Inoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ)

Polyhydroxybutyrate (PHB) is a biopolymer plastic as intracellular product of bacteria which can be degradable naturally. The purpose of this experiment is to scale up the production of PHB biopolymer by Cupriavidus Necator using oil palm frond juice (OPFJ) as carbon source. The process will involve...

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Main Author:	Nahdiyyah, Rosslan
Format:	Undergraduates Project Papers
Language:	English
Published:	2013
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/7234/ http://umpir.ump.edu.my/id/eprint/7234/ http://umpir.ump.edu.my/id/eprint/7234/1/Inoculum_development_in_2L_stirred_tank_fermenter_for_production_of_PHB_biopolymer.pdf

id	ump-7234
recordtype	eprints
spelling	ump-72342015-03-03T09:34:54Z http://umpir.ump.edu.my/id/eprint/7234/ Inoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ) Nahdiyyah, Rosslan TP Chemical technology Polyhydroxybutyrate (PHB) is a biopolymer plastic as intracellular product of bacteria which can be degradable naturally. The purpose of this experiment is to scale up the production of PHB biopolymer by Cupriavidus Necator using oil palm frond juice (OPFJ) as carbon source. The process will involve fermentation process, the analysis on cell dry weight and concentration of PHB. The fermentation will be done in the 2 L stirred tank fermenter model Sartorius with the combination of 450 ml oil palm frond juice, 150 ml inoculum development 2, and 900 ml mineral salt medium (MSM) by manipulating the agitation speed and fixed the aeration rate at 1.5 L/min. Next, the cell dry weight and PHB will be analyse. From the result of DOT curve, 200 rpm in fermenter shows the same pattern as in 220 rpm in flask. 200 rpm is used in 2L fermentation. Highest PHB yield for fermentation in 2 L was 2.0650 g/L and highest cell dry weight was 5.7 g/L. While for cascade fermentation highest cell dry weight was 9.5 g/L and highest PHB content was 5.42 g/L. 2013 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7234/1/Inoculum_development_in_2L_stirred_tank_fermenter_for_production_of_PHB_biopolymer.pdf Nahdiyyah, Rosslan (2013) Inoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ). Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:76100&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nahdiyyah, Rosslan Inoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ)
description	Polyhydroxybutyrate (PHB) is a biopolymer plastic as intracellular product of bacteria which can be degradable naturally. The purpose of this experiment is to scale up the production of PHB biopolymer by Cupriavidus Necator using oil palm frond juice (OPFJ) as carbon source. The process will involve fermentation process, the analysis on cell dry weight and concentration of PHB. The fermentation will be done in the 2 L stirred tank fermenter model Sartorius with the combination of 450 ml oil palm frond juice, 150 ml inoculum development 2, and 900 ml mineral salt medium (MSM) by manipulating the agitation speed and fixed the aeration rate at 1.5 L/min. Next, the cell dry weight and PHB will be analyse. From the result of DOT curve, 200 rpm in fermenter shows the same pattern as in 220 rpm in flask. 200 rpm is used in 2L fermentation. Highest PHB yield for fermentation in 2 L was 2.0650 g/L and highest cell dry weight was 5.7 g/L. While for cascade fermentation highest cell dry weight was 9.5 g/L and highest PHB content was 5.42 g/L.
format	Undergraduates Project Papers
author	Nahdiyyah, Rosslan
author_facet	Nahdiyyah, Rosslan
author_sort	Nahdiyyah, Rosslan
title	Inoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ)
title_short	Inoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ)
title_full	Inoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ)
title_fullStr	Inoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ)
title_full_unstemmed	Inoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ)
title_sort	inoculum development in 2l stirred tank fermenter for production of phb biopolymer in a 20 l stirred tank fermentation on a medium of oil palm frond juice (opfj)
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/7234/ http://umpir.ump.edu.my/id/eprint/7234/ http://umpir.ump.edu.my/id/eprint/7234/1/Inoculum_development_in_2L_stirred_tank_fermenter_for_production_of_PHB_biopolymer.pdf
first_indexed	2023-09-18T22:03:46Z
last_indexed	2023-09-18T22:03:46Z
_version_	1777414571359731712

Inoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ)

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