Lignin degradation of banana stem wastes using aerobic Bacillus cereus
The delignification process of banana stem wastes using Bacillus cereus was studied. This study is divided into three parts which are determination growth curve of Bacillus cereus, batch fermentation and lignin analysis using Klason method. Delignification process is functioning to degrade lignin fr...
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| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2009
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| Online Access: | http://umpir.ump.edu.my/id/eprint/695/ http://umpir.ump.edu.my/id/eprint/695/ http://umpir.ump.edu.my/id/eprint/695/1/Shajaratuldur_Ismail.pdf |
| Summary: | The delignification process of banana stem wastes using Bacillus cereus was studied. This study is divided into three parts which are determination growth curve of Bacillus cereus, batch fermentation and lignin analysis using Klason method. Delignification process is functioning to degrade lignin from banana stem to get cellulose that can be used as carbon sources. Bacillus cereus is used as microorganisms to degrade lignin from the banana stem wastes. The banana stem contain 35% of cellulose on dry weight basis. The methods are divided into three parts which are preparation of raw material and microorganisms, fermentation process and lignin analysis. Fermentation is carried out aerobically at 30˚C and 37 ˚C, and 250 rpm of agitation speed for 2 days. In this process, Klason method is used to identify the percentage of lignin degraded. Based on the analysis, it shows that Bacillus cereus is able to degrade lignin from banana stem wastes. The optimum temperature for Bacillus cereus is 37°C with the average percentage of lignin degradation is 28.69%. |
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