry @ Azhari QP Physiology Cellulose is a natural polymer that has a potential of utilization of cellulosic biomass as a renewable resource for reducing emissions of carbon dioxide and to be used as future fuis :such as ethanol and other chemical products. The study on the effect of ultrasound on cellulose hydrolysis by cellulase was to be conducted. The purpose of the study is to determine the optimum condition of sonication regimen in enzymatic hydrolysis of cellulose, determine the best parameters of sonication powers and duty cycle for enzymatic hydrolysis using Michaelis-Menten kinetics and study the effect of substrate particle size (sigmacell cellulose) on the rate of reaction (solid liquid mass transfer effect). The method of this research include the preparation of substrate by dissolving the powder in 500m1 of 0.05 M acetate buffer, pH 4.8, hydrolysis of cellulose in a 2 L stirred beaker, sonication amplitude for ultrasound-assisted hydrolysis, testing the cellulase stability and activity, and also Dinitrosalicylic Acid (DNS) method for analysis. From the experiment, it can be conclude that both hydrolysis of soluble and insoluble cellulose followed Michealis Menten kinetics model. Besides that, it is proved that sonication always enhances rate of product formation regardless of substrate particle size. In contrast, an increasing particle size reduced the rate of hydrolysis regardless of implied sonication. 2013-02 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7571/1/SITI_HAJAR_BINTI_ZERRY_%40_AZHARI.PDF Siti Hajar , Zerry @ Azhari (2013) Study on the effect of ultrasound on cellulose hydrolysis by cellulase. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:75423&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QP Physiology
spellingShingle	QP Physiology Siti Hajar , Zerry @ Azhari Study on the effect of ultrasound on cellulose hydrolysis by cellulase
description	Cellulose is a natural polymer that has a potential of utilization of cellulosic biomass as a renewable resource for reducing emissions of carbon dioxide and to be used as future fuis :such as ethanol and other chemical products. The study on the effect of ultrasound on cellulose hydrolysis by cellulase was to be conducted. The purpose of the study is to determine the optimum condition of sonication regimen in enzymatic hydrolysis of cellulose, determine the best parameters of sonication powers and duty cycle for enzymatic hydrolysis using Michaelis-Menten kinetics and study the effect of substrate particle size (sigmacell cellulose) on the rate of reaction (solid liquid mass transfer effect). The method of this research include the preparation of substrate by dissolving the powder in 500m1 of 0.05 M acetate buffer, pH 4.8, hydrolysis of cellulose in a 2 L stirred beaker, sonication amplitude for ultrasound-assisted hydrolysis, testing the cellulase stability and activity, and also Dinitrosalicylic Acid (DNS) method for analysis. From the experiment, it can be conclude that both hydrolysis of soluble and insoluble cellulose followed Michealis Menten kinetics model. Besides that, it is proved that sonication always enhances rate of product formation regardless of substrate particle size. In contrast, an increasing particle size reduced the rate of hydrolysis regardless of implied sonication.
format	Undergraduates Project Papers
author	Siti Hajar , Zerry @ Azhari
author_facet	Siti Hajar , Zerry @ Azhari
author_sort	Siti Hajar , Zerry @ Azhari
title	Study on the effect of ultrasound on cellulose hydrolysis by cellulase
title_short	Study on the effect of ultrasound on cellulose hydrolysis by cellulase
title_full	Study on the effect of ultrasound on cellulose hydrolysis by cellulase
title_fullStr	Study on the effect of ultrasound on cellulose hydrolysis by cellulase
title_full_unstemmed	Study on the effect of ultrasound on cellulose hydrolysis by cellulase
title_sort	study on the effect of ultrasound on cellulose hydrolysis by cellulase
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/7571/ http://umpir.ump.edu.my/id/eprint/7571/ http://umpir.ump.edu.my/id/eprint/7571/1/SITI_HAJAR_BINTI_ZERRY_%40_AZHARI.PDF
first_indexed	2023-09-18T22:04:18Z
last_indexed	2023-09-18T22:04:18Z
_version_	1777414604859637760

Study on the effect of ultrasound on cellulose hydrolysis by cellulase

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