Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration

ABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. Th...

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Main Author:	Norhalmy, Mohd Nor
Format:	Undergraduates Project Papers
Language:	English
Published:	2006
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/559/ http://umpir.ump.edu.my/id/eprint/559/ http://umpir.ump.edu.my/id/eprint/559/1/Norhalmy_B._Mohd_Nor.pdf

id	ump-559
recordtype	eprints
spelling	ump-5592015-03-03T06:04:08Z http://umpir.ump.edu.my/id/eprint/559/ Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration Norhalmy, Mohd Nor TP Chemical technology ABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. The study is focus on the effect of substrate concentration which is 1 g/L, 3g/L and 5 g/L. All the enzymes were incubating in temperature 37 oC and pH 5.0 for 30 minutes. The reaction was quenched by cooling on ice and the amount of reducing sugar was measured by dinitrosalicyclic (DNS) acid procedure with slight modification (Miller, 1965). The mixture was then incubated at 100 oC for 5 minutes. Subsequently, the reaction was stopped by cooling on ice. Distilled water was added into the final volume of 16 ml and the color intensity was measured at wavelength of 575 nm. The concentration of SCB affected the xylanase and CMCase activity. Xylanase and carboxymethylcellulase activity was higher at lower concentration hen using 3 g of SCB. 2006-11 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/559/1/Norhalmy_B._Mohd_Nor.pdf Norhalmy, Mohd Nor (2006) Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:18577&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Norhalmy, Mohd Nor Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
description	ABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. The study is focus on the effect of substrate concentration which is 1 g/L, 3g/L and 5 g/L. All the enzymes were incubating in temperature 37 oC and pH 5.0 for 30 minutes. The reaction was quenched by cooling on ice and the amount of reducing sugar was measured by dinitrosalicyclic (DNS) acid procedure with slight modification (Miller, 1965). The mixture was then incubated at 100 oC for 5 minutes. Subsequently, the reaction was stopped by cooling on ice. Distilled water was added into the final volume of 16 ml and the color intensity was measured at wavelength of 575 nm. The concentration of SCB affected the xylanase and CMCase activity. Xylanase and carboxymethylcellulase activity was higher at lower concentration hen using 3 g of SCB.
format	Undergraduates Project Papers
author	Norhalmy, Mohd Nor
author_facet	Norhalmy, Mohd Nor
author_sort	Norhalmy, Mohd Nor
title	Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
title_short	Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
title_full	Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
title_fullStr	Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
title_full_unstemmed	Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
title_sort	production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
publishDate	2006
url	http://umpir.ump.edu.my/id/eprint/559/ http://umpir.ump.edu.my/id/eprint/559/ http://umpir.ump.edu.my/id/eprint/559/1/Norhalmy_B._Mohd_Nor.pdf
first_indexed	2023-09-18T21:52:54Z
last_indexed	2023-09-18T21:52:54Z
_version_	1777413887805620224

Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration

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