Fatty Acid Composition and Antibacterial Activity of Neem (Azadirachta indica) Seed Oil

Pneumonia and skin diseases are some types of severe infections occur worldwide due to pathogenic bacteria such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). These bacteria’s do contribute towards other infections in human and thus lead towards unhealthy life. The aim of this...

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Main Authors:	Jessinta, Sandanasamy, Azhari, H. Nour, Saiful Nizam, Tajuddin, Nour, A. H.
Format:	Article
Language:	English
Published:	Bentham Open 2013
Subjects:	Q Science (General) TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/6151/ http://umpir.ump.edu.my/id/eprint/6151/ http://umpir.ump.edu.my/id/eprint/6151/ http://umpir.ump.edu.my/id/eprint/6151/1/Fatty_Acid_Composition_and_Antibacterial_Activity_of_Neem_%28Azadirachta.pdf

id	ump-6151
recordtype	eprints
spelling	ump-61512018-01-24T02:32:41Z http://umpir.ump.edu.my/id/eprint/6151/ Fatty Acid Composition and Antibacterial Activity of Neem (Azadirachta indica) Seed Oil Jessinta, Sandanasamy Azhari, H. Nour Saiful Nizam, Tajuddin Nour, A. H. Q Science (General) TP Chemical technology Pneumonia and skin diseases are some types of severe infections occur worldwide due to pathogenic bacteria such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). These bacteria’s do contribute towards other infections in human and thus lead towards unhealthy life. The aim of this study was to determine the fatty acid composition and to investigate the toxic effect of seed oil of Neem, genus Azadirachta of Azadirachta indica (Family: Meliaceae) against two multi-drug resistant bacteria, namely; E. coli and S. aureus. The oil was extracted for 6 h through soxhlet method using hexane as solvent. The fatty acid composition was analyzed via Gas Chromatography-Mass Spectrometry (GC-MS). The composition resulted in detection of 8 fatty acids, whereby the dominant compound is linoleic acid of 34.69% and followed by oleic-, stearic-, palmitic-, arachidic-, behenic-, lignoceric-, and palmiticoleic acid at the percentage of 20.46, 20.42, 18.66, 3.59, 0.80, 0.55, and 0.17%, respectively. The bioassays were conducted through in vitro activity via disc diffusion method with five (5, 20, 50, 80, and 100 mg/mL) different concentrations. Streptomycin and 1% Dimethyl sulfoxide (DMSO) were used as positive and negative controls respectively. The bioassay provided inhibition zone that lies between 8.7 to 11.7 mm and 8.7 to 13.0 mm for E. coli and S. aureus respectively at the concentration of 5 to 100 mg/mL. The antibacterial activity of Neem (A indica) seed oil against these bacteria depends on the correlation of its concentration and thus isolation of bioactive components should be conducted. Bentham Open 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6151/1/Fatty_Acid_Composition_and_Antibacterial_Activity_of_Neem_%28Azadirachta.pdf Jessinta, Sandanasamy and Azhari, H. Nour and Saiful Nizam, Tajuddin and Nour, A. H. (2013) Fatty Acid Composition and Antibacterial Activity of Neem (Azadirachta indica) Seed Oil. The Open Conference Proceedings Journal, 4. pp. 42-48. ISSN 2210-2892 http://dx.doi.org/10.2174/2210289201304020043 DOI: 10.2174/2210289201304020043
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) TP Chemical technology
spellingShingle	Q Science (General) TP Chemical technology Jessinta, Sandanasamy Azhari, H. Nour Saiful Nizam, Tajuddin Nour, A. H. Fatty Acid Composition and Antibacterial Activity of Neem (Azadirachta indica) Seed Oil
description	Pneumonia and skin diseases are some types of severe infections occur worldwide due to pathogenic bacteria such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). These bacteria’s do contribute towards other infections in human and thus lead towards unhealthy life. The aim of this study was to determine the fatty acid composition and to investigate the toxic effect of seed oil of Neem, genus Azadirachta of Azadirachta indica (Family: Meliaceae) against two multi-drug resistant bacteria, namely; E. coli and S. aureus. The oil was extracted for 6 h through soxhlet method using hexane as solvent. The fatty acid composition was analyzed via Gas Chromatography-Mass Spectrometry (GC-MS). The composition resulted in detection of 8 fatty acids, whereby the dominant compound is linoleic acid of 34.69% and followed by oleic-, stearic-, palmitic-, arachidic-, behenic-, lignoceric-, and palmiticoleic acid at the percentage of 20.46, 20.42, 18.66, 3.59, 0.80, 0.55, and 0.17%, respectively. The bioassays were conducted through in vitro activity via disc diffusion method with five (5, 20, 50, 80, and 100 mg/mL) different concentrations. Streptomycin and 1% Dimethyl sulfoxide (DMSO) were used as positive and negative controls respectively. The bioassay provided inhibition zone that lies between 8.7 to 11.7 mm and 8.7 to 13.0 mm for E. coli and S. aureus respectively at the concentration of 5 to 100 mg/mL. The antibacterial activity of Neem (A indica) seed oil against these bacteria depends on the correlation of its concentration and thus isolation of bioactive components should be conducted.
format	Article
author	Jessinta, Sandanasamy Azhari, H. Nour Saiful Nizam, Tajuddin Nour, A. H.
author_facet	Jessinta, Sandanasamy Azhari, H. Nour Saiful Nizam, Tajuddin Nour, A. H.
author_sort	Jessinta, Sandanasamy
title	Fatty Acid Composition and Antibacterial Activity of Neem (Azadirachta indica) Seed Oil
title_short	Fatty Acid Composition and Antibacterial Activity of Neem (Azadirachta indica) Seed Oil
title_full	Fatty Acid Composition and Antibacterial Activity of Neem (Azadirachta indica) Seed Oil
title_fullStr	Fatty Acid Composition and Antibacterial Activity of Neem (Azadirachta indica) Seed Oil
title_full_unstemmed	Fatty Acid Composition and Antibacterial Activity of Neem (Azadirachta indica) Seed Oil
title_sort	fatty acid composition and antibacterial activity of neem (azadirachta indica) seed oil
publisher	Bentham Open
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/6151/ http://umpir.ump.edu.my/id/eprint/6151/ http://umpir.ump.edu.my/id/eprint/6151/ http://umpir.ump.edu.my/id/eprint/6151/1/Fatty_Acid_Composition_and_Antibacterial_Activity_of_Neem_%28Azadirachta.pdf
first_indexed	2023-09-18T22:01:41Z
last_indexed	2023-09-18T22:01:41Z
_version_	1777414440191262720

Fatty Acid Composition and Antibacterial Activity of Neem (Azadirachta indica) Seed Oil

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