Production and characterization of protein extract from lempoyang ginger

Antimicrobial protein has received tremendous attention especially by the pharmaceutical industry,which sources mainly extracted from nature such as herbs and plants.These sources are known for their nontoxic property, biodegradable,as well as easily available in the market. Among the numerous natur...

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Main Author:	Mellisa, Abdullah
Format:	Undergraduates Project Papers
Language:	English
Published:	2012
Subjects:	QK Botany
Online Access:	http://umpir.ump.edu.my/id/eprint/5908/ http://umpir.ump.edu.my/id/eprint/5908/ http://umpir.ump.edu.my/id/eprint/5908/1/CD7134.pdf

id	ump-5908
recordtype	eprints
spelling	ump-59082015-03-03T09:27:09Z http://umpir.ump.edu.my/id/eprint/5908/ Production and characterization of protein extract from lempoyang ginger Mellisa, Abdullah QK Botany Antimicrobial protein has received tremendous attention especially by the pharmaceutical industry,which sources mainly extracted from nature such as herbs and plants.These sources are known for their nontoxic property, biodegradable,as well as easily available in the market. Among the numerous natural resources,the Lempoyang ginger which scientifically known as Zingiber zerumbet L.Smith had long known for its application as antifungal,anti-inflammatory,anti-ulceration and antioxidant.It is also known for treating diarrhea,coughs,asthma and some other skin diseases.Lempoyang ginger is selected for this research as very few information was published on protein of this Zingiberaceae family of which among all,zerumbone was widely extracted as the major component.This research aimed to produce the protein extract from Lempoyang using the expanded bed adsorption chromatography(EBAC)and then characterize it using the sodium dodecylsulfate polyacrylamide gel electrophoresis(SDS-PAGE).The parameter that was varied for this research is the height of the bed settlement at 5cm,6cm,9cm and 11cm,with elution made in two-steps using 45% ethanol and 90% ethanol buffer solutions. Amberlite XAD7HP is used as adsorbents to bind the large complex protein molecules from Lempoyang feedstock with constant pump speed at 13rpm for each cycle.The eluted protein fractions are then subjected to Lowry‟s protein concentration determination technique before characterized for molecular weight determination using the SDS-PAGE tools. The dynamic binding capacity is determined from the 50% breakthrough of the initial feedstock solution,and the recovery ratio is determined for total protein obtained. From the results,protein concentration is higher when eluted with 45% ethanol buffer solution as protein were loosely bounded to the adsorbents while the first elution using 90% ethanol buffer solution showed a lower protein concentration.The washed protein and eluted protein fractions from EBAC which were tested on SDS-PAGE showed a protein band of eluted fraction protein band with molecular weight of 21.12 kDa. 2012-02 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5908/1/CD7134.pdf Mellisa, Abdullah (2012) Production and characterization of protein extract from lempoyang ginger. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:75808&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QK Botany
spellingShingle	QK Botany Mellisa, Abdullah Production and characterization of protein extract from lempoyang ginger
description	Antimicrobial protein has received tremendous attention especially by the pharmaceutical industry,which sources mainly extracted from nature such as herbs and plants.These sources are known for their nontoxic property, biodegradable,as well as easily available in the market. Among the numerous natural resources,the Lempoyang ginger which scientifically known as Zingiber zerumbet L.Smith had long known for its application as antifungal,anti-inflammatory,anti-ulceration and antioxidant.It is also known for treating diarrhea,coughs,asthma and some other skin diseases.Lempoyang ginger is selected for this research as very few information was published on protein of this Zingiberaceae family of which among all,zerumbone was widely extracted as the major component.This research aimed to produce the protein extract from Lempoyang using the expanded bed adsorption chromatography(EBAC)and then characterize it using the sodium dodecylsulfate polyacrylamide gel electrophoresis(SDS-PAGE).The parameter that was varied for this research is the height of the bed settlement at 5cm,6cm,9cm and 11cm,with elution made in two-steps using 45% ethanol and 90% ethanol buffer solutions. Amberlite XAD7HP is used as adsorbents to bind the large complex protein molecules from Lempoyang feedstock with constant pump speed at 13rpm for each cycle.The eluted protein fractions are then subjected to Lowry‟s protein concentration determination technique before characterized for molecular weight determination using the SDS-PAGE tools. The dynamic binding capacity is determined from the 50% breakthrough of the initial feedstock solution,and the recovery ratio is determined for total protein obtained. From the results,protein concentration is higher when eluted with 45% ethanol buffer solution as protein were loosely bounded to the adsorbents while the first elution using 90% ethanol buffer solution showed a lower protein concentration.The washed protein and eluted protein fractions from EBAC which were tested on SDS-PAGE showed a protein band of eluted fraction protein band with molecular weight of 21.12 kDa.
format	Undergraduates Project Papers
author	Mellisa, Abdullah
author_facet	Mellisa, Abdullah
author_sort	Mellisa, Abdullah
title	Production and characterization of protein extract from lempoyang ginger
title_short	Production and characterization of protein extract from lempoyang ginger
title_full	Production and characterization of protein extract from lempoyang ginger
title_fullStr	Production and characterization of protein extract from lempoyang ginger
title_full_unstemmed	Production and characterization of protein extract from lempoyang ginger
title_sort	production and characterization of protein extract from lempoyang ginger
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/5908/ http://umpir.ump.edu.my/id/eprint/5908/ http://umpir.ump.edu.my/id/eprint/5908/1/CD7134.pdf
first_indexed	2023-09-18T22:01:20Z
last_indexed	2023-09-18T22:01:20Z
_version_	1777414418009686016

Production and characterization of protein extract from lempoyang ginger

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