Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography

Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography

In dairy industry, whey protein fractionation is an important process that requires an effective method to separate valuable proteins in bovine whey protein. Bovine whey protein can be divided into two components which are major protein and minor proteins. The major protein contains approximately 50...

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Main Author: Rabiatul Adawiyah, Sharom
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
TX Home economics
Online Access:http://umpir.ump.edu.my/id/eprint/7151/
http://umpir.ump.edu.my/id/eprint/7151/
http://umpir.ump.edu.my/id/eprint/7151/1/Whey_protein_fractionation_using_Q-Sepharose.pdf
id ump-7151
recordtype eprints
spelling ump-71512015-03-03T09:34:31Z http://umpir.ump.edu.my/id/eprint/7151/ Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography Rabiatul Adawiyah, Sharom TX Home economics In dairy industry, whey protein fractionation is an important process that requires an effective method to separate valuable proteins in bovine whey protein. Bovine whey protein can be divided into two components which are major protein and minor proteins. The major protein contains approximately 50% of β-lactoglobulin (β-Lg), 20% of α-lactalbumin (α-Lac), 10% of bovine serum albumin (BSA) and 10% of immunoglobulin (Ig). Cation exchange and anion exchange chromatography is one of advance separation techniques that can fractionate bovine whey protein. All negatively charged proteins in whey were bound simultaneously to an anion exchange column (Q-Sepharose), while all positively charged proteins in whey were bound to a cation exchange column (SP-Sepharose). Whey protein and buffer solution (pH 4 - pH 10) were prepared before run the experiment. The method is based on the use of an ionic column and salt gradient elution buffer (Buffer solution plus 1 M NaCl). The collected fractions were analyzing using SDS-PAGE to determine which fractions that contain protein component. By using UPLC the concentration of protein exists in the fraction had been determined. Protein fractionation at pH 7 gives the best result with highest concentration of protein recover by using Q-Sepharose. While, by using SP-Sepharose show pure β-Lg can be fractionate at pH 5. 2013 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7151/1/Whey_protein_fractionation_using_Q-Sepharose.pdf Rabiatul Adawiyah, Sharom (2013) Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:76023&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TX Home economics
spellingShingle TX Home economics
Rabiatul Adawiyah, Sharom
Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography
description In dairy industry, whey protein fractionation is an important process that requires an effective method to separate valuable proteins in bovine whey protein. Bovine whey protein can be divided into two components which are major protein and minor proteins. The major protein contains approximately 50% of β-lactoglobulin (β-Lg), 20% of α-lactalbumin (α-Lac), 10% of bovine serum albumin (BSA) and 10% of immunoglobulin (Ig). Cation exchange and anion exchange chromatography is one of advance separation techniques that can fractionate bovine whey protein. All negatively charged proteins in whey were bound simultaneously to an anion exchange column (Q-Sepharose), while all positively charged proteins in whey were bound to a cation exchange column (SP-Sepharose). Whey protein and buffer solution (pH 4 - pH 10) were prepared before run the experiment. The method is based on the use of an ionic column and salt gradient elution buffer (Buffer solution plus 1 M NaCl). The collected fractions were analyzing using SDS-PAGE to determine which fractions that contain protein component. By using UPLC the concentration of protein exists in the fraction had been determined. Protein fractionation at pH 7 gives the best result with highest concentration of protein recover by using Q-Sepharose. While, by using SP-Sepharose show pure β-Lg can be fractionate at pH 5.
format Undergraduates Project Papers
author Rabiatul Adawiyah, Sharom
author_facet Rabiatul Adawiyah, Sharom
author_sort Rabiatul Adawiyah, Sharom
title Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography
title_short Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography
title_full Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography
title_fullStr Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography
title_full_unstemmed Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography
title_sort whey protein fractionation using q-sepharose anion exchange chromatography and sp-sepharose cation exchange chromatography
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/7151/
http://umpir.ump.edu.my/id/eprint/7151/
http://umpir.ump.edu.my/id/eprint/7151/1/Whey_protein_fractionation_using_Q-Sepharose.pdf
first_indexed 2023-09-18T22:03:34Z
last_indexed 2023-09-18T22:03:34Z
_version_ 1777414559171084288

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