Immobilization Strategy of Recombinant Xylanase from Trichoderma reesei by Cross-linked Enzyme Aggregates

Abstract—Modern developments in biotechnology have paved the way for extensive use of biocatalysis in industries. Although offering immense potential, industrial application is usually hampered by lack of operational stability, difficulty in recovery as well as limited re-use of the enzyme. These dr...

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Main Authors:	Md Shaarani, S., Md Jahim, J., A. Rahman, R., Md Illias, R.
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/7264/ http://umpir.ump.edu.my/id/eprint/7264/1/fkksa-2014-shalyda-Immobilization_Strategy.pdf

id	ump-7264
recordtype	eprints
spelling	ump-72642015-03-03T09:35:03Z http://umpir.ump.edu.my/id/eprint/7264/ Immobilization Strategy of Recombinant Xylanase from Trichoderma reesei by Cross-linked Enzyme Aggregates Md Shaarani, S. Md Jahim, J. A. Rahman, R. Md Illias, R. TP Chemical technology Abstract—Modern developments in biotechnology have paved the way for extensive use of biocatalysis in industries. Although offering immense potential, industrial application is usually hampered by lack of operational stability, difficulty in recovery as well as limited re-use of the enzyme. These drawbacks however can be overcome by immobilization. Cross-linked enzyme aggregates (CLEAs), a versatile carrier-free immobilization technique is one that is currently capturing global interest. This approach involves precipitating soluble enzyme with an appropriate precipitant and subsequent crosslinking by a crosslinking reagent. Without ineffective carriers, CLEAs offer high enzymatic activity, stability and reduced production cost. This study demonstrated successful CLEA synthesis of recombinant xylanase from Trichoderma reesei using ethanol as aggregating agent and glutaraldehyde (2% (v/v); 100 mM) as crosslinker. Effects of additives including proteic feeder such as bovine serum albumin (BSA) and poly-L-Lysine were investigated to reveal its significance in enhancing the performance of enzyme. Addition of 0.1 mg BSA/U xylanase showed considerable increment in CLEA development with approximately 50% retained activity. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7264/1/fkksa-2014-shalyda-Immobilization_Strategy.pdf Md Shaarani, S. and Md Jahim, J. and A. Rahman, R. and Md Illias, R. (2014) Immobilization Strategy of Recombinant Xylanase from Trichoderma reesei by Cross-linked Enzyme Aggregates. In: International Conference on Bioengineering (ICBE 2014), 19-20 June 2014 , Venice, Italy. .
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Md Shaarani, S. Md Jahim, J. A. Rahman, R. Md Illias, R. Immobilization Strategy of Recombinant Xylanase from Trichoderma reesei by Cross-linked Enzyme Aggregates
description	Abstract—Modern developments in biotechnology have paved the way for extensive use of biocatalysis in industries. Although offering immense potential, industrial application is usually hampered by lack of operational stability, difficulty in recovery as well as limited re-use of the enzyme. These drawbacks however can be overcome by immobilization. Cross-linked enzyme aggregates (CLEAs), a versatile carrier-free immobilization technique is one that is currently capturing global interest. This approach involves precipitating soluble enzyme with an appropriate precipitant and subsequent crosslinking by a crosslinking reagent. Without ineffective carriers, CLEAs offer high enzymatic activity, stability and reduced production cost. This study demonstrated successful CLEA synthesis of recombinant xylanase from Trichoderma reesei using ethanol as aggregating agent and glutaraldehyde (2% (v/v); 100 mM) as crosslinker. Effects of additives including proteic feeder such as bovine serum albumin (BSA) and poly-L-Lysine were investigated to reveal its significance in enhancing the performance of enzyme. Addition of 0.1 mg BSA/U xylanase showed considerable increment in CLEA development with approximately 50% retained activity.
format	Conference or Workshop Item
author	Md Shaarani, S. Md Jahim, J. A. Rahman, R. Md Illias, R.
author_facet	Md Shaarani, S. Md Jahim, J. A. Rahman, R. Md Illias, R.
author_sort	Md Shaarani, S.
title	Immobilization Strategy of Recombinant Xylanase from Trichoderma reesei by Cross-linked Enzyme Aggregates
title_short	Immobilization Strategy of Recombinant Xylanase from Trichoderma reesei by Cross-linked Enzyme Aggregates
title_full	Immobilization Strategy of Recombinant Xylanase from Trichoderma reesei by Cross-linked Enzyme Aggregates
title_fullStr	Immobilization Strategy of Recombinant Xylanase from Trichoderma reesei by Cross-linked Enzyme Aggregates
title_full_unstemmed	Immobilization Strategy of Recombinant Xylanase from Trichoderma reesei by Cross-linked Enzyme Aggregates
title_sort	immobilization strategy of recombinant xylanase from trichoderma reesei by cross-linked enzyme aggregates
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/7264/ http://umpir.ump.edu.my/id/eprint/7264/1/fkksa-2014-shalyda-Immobilization_Strategy.pdf
first_indexed	2023-09-18T22:03:50Z
last_indexed	2023-09-18T22:03:50Z
_version_	1777414575598075904

Immobilization Strategy of Recombinant Xylanase from Trichoderma reesei by Cross-linked Enzyme Aggregates

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