Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature

Natural by product of agricultural waste can be turned to products of commercial interests such as ethanol, glucose and single cell protein. Much effort from scientists and researches all around the world has been put to extend the full use of agricultural waste. Reports of cellulase enzyme producti...

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Main Author:	Mohd. Nazip, Kassebullah
Format:	Undergraduates Project Papers
Language:	English
Published:	2006
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/538/ http://umpir.ump.edu.my/id/eprint/538/ http://umpir.ump.edu.my/id/eprint/538/1/Mohd._Nazip_Kassebullah.pdf

id	ump-538
recordtype	eprints
spelling	ump-5382015-03-03T06:04:00Z http://umpir.ump.edu.my/id/eprint/538/ Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature Mohd. Nazip, Kassebullah TP Chemical technology Natural by product of agricultural waste can be turned to products of commercial interests such as ethanol, glucose and single cell protein. Much effort from scientists and researches all around the world has been put to extend the full use of agricultural waste. Reports of cellulase enzyme production from the bioconversion of lignocellulosics materials has much been made. However there is still much space to find the most suitable condition. In this research, sugar cane bagasse which is used as substrate is subjected to physical treatment using milling and grinding. After that, sugar cane bagasse is use as substrate in the production of cellulase enzyme using Aspergillus terreus SUK-1 fungus. Cellulase enzyme production was studied using submerged fermentation under shaking. The production was run in a lab scale using Erlenmeyer flask only. After that the enzyme was assayed at different temperature. The purpose of this research was to investigate the effect of substrate concentration on yield of cellulase and the optimum temperature where the yield of enzyme was the highest. Analysis is based on the activity of carboxymethylcellulase and xylanase enzymes. Result shows that the peak of xylanase activities was 0.011322 U/ml on day 5 using 5g SCB as substrate and fermentation temperature of 50°C while carboxymethylcellulase peak was 0.007985049 U/ml of activity using 3g SCB as substrate and fermentation temperature of 40°C. Xylanase activity was high when assayed at 50°C while carboxymethylcellulase activity was high when assayed at 40°C. 2006-11 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/538/1/Mohd._Nazip_Kassebullah.pdf Mohd. Nazip, Kassebullah (2006) Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:18739&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mohd. Nazip, Kassebullah Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature
description	Natural by product of agricultural waste can be turned to products of commercial interests such as ethanol, glucose and single cell protein. Much effort from scientists and researches all around the world has been put to extend the full use of agricultural waste. Reports of cellulase enzyme production from the bioconversion of lignocellulosics materials has much been made. However there is still much space to find the most suitable condition. In this research, sugar cane bagasse which is used as substrate is subjected to physical treatment using milling and grinding. After that, sugar cane bagasse is use as substrate in the production of cellulase enzyme using Aspergillus terreus SUK-1 fungus. Cellulase enzyme production was studied using submerged fermentation under shaking. The production was run in a lab scale using Erlenmeyer flask only. After that the enzyme was assayed at different temperature. The purpose of this research was to investigate the effect of substrate concentration on yield of cellulase and the optimum temperature where the yield of enzyme was the highest. Analysis is based on the activity of carboxymethylcellulase and xylanase enzymes. Result shows that the peak of xylanase activities was 0.011322 U/ml on day 5 using 5g SCB as substrate and fermentation temperature of 50°C while carboxymethylcellulase peak was 0.007985049 U/ml of activity using 3g SCB as substrate and fermentation temperature of 40°C. Xylanase activity was high when assayed at 50°C while carboxymethylcellulase activity was high when assayed at 40°C.
format	Undergraduates Project Papers
author	Mohd. Nazip, Kassebullah
author_facet	Mohd. Nazip, Kassebullah
author_sort	Mohd. Nazip, Kassebullah
title	Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature
title_short	Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature
title_full	Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature
title_fullStr	Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature
title_full_unstemmed	Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature
title_sort	production of cellulase enzyme from aspergillus terreues suk-1 using sugar cane waste : the effect of substrate concentration and assay temperature
publishDate	2006
url	http://umpir.ump.edu.my/id/eprint/538/ http://umpir.ump.edu.my/id/eprint/538/ http://umpir.ump.edu.my/id/eprint/538/1/Mohd._Nazip_Kassebullah.pdf
first_indexed	2023-09-18T21:52:51Z
last_indexed	2023-09-18T21:52:51Z
_version_	1777413885074079744

Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature

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