Application of calcined kaolin as support for the immobilization of lipase from candida rugosa
Lipase from Candida rugosa was immobilized onto calcined-kaolin by physical adsorption method. Lipase offers wide ranges of applications; where as the ability of lipase to act in both organic and inorganic solvents making it more valuable in term of application. However the major concern still wil...
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| Format: | Undergraduates Project Papers |
| Language: | English |
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2008
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| Online Access: | http://umpir.ump.edu.my/id/eprint/626/ http://umpir.ump.edu.my/id/eprint/626/ http://umpir.ump.edu.my/id/eprint/626/1/Shahrul_Nazmi_Othman.PDF |
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ump-626 |
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ump-6262015-03-03T06:04:35Z http://umpir.ump.edu.my/id/eprint/626/ Application of calcined kaolin as support for the immobilization of lipase from candida rugosa Shahrul Nazmi, Othman QP Physiology Lipase from Candida rugosa was immobilized onto calcined-kaolin by physical adsorption method. Lipase offers wide ranges of applications; where as the ability of lipase to act in both organic and inorganic solvents making it more valuable in term of application. However the major concern still will be the cost of lipase, the availability of lipase and reusability of lipase. In order to curb the problems, immobilization of lipase is becoming normal practices. Kaolin is natural substance that obtained from clay. With abilities to act as good as ceramic material; (no toxicity, ability to withstand extreme heat and pressure) it is believed that kaolin has tremendous potential to be utilized as solid support material in both laboratory scale and industrial scale. The study was focus on the preparation of treated kaolin to utilize natural resources instead of inorganic substances. The objectives of this study are to study, amount of lipase attach to kaolin as support material in form of immobilized lipase, to record performance of immobilized lipase with the effect of temperature and effect of pH. The last objective is to compare enzyme activity between free lipase and immobilized lipase. It is determined that percentage of protein loading is 68% (relative amount of lipase attached to solid support material). 2008-05 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/626/1/Shahrul_Nazmi_Othman.PDF Shahrul Nazmi, Othman (2008) Application of calcined kaolin as support for the immobilization of lipase from candida rugosa. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:31051&theme=UMP2 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Malaysia Pahang |
| building |
UMP Institutional Repository |
| collection |
Online Access |
| language |
English |
| topic |
QP Physiology |
| spellingShingle |
QP Physiology Shahrul Nazmi, Othman Application of calcined kaolin as support for the immobilization of lipase from candida rugosa |
| description |
Lipase from Candida rugosa was immobilized onto calcined-kaolin by physical adsorption method. Lipase offers wide ranges of applications; where as the ability of lipase to act in both organic and inorganic solvents making it more valuable in term of application. However the major concern still will be the cost of lipase, the availability of lipase and reusability of lipase. In order to curb the problems, immobilization of lipase is becoming normal practices. Kaolin is natural substance that obtained from clay. With abilities to act as good as ceramic material; (no toxicity, ability to withstand extreme heat and pressure) it is believed that kaolin has tremendous potential to be utilized as solid support material in both laboratory scale and industrial scale. The study was focus on the preparation of treated kaolin to utilize natural resources instead of inorganic substances. The objectives of this study are to study, amount of lipase attach to kaolin as support material in form of immobilized lipase, to record performance of immobilized lipase with the effect of temperature and effect of pH. The last objective is to compare enzyme activity between free lipase and immobilized lipase. It is determined that percentage of protein loading is 68% (relative amount of lipase attached to solid support material). |
| format |
Undergraduates Project Papers |
| author |
Shahrul Nazmi, Othman |
| author_facet |
Shahrul Nazmi, Othman |
| author_sort |
Shahrul Nazmi, Othman |
| title |
Application of calcined kaolin as support for the immobilization of lipase from candida rugosa |
| title_short |
Application of calcined kaolin as support for the immobilization of lipase from candida rugosa |
| title_full |
Application of calcined kaolin as support for the immobilization of lipase from candida rugosa |
| title_fullStr |
Application of calcined kaolin as support for the immobilization of lipase from candida rugosa |
| title_full_unstemmed |
Application of calcined kaolin as support for the immobilization of lipase from candida rugosa |
| title_sort |
application of calcined kaolin as support for the immobilization of lipase from candida rugosa |
| publishDate |
2008 |
| url |
http://umpir.ump.edu.my/id/eprint/626/ http://umpir.ump.edu.my/id/eprint/626/ http://umpir.ump.edu.my/id/eprint/626/1/Shahrul_Nazmi_Othman.PDF |
| first_indexed |
2023-09-18T21:53:03Z |
| last_indexed |
2023-09-18T21:53:03Z |
| _version_ |
1777413897540599808 |