Preliminary study on parameters of lysozyme purification using ion exchange chromatography

Lysozyme is high demand bioproduct in market nowadays produced by protein purification process. Chromatography is a multicomponent separation technique which primarily used in the biotechnology sector especially in protein purification. The physical method of chromatography separation in which the c...

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Main Author:	Wan Norlinda Roshana, Mohd Nawi
Format:	Undergraduates Project Papers
Language:	English
Published:	2006
Subjects:	QP Physiology
Online Access:	http://umpir.ump.edu.my/id/eprint/539/ http://umpir.ump.edu.my/id/eprint/539/ http://umpir.ump.edu.my/id/eprint/539/1/Wan_Norlinda_Roshana_Binti_Mohd_Nawi.pdf

id	ump-539
recordtype	eprints
spelling	ump-5392018-08-09T06:54:48Z http://umpir.ump.edu.my/id/eprint/539/ Preliminary study on parameters of lysozyme purification using ion exchange chromatography Wan Norlinda Roshana, Mohd Nawi QP Physiology Lysozyme is high demand bioproduct in market nowadays produced by protein purification process. Chromatography is a multicomponent separation technique which primarily used in the biotechnology sector especially in protein purification. The physical method of chromatography separation in which the components to be separated are distributed in stationary phase and mobile phase. One of the chromatography techniques that widely used is ion exchange chromatography (IEC) which based on the electrostatic interaction. The basic theory of the IEC separation process is depending on the isoelectric of the protein (pI). The pI of the protein is influence to determine the media of IEC whether anion or cation exchanger been used. In the experimental work, the automated liquid chromatography system from Amersham-Pharmacia Biosciences is designed for method development and research application whereas calibrated and controlled by a computer using UNICORN software. Preliminary study on the parameters is based on pH and salt concentration which to achieve the high purity of lysozyme. Some purification experiments were done to find the optimal operation condition to purify lysozyme from protein mixture by changing buffer pH and salt concentration in elution buffer to elute the interest protein. Based on the result and discussion; by manipulating pH value, the highest height detected at pH 8.5 which is 2.680. Using calibration curve, concentration of lysozyme is 0.0315 mg/ml. When NaCl concentration was manipulated, the concentration was optimizing at 0.3 M with is the result is 0.0284 mg/ml. As the conclusion, the optimum condition in purification step at pH buffer 8.5 and elution buffer at 0.3 M which is almost to the expected result refer to previous study. 2006-11 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/539/1/Wan_Norlinda_Roshana_Binti_Mohd_Nawi.pdf Wan Norlinda Roshana, Mohd Nawi (2006) Preliminary study on parameters of lysozyme purification using ion exchange chromatography. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:18418&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QP Physiology
spellingShingle	QP Physiology Wan Norlinda Roshana, Mohd Nawi Preliminary study on parameters of lysozyme purification using ion exchange chromatography
description	Lysozyme is high demand bioproduct in market nowadays produced by protein purification process. Chromatography is a multicomponent separation technique which primarily used in the biotechnology sector especially in protein purification. The physical method of chromatography separation in which the components to be separated are distributed in stationary phase and mobile phase. One of the chromatography techniques that widely used is ion exchange chromatography (IEC) which based on the electrostatic interaction. The basic theory of the IEC separation process is depending on the isoelectric of the protein (pI). The pI of the protein is influence to determine the media of IEC whether anion or cation exchanger been used. In the experimental work, the automated liquid chromatography system from Amersham-Pharmacia Biosciences is designed for method development and research application whereas calibrated and controlled by a computer using UNICORN software. Preliminary study on the parameters is based on pH and salt concentration which to achieve the high purity of lysozyme. Some purification experiments were done to find the optimal operation condition to purify lysozyme from protein mixture by changing buffer pH and salt concentration in elution buffer to elute the interest protein. Based on the result and discussion; by manipulating pH value, the highest height detected at pH 8.5 which is 2.680. Using calibration curve, concentration of lysozyme is 0.0315 mg/ml. When NaCl concentration was manipulated, the concentration was optimizing at 0.3 M with is the result is 0.0284 mg/ml. As the conclusion, the optimum condition in purification step at pH buffer 8.5 and elution buffer at 0.3 M which is almost to the expected result refer to previous study.
format	Undergraduates Project Papers
author	Wan Norlinda Roshana, Mohd Nawi
author_facet	Wan Norlinda Roshana, Mohd Nawi
author_sort	Wan Norlinda Roshana, Mohd Nawi
title	Preliminary study on parameters of lysozyme purification using ion exchange chromatography
title_short	Preliminary study on parameters of lysozyme purification using ion exchange chromatography
title_full	Preliminary study on parameters of lysozyme purification using ion exchange chromatography
title_fullStr	Preliminary study on parameters of lysozyme purification using ion exchange chromatography
title_full_unstemmed	Preliminary study on parameters of lysozyme purification using ion exchange chromatography
title_sort	preliminary study on parameters of lysozyme purification using ion exchange chromatography
publishDate	2006
url	http://umpir.ump.edu.my/id/eprint/539/ http://umpir.ump.edu.my/id/eprint/539/ http://umpir.ump.edu.my/id/eprint/539/1/Wan_Norlinda_Roshana_Binti_Mohd_Nawi.pdf
first_indexed	2023-09-18T21:52:51Z
last_indexed	2023-09-18T21:52:51Z
_version_	1777413885210394624

Preliminary study on parameters of lysozyme purification using ion exchange chromatography

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