Scale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor

This study is about the scale up of biopolymer fermentation from 500 mL shake flasks to 2L fermentor. The purpose of this project is to determine air flow rate at 2L fermentor which gives the same kLa and kap as produced in shake flasks. The rationale of using the kLa value is to ensure a certain ma...

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Main Author:	Aimi Salma, Awalludin
Format:	Undergraduates Project Papers
Language:	English
Published:	2009
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/696/ http://umpir.ump.edu.my/id/eprint/696/1/Aimi_Salma_Awalludin.pdf

id	ump-696
recordtype	eprints
spelling	ump-6962015-03-03T07:46:27Z http://umpir.ump.edu.my/id/eprint/696/ Scale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor Aimi Salma, Awalludin TP Chemical technology This study is about the scale up of biopolymer fermentation from 500 mL shake flasks to 2L fermentor. The purpose of this project is to determine air flow rate at 2L fermentor which gives the same kLa and kap as produced in shake flasks. The rationale of using the kLa value is to ensure a certain mass transfer capability in order to cope with the oxygen demand of the culture, thus being an important scale-up factor. Both values were derived by fitting the dissolved oxygen tension (DOT) versus time data into The Fibonacci Min Search (Fminsearch) method. Two values, which are 0.2960 for volumetric mass transfer coefficient for oxygen (kLa) and 0.0220 for the electrode mass transfer coefficient (kap) were obtained at small scale. These values are required to be duplicated in the larger scale. It was done by using a fixed agitation rate of 200 rpm and a manipulated aeration rate which is 1L/min,1.5L/min,2L/min and 1.75L/min. The most comparable kLa and kap values obtained from the trial are at 1.75L/min. This aeration rate will be used in the 2L fermentor in order to investigate the production of poly-β-hydroxybutyrate (PHB). Fermentation is run at both scales to compare the glucose, biomass and PHB profile. From the experiment and calculation, the maximum concentration of PHB is achieve at the 36th hours, which is 1.415g/L for 500 mL shake flasks, and 2.17g/L for 2L fermentor. The cell dry mass obtained at the optimum harvesting time is 6.065 g/L for both scales.-Author- 2009-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/696/1/Aimi_Salma_Awalludin.pdf Aimi Salma, Awalludin (2009) Scale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Aimi Salma, Awalludin Scale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor
description	This study is about the scale up of biopolymer fermentation from 500 mL shake flasks to 2L fermentor. The purpose of this project is to determine air flow rate at 2L fermentor which gives the same kLa and kap as produced in shake flasks. The rationale of using the kLa value is to ensure a certain mass transfer capability in order to cope with the oxygen demand of the culture, thus being an important scale-up factor. Both values were derived by fitting the dissolved oxygen tension (DOT) versus time data into The Fibonacci Min Search (Fminsearch) method. Two values, which are 0.2960 for volumetric mass transfer coefficient for oxygen (kLa) and 0.0220 for the electrode mass transfer coefficient (kap) were obtained at small scale. These values are required to be duplicated in the larger scale. It was done by using a fixed agitation rate of 200 rpm and a manipulated aeration rate which is 1L/min,1.5L/min,2L/min and 1.75L/min. The most comparable kLa and kap values obtained from the trial are at 1.75L/min. This aeration rate will be used in the 2L fermentor in order to investigate the production of poly-β-hydroxybutyrate (PHB). Fermentation is run at both scales to compare the glucose, biomass and PHB profile. From the experiment and calculation, the maximum concentration of PHB is achieve at the 36th hours, which is 1.415g/L for 500 mL shake flasks, and 2.17g/L for 2L fermentor. The cell dry mass obtained at the optimum harvesting time is 6.065 g/L for both scales.-Author-
format	Undergraduates Project Papers
author	Aimi Salma, Awalludin
author_facet	Aimi Salma, Awalludin
author_sort	Aimi Salma, Awalludin
title	Scale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor
title_short	Scale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor
title_full	Scale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor
title_fullStr	Scale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor
title_full_unstemmed	Scale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor
title_sort	scale up of biopolymer (phb) fermentation from 500ml shake flasks to 2l stirred tank fermentor
publishDate	2009
url	http://umpir.ump.edu.my/id/eprint/696/ http://umpir.ump.edu.my/id/eprint/696/1/Aimi_Salma_Awalludin.pdf
first_indexed	2023-09-18T21:53:10Z
last_indexed	2023-09-18T21:53:10Z
_version_	1777413904990732288

Scale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor

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