Screening of lignin degrader from soil

Lignin creates the main problem in waste from the pulp and paper industry. The pulp and paper industry is always interested in finding new, cheaper and more effective ways of get lignin out so that they can get to the cellulose fibres. Lignin degrading bacteria and enzyme is characterized. Important...

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Main Author: Yeoh, Teik Loon
Format: Undergraduates Project Papers
Language:English
Published: 2006
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/589/
http://umpir.ump.edu.my/id/eprint/589/
http://umpir.ump.edu.my/id/eprint/589/1/Yeoh%2C_Teik_Loon.pdf
id ump-589
recordtype eprints
spelling ump-5892015-03-03T06:04:18Z http://umpir.ump.edu.my/id/eprint/589/ Screening of lignin degrader from soil Yeoh, Teik Loon TP Chemical technology Lignin creates the main problem in waste from the pulp and paper industry. The pulp and paper industry is always interested in finding new, cheaper and more effective ways of get lignin out so that they can get to the cellulose fibres. Lignin degrading bacteria and enzyme is characterized. Important aspects of the production of the enzyme by lignin degrading bacteria studied include the characterization of enzyme and improvement of yield. This process includes a two phases: screening process and the characterization process. Screening process was done using selective agar containing alkaline lignin. The microbe was characterized using gram staining, spore staining and acid-fast staining. The lignin degrading microbe found was tested; the result was a gramnegative bacteria, has no endospore and is not acid fast. The optimum pH found was 6.0 while the optimum temperature examined was 40°C and gave the maximum enzyme activity of 2.00E-05μmol/ml/min. The effect of carbon source and nitrogen source on the enzyme production was also studied. Carbon sources that were compared were carboxy methyl cellulose, glucose and xylose. It was proven that xylose is the best carbon source among the three. While nitrogen sources compared were yeast extract, peptone and urea. The best nitrogen source among the three was yeast extract and peptone. 2006-11 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/589/1/Yeoh%2C_Teik_Loon.pdf Yeoh, Teik Loon (2006) Screening of lignin degrader from soil. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:19090&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Yeoh, Teik Loon
Screening of lignin degrader from soil
description Lignin creates the main problem in waste from the pulp and paper industry. The pulp and paper industry is always interested in finding new, cheaper and more effective ways of get lignin out so that they can get to the cellulose fibres. Lignin degrading bacteria and enzyme is characterized. Important aspects of the production of the enzyme by lignin degrading bacteria studied include the characterization of enzyme and improvement of yield. This process includes a two phases: screening process and the characterization process. Screening process was done using selective agar containing alkaline lignin. The microbe was characterized using gram staining, spore staining and acid-fast staining. The lignin degrading microbe found was tested; the result was a gramnegative bacteria, has no endospore and is not acid fast. The optimum pH found was 6.0 while the optimum temperature examined was 40°C and gave the maximum enzyme activity of 2.00E-05μmol/ml/min. The effect of carbon source and nitrogen source on the enzyme production was also studied. Carbon sources that were compared were carboxy methyl cellulose, glucose and xylose. It was proven that xylose is the best carbon source among the three. While nitrogen sources compared were yeast extract, peptone and urea. The best nitrogen source among the three was yeast extract and peptone.
format Undergraduates Project Papers
author Yeoh, Teik Loon
author_facet Yeoh, Teik Loon
author_sort Yeoh, Teik Loon
title Screening of lignin degrader from soil
title_short Screening of lignin degrader from soil
title_full Screening of lignin degrader from soil
title_fullStr Screening of lignin degrader from soil
title_full_unstemmed Screening of lignin degrader from soil
title_sort screening of lignin degrader from soil
publishDate 2006
url http://umpir.ump.edu.my/id/eprint/589/
http://umpir.ump.edu.my/id/eprint/589/
http://umpir.ump.edu.my/id/eprint/589/1/Yeoh%2C_Teik_Loon.pdf
first_indexed 2023-09-18T21:52:58Z
last_indexed 2023-09-18T21:52:58Z
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