Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity

Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity

Omega-3 polyunsaturated fatty acid PUFA) plays a vital role in a number of human health aspects substantially for the regulation of biological function,prevention and treatment of human diseases. Subsequently,humans cannot synthesize the omega-3 PUFA,eicosapentaenoic acid(EPA)and docosahexaenoic aci...

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Main Author: Nor Farahiyah, Ghazali
Format: Undergraduates Project Papers
Language:English
Published: 2012
Subjects:
QP Physiology
Online Access:http://umpir.ump.edu.my/id/eprint/5909/
http://umpir.ump.edu.my/id/eprint/5909/
http://umpir.ump.edu.my/id/eprint/5909/1/CD7136.pdf
id ump-5909
recordtype eprints
spelling ump-59092015-03-03T09:27:09Z http://umpir.ump.edu.my/id/eprint/5909/ Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity Nor Farahiyah, Ghazali QP Physiology Omega-3 polyunsaturated fatty acid PUFA) plays a vital role in a number of human health aspects substantially for the regulation of biological function,prevention and treatment of human diseases. Subsequently,humans cannot synthesize the omega-3 PUFA,eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA),thus,intake comes predominantly from dietary. Therefore,the microalgae was discovered as an alternative source of fatty acids compared to fish oil since it offers better purification essential and has no risk of chemical contamination.Moreover,it also acts as a carbon dioxide fixer by photosynthesis process in reducing the atmospheric carbon dioxide.There are major factors give affect for microalgae growth which is carbon source,irradiance level, temperature,nutrient availability and salinity.This study is carried out to investigate carbon dioxide effect in the specific range of 2%, 5%, 10% and 15% at flow rate of 0.2 L/min.Meanwhile,the irradiance levels are examined by using 31 µmol photons m-2 s-1, 82 µmol photons m-2 s-1 ,125 µmol photons m-2 s-1 and 156 µmol photons m-2 s-1 by applying one factor at time (OFAT) in maximizing the production of omega-3 fatty acids.The microalgae of Nannochloropsis sp.are cultured in f/2 medium with 300 ml working volume for 7 days of cultivation in an experimental system of photobioreactor.The omega-3 fatty acid compositions of Nannochloropsis sp.under different culture conditions are analyzed by lipid extraction and gas chromatography analysis in order to acquire the maximum amount of yield production.The results showed that the highest eicosapentaenoic acid productivity by Nannochloropsis sp.had been obtained at 2% of carbon dioxide aeration and 156 µmol photons m-2 s-1 which are 31.3121 mg/ml and 35.1339 mg/ml, respectively. The fatty acid accumulation of Nannochloropsis sp.could be prevailed to produce a high yield under their optimal conditions which are in maximal efficiency of carbon dioxide and high of light intensity. 2012-07 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5909/1/CD7136.pdf Nor Farahiyah, Ghazali (2012) Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:75817&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QP Physiology
spellingShingle QP Physiology
Nor Farahiyah, Ghazali
Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity
description Omega-3 polyunsaturated fatty acid PUFA) plays a vital role in a number of human health aspects substantially for the regulation of biological function,prevention and treatment of human diseases. Subsequently,humans cannot synthesize the omega-3 PUFA,eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA),thus,intake comes predominantly from dietary. Therefore,the microalgae was discovered as an alternative source of fatty acids compared to fish oil since it offers better purification essential and has no risk of chemical contamination.Moreover,it also acts as a carbon dioxide fixer by photosynthesis process in reducing the atmospheric carbon dioxide.There are major factors give affect for microalgae growth which is carbon source,irradiance level, temperature,nutrient availability and salinity.This study is carried out to investigate carbon dioxide effect in the specific range of 2%, 5%, 10% and 15% at flow rate of 0.2 L/min.Meanwhile,the irradiance levels are examined by using 31 µmol photons m-2 s-1, 82 µmol photons m-2 s-1 ,125 µmol photons m-2 s-1 and 156 µmol photons m-2 s-1 by applying one factor at time (OFAT) in maximizing the production of omega-3 fatty acids.The microalgae of Nannochloropsis sp.are cultured in f/2 medium with 300 ml working volume for 7 days of cultivation in an experimental system of photobioreactor.The omega-3 fatty acid compositions of Nannochloropsis sp.under different culture conditions are analyzed by lipid extraction and gas chromatography analysis in order to acquire the maximum amount of yield production.The results showed that the highest eicosapentaenoic acid productivity by Nannochloropsis sp.had been obtained at 2% of carbon dioxide aeration and 156 µmol photons m-2 s-1 which are 31.3121 mg/ml and 35.1339 mg/ml, respectively. The fatty acid accumulation of Nannochloropsis sp.could be prevailed to produce a high yield under their optimal conditions which are in maximal efficiency of carbon dioxide and high of light intensity.
format Undergraduates Project Papers
author Nor Farahiyah, Ghazali
author_facet Nor Farahiyah, Ghazali
author_sort Nor Farahiyah, Ghazali
title Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity
title_short Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity
title_full Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity
title_fullStr Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity
title_full_unstemmed Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity
title_sort production of omega-3 fatty acid from nannochloropsis sp. on the effects of carbon dioxide and light intensity
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/5909/
http://umpir.ump.edu.my/id/eprint/5909/
http://umpir.ump.edu.my/id/eprint/5909/1/CD7136.pdf
first_indexed 2023-09-18T22:01:20Z
last_indexed 2023-09-18T22:01:20Z
_version_ 1777414418156486656

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