Fabrication of Nanofibrous Hydroxyethyl Cellulose/ poly (vinyl alcohol) Scaffolds for Skin Tissue Engineering

In this research we have developed biocompatible nanofibrous mats with hydroxyethyl cellulose (HEC) blended with poly(vinyl alcohol) (PVA) using water as the only solvent. Electrospinning of hydroxyethyl cellulose (HEC) (5 wt%) with poly(vinyl alcohol) (PVA) (15 wt%) at different weight ratios 50:...

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Main Authors:	Jahir Hussain, Fathima Shahitha, Farah Hanani, Zulkifli, Mohammad Syaiful, B. A. R., M. M., Yusoff
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/6020/ http://umpir.ump.edu.my/id/eprint/6020/1/fsti-2014-Fathima_Shahitha-fabrication_of_nanofibrous.pdf http://umpir.ump.edu.my/id/eprint/6020/2/Nanosmat_presentation.pdf

id	ump-6020
recordtype	eprints
spelling	ump-60202018-10-17T04:19:21Z http://umpir.ump.edu.my/id/eprint/6020/ Fabrication of Nanofibrous Hydroxyethyl Cellulose/ poly (vinyl alcohol) Scaffolds for Skin Tissue Engineering Jahir Hussain, Fathima Shahitha Farah Hanani, Zulkifli Mohammad Syaiful, B. A. R. M. M., Yusoff QD Chemistry In this research we have developed biocompatible nanofibrous mats with hydroxyethyl cellulose (HEC) blended with poly(vinyl alcohol) (PVA) using water as the only solvent. Electrospinning of hydroxyethyl cellulose (HEC) (5 wt%) with poly(vinyl alcohol) (PVA) (15 wt%) at different weight ratios 50:50, 40:60 and 30:70 was carried out and evaluated for optimal tissue engineering scaffolds. The nanofibrous mats were cross-linked using glutaraldehyde and characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). SEM images showed that the mean diameters of blend nanofibers were gradually increased from 241±17.03 to 320±27.17 nm with the increased of PVA content. Thermal characterization indicated the stability of HEC/PVA nanofibers was increased with the decreased of PVA content. Studies on cell-scaffolds interaction was carried out by culturing human melanoma cells (A375) on HEC/PVA nanofibers by assessing the growth, proliferation and morphologies of cells. The SEM results shows that A375 cells differentiated and spread well on all HEC/PVA nanofibrous scaffolds with globular morphology after 7 days culturing which exhibit excellent biocompatibility and enhancement of cell penetration and growth within nanofibrous mats. The cellular adhesion profiles and stability results promises that these fibrous mats are potential scaffold for skin tissue engineering. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6020/1/fsti-2014-Fathima_Shahitha-fabrication_of_nanofibrous.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/6020/2/Nanosmat_presentation.pdf Jahir Hussain, Fathima Shahitha and Farah Hanani, Zulkifli and Mohammad Syaiful, B. A. R. and M. M., Yusoff (2014) Fabrication of Nanofibrous Hydroxyethyl Cellulose/ poly (vinyl alcohol) Scaffolds for Skin Tissue Engineering. In: Nanosmat-USA Conference 2014, 19-22 May 2014 , Houston, Texas, USA. . (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Jahir Hussain, Fathima Shahitha Farah Hanani, Zulkifli Mohammad Syaiful, B. A. R. M. M., Yusoff Fabrication of Nanofibrous Hydroxyethyl Cellulose/ poly (vinyl alcohol) Scaffolds for Skin Tissue Engineering
description	In this research we have developed biocompatible nanofibrous mats with hydroxyethyl cellulose (HEC) blended with poly(vinyl alcohol) (PVA) using water as the only solvent. Electrospinning of hydroxyethyl cellulose (HEC) (5 wt%) with poly(vinyl alcohol) (PVA) (15 wt%) at different weight ratios 50:50, 40:60 and 30:70 was carried out and evaluated for optimal tissue engineering scaffolds. The nanofibrous mats were cross-linked using glutaraldehyde and characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). SEM images showed that the mean diameters of blend nanofibers were gradually increased from 241±17.03 to 320±27.17 nm with the increased of PVA content. Thermal characterization indicated the stability of HEC/PVA nanofibers was increased with the decreased of PVA content. Studies on cell-scaffolds interaction was carried out by culturing human melanoma cells (A375) on HEC/PVA nanofibers by assessing the growth, proliferation and morphologies of cells. The SEM results shows that A375 cells differentiated and spread well on all HEC/PVA nanofibrous scaffolds with globular morphology after 7 days culturing which exhibit excellent biocompatibility and enhancement of cell penetration and growth within nanofibrous mats. The cellular adhesion profiles and stability results promises that these fibrous mats are potential scaffold for skin tissue engineering.
format	Conference or Workshop Item
author	Jahir Hussain, Fathima Shahitha Farah Hanani, Zulkifli Mohammad Syaiful, B. A. R. M. M., Yusoff
author_facet	Jahir Hussain, Fathima Shahitha Farah Hanani, Zulkifli Mohammad Syaiful, B. A. R. M. M., Yusoff
author_sort	Jahir Hussain, Fathima Shahitha
title	Fabrication of Nanofibrous Hydroxyethyl Cellulose/ poly (vinyl alcohol) Scaffolds for Skin Tissue Engineering
title_short	Fabrication of Nanofibrous Hydroxyethyl Cellulose/ poly (vinyl alcohol) Scaffolds for Skin Tissue Engineering
title_full	Fabrication of Nanofibrous Hydroxyethyl Cellulose/ poly (vinyl alcohol) Scaffolds for Skin Tissue Engineering
title_fullStr	Fabrication of Nanofibrous Hydroxyethyl Cellulose/ poly (vinyl alcohol) Scaffolds for Skin Tissue Engineering
title_full_unstemmed	Fabrication of Nanofibrous Hydroxyethyl Cellulose/ poly (vinyl alcohol) Scaffolds for Skin Tissue Engineering
title_sort	fabrication of nanofibrous hydroxyethyl cellulose/ poly (vinyl alcohol) scaffolds for skin tissue engineering
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/6020/ http://umpir.ump.edu.my/id/eprint/6020/1/fsti-2014-Fathima_Shahitha-fabrication_of_nanofibrous.pdf http://umpir.ump.edu.my/id/eprint/6020/2/Nanosmat_presentation.pdf
first_indexed	2023-09-18T22:01:31Z
last_indexed	2023-09-18T22:01:31Z
_version_	1777414429989666816

Fabrication of Nanofibrous Hydroxyethyl Cellulose/ poly (vinyl alcohol) Scaffolds for Skin Tissue Engineering

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