Extraction of phenolic compounds and flavonoids compounds from Orthosiphon Stamineus

Extraction of phenolic compounds and flavonoids compounds from Orthosiphon Stamineus

Phenolic and flavonoids compounds present in Orthosiphon stamineus (OS) exhibit high antioxidant capacity. This research was conducted to compare ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE) with maceration and investigate effects of solvents (70%-30% v/v methanol-wat...

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Main Author: Zhu, Lau Mei
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
QK Botany
Online Access:http://umpir.ump.edu.my/id/eprint/7146/
http://umpir.ump.edu.my/id/eprint/7146/
http://umpir.ump.edu.my/id/eprint/7146/1/Extraction_of_phenolic_compounds.pdf
id ump-7146
recordtype eprints
spelling ump-71462015-03-03T09:34:30Z http://umpir.ump.edu.my/id/eprint/7146/ Extraction of phenolic compounds and flavonoids compounds from Orthosiphon Stamineus Zhu, Lau Mei QK Botany Phenolic and flavonoids compounds present in Orthosiphon stamineus (OS) exhibit high antioxidant capacity. This research was conducted to compare ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE) with maceration and investigate effects of solvents (70%-30% v/v methanol-water, 70%-30% v/v propanol-water, 50%-50% v/v methanol-water, 50%-50% v/v propanol-water, water, methanol and propanol), extraction time, microwave power and extraction temperature on Rosmarinic acid and Sinensetin extraction yields from OS dried powder. The extraction yields were analyzed by using Ultra Performance Liquid Chromatography (UPLC) and were compared to standard calibration curve with their retention times. Results from Total Phenolic Content (TPC) were used to support UPLC result. By UAE, the highest yield for Rosmarinic acid could be obtained with following conditions: 70%-30% v/v Methanol-water as solvent with extraction time of 90 minutes at 60 ̊C. The highest yield for Rosmarinic acid that could be obtained by using MAE was with following conditions: 70%-30% v/v Methanol-water with extraction time of 120 s at 450 W. By UAE, the highest yield for Sinensetin could be obtained with following conditions: propanol as solvent with extraction time of 90 minutes at 60 ̊C. Meanwhile, the highest yield for Rosmarinic acid that could be obtained by using MAE was with following conditions, 70%-30% v/v Methanol-water with extraction time of 120 s at 450 W. UAE and MAE can replace maceration in extraction of phenolic compounds and flavonoids compounds since UAE and MAE had higher yields than maceration in shorter time. Recommendations suggested in order to obtain optimization of this research were to study more effects or parameters in both UAE and MAE. 2013 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7146/1/Extraction_of_phenolic_compounds.pdf Zhu, Lau Mei (2013) Extraction of phenolic compounds and flavonoids compounds from Orthosiphon Stamineus. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:76026&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QK Botany
spellingShingle QK Botany
Zhu, Lau Mei
Extraction of phenolic compounds and flavonoids compounds from Orthosiphon Stamineus
description Phenolic and flavonoids compounds present in Orthosiphon stamineus (OS) exhibit high antioxidant capacity. This research was conducted to compare ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE) with maceration and investigate effects of solvents (70%-30% v/v methanol-water, 70%-30% v/v propanol-water, 50%-50% v/v methanol-water, 50%-50% v/v propanol-water, water, methanol and propanol), extraction time, microwave power and extraction temperature on Rosmarinic acid and Sinensetin extraction yields from OS dried powder. The extraction yields were analyzed by using Ultra Performance Liquid Chromatography (UPLC) and were compared to standard calibration curve with their retention times. Results from Total Phenolic Content (TPC) were used to support UPLC result. By UAE, the highest yield for Rosmarinic acid could be obtained with following conditions: 70%-30% v/v Methanol-water as solvent with extraction time of 90 minutes at 60 ̊C. The highest yield for Rosmarinic acid that could be obtained by using MAE was with following conditions: 70%-30% v/v Methanol-water with extraction time of 120 s at 450 W. By UAE, the highest yield for Sinensetin could be obtained with following conditions: propanol as solvent with extraction time of 90 minutes at 60 ̊C. Meanwhile, the highest yield for Rosmarinic acid that could be obtained by using MAE was with following conditions, 70%-30% v/v Methanol-water with extraction time of 120 s at 450 W. UAE and MAE can replace maceration in extraction of phenolic compounds and flavonoids compounds since UAE and MAE had higher yields than maceration in shorter time. Recommendations suggested in order to obtain optimization of this research were to study more effects or parameters in both UAE and MAE.
format Undergraduates Project Papers
author Zhu, Lau Mei
author_facet Zhu, Lau Mei
author_sort Zhu, Lau Mei
title Extraction of phenolic compounds and flavonoids compounds from Orthosiphon Stamineus
title_short Extraction of phenolic compounds and flavonoids compounds from Orthosiphon Stamineus
title_full Extraction of phenolic compounds and flavonoids compounds from Orthosiphon Stamineus
title_fullStr Extraction of phenolic compounds and flavonoids compounds from Orthosiphon Stamineus
title_full_unstemmed Extraction of phenolic compounds and flavonoids compounds from Orthosiphon Stamineus
title_sort extraction of phenolic compounds and flavonoids compounds from orthosiphon stamineus
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/7146/
http://umpir.ump.edu.my/id/eprint/7146/
http://umpir.ump.edu.my/id/eprint/7146/1/Extraction_of_phenolic_compounds.pdf
first_indexed 2023-09-18T22:03:33Z
last_indexed 2023-09-18T22:03:33Z
_version_ 1777414558464344064

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