Optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology

Although, the cellulase enzyme can work with high catalysis under mild condition, however, it does not fulfill the industry requirement. In this study, cellulase obtained from the fermentation of sewage treatment plant (STP) sludge by Trichoderma reesei RUT C-30 was covalently immobilised on functio...

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Main Authors:	Rasha, Mohammed Abd, Nour, A. H.
Format:	Article
Language:	English
Published:	Academic Journals 2012
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/6892/ http://umpir.ump.edu.my/id/eprint/6892/ http://umpir.ump.edu.my/id/eprint/6892/ http://umpir.ump.edu.my/id/eprint/6892/1/Optimisation_of_immobilised_cellulase_onto_carbon.pdf

id	ump-6892
recordtype	eprints
spelling	ump-68922018-01-24T04:30:42Z http://umpir.ump.edu.my/id/eprint/6892/ Optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology Rasha, Mohammed Abd Nour, A. H. QD Chemistry Although, the cellulase enzyme can work with high catalysis under mild condition, however, it does not fulfill the industry requirement. In this study, cellulase obtained from the fermentation of sewage treatment plant (STP) sludge by Trichoderma reesei RUT C-30 was covalently immobilised on functionalised multi-wall carbon nanotubes (MWCNTs). Statistical optimisation using the PlackettBurman design (PBD) method was implemented to identify parameters with significant effects on the process of immobilisation. The results obtained from this PBD showed that three parameters have a significant effect on immobilisation: pH, temperature and N-ethyl-N-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) concentration. Based on our PBD results, these parameters were further optimised using a face-centred central composite design (FCCCD). The resulting optimum conditions for cellulase immobilisation, as determined by FCCCD, were pH 4.5, 30°C and 1 ml of 10mg/ml EDC. The amount of immobilised cellulase was approximately 98% using these optimum conditions. The resulting MWCNT-cellulase composite was further characterized by Fourier transform infrared spectroscopy (FTIR) technique. Academic Journals 2012 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6892/1/Optimisation_of_immobilised_cellulase_onto_carbon.pdf Rasha, Mohammed Abd and Nour, A. H. (2012) Optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology. International Journal of the Physical Sciences, 7 (5). pp. 841-849. ISSN 1992-1950 http://www.academicjournals.org/journal/IJPS/article-abstract/5D2135217110 10.5897/IJPS11.1639
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Rasha, Mohammed Abd Nour, A. H. Optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology
description	Although, the cellulase enzyme can work with high catalysis under mild condition, however, it does not fulfill the industry requirement. In this study, cellulase obtained from the fermentation of sewage treatment plant (STP) sludge by Trichoderma reesei RUT C-30 was covalently immobilised on functionalised multi-wall carbon nanotubes (MWCNTs). Statistical optimisation using the PlackettBurman design (PBD) method was implemented to identify parameters with significant effects on the process of immobilisation. The results obtained from this PBD showed that three parameters have a significant effect on immobilisation: pH, temperature and N-ethyl-N-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) concentration. Based on our PBD results, these parameters were further optimised using a face-centred central composite design (FCCCD). The resulting optimum conditions for cellulase immobilisation, as determined by FCCCD, were pH 4.5, 30°C and 1 ml of 10mg/ml EDC. The amount of immobilised cellulase was approximately 98% using these optimum conditions. The resulting MWCNT-cellulase composite was further characterized by Fourier transform infrared spectroscopy (FTIR) technique.
format	Article
author	Rasha, Mohammed Abd Nour, A. H.
author_facet	Rasha, Mohammed Abd Nour, A. H.
author_sort	Rasha, Mohammed Abd
title	Optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology
title_short	Optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology
title_full	Optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology
title_fullStr	Optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology
title_full_unstemmed	Optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology
title_sort	optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology
publisher	Academic Journals
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/6892/ http://umpir.ump.edu.my/id/eprint/6892/ http://umpir.ump.edu.my/id/eprint/6892/ http://umpir.ump.edu.my/id/eprint/6892/1/Optimisation_of_immobilised_cellulase_onto_carbon.pdf
first_indexed	2023-09-18T22:03:04Z
last_indexed	2023-09-18T22:03:04Z
_version_	1777414527192662016

Optimisation of immobilised cellulase onto carbon nanotubes using response surface methodology

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