Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide

Hepatitis B (Hep B) is a viral infection caused by hepatitis B virus (HBV) that infects the human liver. HBV is bloodborne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide. Approximately 5% of the Malaysian population is infected by HBV....

Full description

Bibliographic Details
Main Authors:	Geraldine Chan, Sue Ching, Willy Wong, Xiu Fa, Chong, Fui Chin
Format:	Article
Language:	English English English
Published:	Penerbit Universiti Teknologi Malaysia 2015
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/6745/ http://umpir.ump.edu.my/id/eprint/6745/ http://umpir.ump.edu.my/id/eprint/6745/ http://umpir.ump.edu.my/id/eprint/6745/1/fkksa-2014-Geraldine-Immobilization_Of_Hepatitis_B.pdf http://umpir.ump.edu.my/id/eprint/6745/2/fkksa-2014-geraldine-posterbiojohor.JPG http://umpir.ump.edu.my/id/eprint/6745/8/fkksa-2015-geraldine-%20Immobilization%20of%20Hepatitis%20B%20Antibody.pdf

id	ump-6745
recordtype	eprints
spelling	ump-67452019-10-17T08:01:49Z http://umpir.ump.edu.my/id/eprint/6745/ Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide Geraldine Chan, Sue Ching Willy Wong, Xiu Fa Chong, Fui Chin TP Chemical technology Hepatitis B (Hep B) is a viral infection caused by hepatitis B virus (HBV) that infects the human liver. HBV is bloodborne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide. Approximately 5% of the Malaysian population is infected by HBV. The treatment to this disease is possible if early detection is done before irreversible liver damage occurs. The presence of Hepatitis B virus surface antigen (HB5Ag) is an evidence of the presence of HBV infections. Presently, the conventional methods used for detection shows weakness in terms of time and efficiency. In this research, we aim to study on the feasibility of reduced graphene oxide (rGO) as the base material for immune biosensor for the detection of HB5Ag. The graphene oxide was prepared via Modified Hummer's Method due to its ability to produce graphene in large quantity. The fabrication of immunosensor was conducted by immobilizing antibody onto the graphene. Nafion-graphene nanosheet was grown and later treated with Thionine solution. Following that, a solution of primary antibodies against Hep B surface antigen (anti-HB5Ag lgG) was incubated on the biosensor. After the immobilization, the antigen was dropped onto the biosensor, followed by incubation of secondary antibodies conjugated to Horseradish Peroxidase (HRP). Upon addition of Tetramethylbenzid me (TMB), the colour of the solution changes to blue. Penerbit Universiti Teknologi Malaysia 2015 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/6745/1/fkksa-2014-Geraldine-Immobilization_Of_Hepatitis_B.pdf pdf en http://umpir.ump.edu.my/id/eprint/6745/2/fkksa-2014-geraldine-posterbiojohor.JPG pdf en http://umpir.ump.edu.my/id/eprint/6745/8/fkksa-2015-geraldine-%20Immobilization%20of%20Hepatitis%20B%20Antibody.pdf Geraldine Chan, Sue Ching and Willy Wong, Xiu Fa and Chong, Fui Chin (2015) Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide. Jurnal Teknologi (Sciences and Engineering), 77 (31). pp. 25-29. ISSN 0127-9696 (print); 2180-3722 (online) https://doi.org/10.11113/jt.v77.6895 doi: 10.11113/jt.v77.6895
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Geraldine Chan, Sue Ching Willy Wong, Xiu Fa Chong, Fui Chin Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
description	Hepatitis B (Hep B) is a viral infection caused by hepatitis B virus (HBV) that infects the human liver. HBV is bloodborne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide. Approximately 5% of the Malaysian population is infected by HBV. The treatment to this disease is possible if early detection is done before irreversible liver damage occurs. The presence of Hepatitis B virus surface antigen (HB5Ag) is an evidence of the presence of HBV infections. Presently, the conventional methods used for detection shows weakness in terms of time and efficiency. In this research, we aim to study on the feasibility of reduced graphene oxide (rGO) as the base material for immune biosensor for the detection of HB5Ag. The graphene oxide was prepared via Modified Hummer's Method due to its ability to produce graphene in large quantity. The fabrication of immunosensor was conducted by immobilizing antibody onto the graphene. Nafion-graphene nanosheet was grown and later treated with Thionine solution. Following that, a solution of primary antibodies against Hep B surface antigen (anti-HB5Ag lgG) was incubated on the biosensor. After the immobilization, the antigen was dropped onto the biosensor, followed by incubation of secondary antibodies conjugated to Horseradish Peroxidase (HRP). Upon addition of Tetramethylbenzid me (TMB), the colour of the solution changes to blue.
format	Article
author	Geraldine Chan, Sue Ching Willy Wong, Xiu Fa Chong, Fui Chin
author_facet	Geraldine Chan, Sue Ching Willy Wong, Xiu Fa Chong, Fui Chin
author_sort	Geraldine Chan, Sue Ching
title	Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
title_short	Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
title_full	Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
title_fullStr	Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
title_full_unstemmed	Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
title_sort	immobilization of hepatitis b antibody onto the reduced graphene oxide
publisher	Penerbit Universiti Teknologi Malaysia
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/6745/ http://umpir.ump.edu.my/id/eprint/6745/ http://umpir.ump.edu.my/id/eprint/6745/ http://umpir.ump.edu.my/id/eprint/6745/1/fkksa-2014-Geraldine-Immobilization_Of_Hepatitis_B.pdf http://umpir.ump.edu.my/id/eprint/6745/2/fkksa-2014-geraldine-posterbiojohor.JPG http://umpir.ump.edu.my/id/eprint/6745/8/fkksa-2015-geraldine-%20Immobilization%20of%20Hepatitis%20B%20Antibody.pdf
first_indexed	2023-09-18T22:02:46Z
last_indexed	2023-09-18T22:02:46Z
_version_	1777414509167640576

Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide

Similar Items