Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study

As the gradual up-growing trend of industrialization and urbanization leading steady increment of demand of energy; eco-friendly, bio degradable, cost competitive and promising source of energy with high sustainability is toughly needed for the new era of modern world. Hydrolysis of cellulose by cel...

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Main Authors: Saha, Pradip, Khan, Maksudur R., Deb, T. K., Majumdar, S., Alam, F., Sarker, N. C.
Format: Article
Language:English
English
Published: Bangladesh Journals OnLine (BanglaJOL) 2012
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/6400/
http://umpir.ump.edu.my/id/eprint/6400/
http://umpir.ump.edu.my/id/eprint/6400/
http://umpir.ump.edu.my/id/eprint/6400/1/Enzymatic_Hydrolysis_of_Rice_Straw_to_Fermentable_Sugar-_Kinetic_Study.pdf
http://umpir.ump.edu.my/id/eprint/6400/4/Enzymatic%20Hydrolysis.pdf
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spelling ump-64002018-01-16T03:10:59Z http://umpir.ump.edu.my/id/eprint/6400/ Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study Saha, Pradip Khan, Maksudur R. Deb, T. K. Majumdar, S. Alam, F. Sarker, N. C. TP Chemical technology As the gradual up-growing trend of industrialization and urbanization leading steady increment of demand of energy; eco-friendly, bio degradable, cost competitive and promising source of energy with high sustainability is toughly needed for the new era of modern world. Hydrolysis of cellulose by cellulase enzymes is a vital candidate for this option. It is a solid-liquid heterogeneous reaction; strongly affected by the non-reaction resistances caused most notably by the crystalline structure; reaction environment parameters as temperature, pH, characteristics of enzyme, cell & substrate loading and hence must have to be defined for specific enzyme-substrate amalgamation. In this present investigation, glucose was produced from rice straw using cellulytic enzyme pseudomonas sp., isolated from municipal solid waste. Glucose yield was found to increase as the rice straw particle size decreased from 0.5 mm to 45 μm, while the optimal temperature and pH were found within the range of 30°C and 7.0 respectively. The concentration and rate of glucose production was observed to depend on pretreatment of rice straw, substrate concentration and enzyme loading. A kinetic model rate expression has been developed for such a process based on the Michaelis – Mentens and Line weaver–Burk approach. Comparison between the experimental data and those predicted from the rate model indicate good agreement with a mean absolute deviation of about 0.304916. Bangladesh Journals OnLine (BanglaJOL) 2012 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6400/1/Enzymatic_Hydrolysis_of_Rice_Straw_to_Fermentable_Sugar-_Kinetic_Study.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/6400/4/Enzymatic%20Hydrolysis.pdf Saha, Pradip and Khan, Maksudur R. and Deb, T. K. and Majumdar, S. and Alam, F. and Sarker, N. C. (2012) Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study. Journal of Chemical Engineering, 27 (2). pp. 20-24. ISSN 2221-7436 http://dx.doi.org/10.3329/jce.v27i2.17778 DOI: 10.3329/jce.v27i2.17778
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Saha, Pradip
Khan, Maksudur R.
Deb, T. K.
Majumdar, S.
Alam, F.
Sarker, N. C.
Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study
description As the gradual up-growing trend of industrialization and urbanization leading steady increment of demand of energy; eco-friendly, bio degradable, cost competitive and promising source of energy with high sustainability is toughly needed for the new era of modern world. Hydrolysis of cellulose by cellulase enzymes is a vital candidate for this option. It is a solid-liquid heterogeneous reaction; strongly affected by the non-reaction resistances caused most notably by the crystalline structure; reaction environment parameters as temperature, pH, characteristics of enzyme, cell & substrate loading and hence must have to be defined for specific enzyme-substrate amalgamation. In this present investigation, glucose was produced from rice straw using cellulytic enzyme pseudomonas sp., isolated from municipal solid waste. Glucose yield was found to increase as the rice straw particle size decreased from 0.5 mm to 45 μm, while the optimal temperature and pH were found within the range of 30°C and 7.0 respectively. The concentration and rate of glucose production was observed to depend on pretreatment of rice straw, substrate concentration and enzyme loading. A kinetic model rate expression has been developed for such a process based on the Michaelis – Mentens and Line weaver–Burk approach. Comparison between the experimental data and those predicted from the rate model indicate good agreement with a mean absolute deviation of about 0.304916.
format Article
author Saha, Pradip
Khan, Maksudur R.
Deb, T. K.
Majumdar, S.
Alam, F.
Sarker, N. C.
author_facet Saha, Pradip
Khan, Maksudur R.
Deb, T. K.
Majumdar, S.
Alam, F.
Sarker, N. C.
author_sort Saha, Pradip
title Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study
title_short Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study
title_full Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study
title_fullStr Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study
title_full_unstemmed Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study
title_sort enzymatic hydrolysis of rice straw to fermentable sugar: kinetic study
publisher Bangladesh Journals OnLine (BanglaJOL)
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/6400/
http://umpir.ump.edu.my/id/eprint/6400/
http://umpir.ump.edu.my/id/eprint/6400/
http://umpir.ump.edu.my/id/eprint/6400/1/Enzymatic_Hydrolysis_of_Rice_Straw_to_Fermentable_Sugar-_Kinetic_Study.pdf
http://umpir.ump.edu.my/id/eprint/6400/4/Enzymatic%20Hydrolysis.pdf
first_indexed 2023-09-18T22:02:07Z
last_indexed 2023-09-18T22:02:07Z
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