Effect of Substrate and Enzyme Concentration on Cyclodextrin Production in a Hollow Fibre Membrane Reactor System

The batch and continuous production of cyclodextrins (CDs) was assessed by employing an enzymatic membrane reactor (EMR) system. The effects of tapioca starch substrate and cyclodextrin glycosyltransferase (CGTase) concentrations on the yield of CDs were studied. A similar effect on the behaviour of...

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Main Authors:	Mimi Sakinah, A. M., Ismail, A. F., Rosli, Md. Illias, Zularisam, A. W., Osman, Hassan, Matsuura, T.
Format:	Article
Language:	English
Published:	Elsevier 2014
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/5333/ http://umpir.ump.edu.my/id/eprint/5333/ http://umpir.ump.edu.my/id/eprint/5333/ http://umpir.ump.edu.my/id/eprint/5333/1/fkksa-2014-mimi-effect_of_substrate_abs_only.pdf

id	ump-5333
recordtype	eprints
spelling	ump-53332018-01-16T07:59:49Z http://umpir.ump.edu.my/id/eprint/5333/ Effect of Substrate and Enzyme Concentration on Cyclodextrin Production in a Hollow Fibre Membrane Reactor System Mimi Sakinah, A. M. Ismail, A. F. Rosli, Md. Illias Zularisam, A. W. Osman, Hassan Matsuura, T. TP Chemical technology The batch and continuous production of cyclodextrins (CDs) was assessed by employing an enzymatic membrane reactor (EMR) system. The effects of tapioca starch substrate and cyclodextrin glycosyltransferase (CGTase) concentrations on the yield of CDs were studied. A similar effect on the behaviour of the ultrafiltration membrane in the EMR system (integration system) was also evaluated. The results for the batch process showed that incremental doses of CGTase caused gradual increments in CD yield; however, further addition of CGTase (above 1.0%) showed a 16% reduction in the total CD production. Further incremental in the tapioca starch concentration increased CD concentration (23 g/L). However, addition above 8% w/v resulted in an insignificant yield of CDs. In the case of integration system, tapioca starch feeding rate that is higher than 4.41 g/h caused adverse effects (lower CD yield and membrane flux). In particular at higher tapioca starch feeding rate (5.0 g/h), the hydraulic resistance would reach as high as 1.31 × 1013 m−1. Presumably this phenomenon was due to the unreacted substrates that were adsorbing onto the membrane surface and pores that subsequently led to greater fouling conditions and a severe flux decline. In addition, the weak adsorption (ra1) has been found to be the major fouling mechanism attributed to starch and its by products. Therefore, hydraulic cleaning is highly suggested as the procedure to be used for this EMR system. Elsevier 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5333/1/fkksa-2014-mimi-effect_of_substrate_abs_only.pdf Mimi Sakinah, A. M. and Ismail, A. F. and Rosli, Md. Illias and Zularisam, A. W. and Osman, Hassan and Matsuura, T. (2014) Effect of Substrate and Enzyme Concentration on Cyclodextrin Production in a Hollow Fibre Membrane Reactor System. Separation and Purification Technology, 124. pp. 61-67. ISSN 1383-5866 http://dx.doi.org/10.1016/j.seppur.2014.01.005 DOI: 10.1016/j.seppur.2014.01.005
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mimi Sakinah, A. M. Ismail, A. F. Rosli, Md. Illias Zularisam, A. W. Osman, Hassan Matsuura, T. Effect of Substrate and Enzyme Concentration on Cyclodextrin Production in a Hollow Fibre Membrane Reactor System
description	The batch and continuous production of cyclodextrins (CDs) was assessed by employing an enzymatic membrane reactor (EMR) system. The effects of tapioca starch substrate and cyclodextrin glycosyltransferase (CGTase) concentrations on the yield of CDs were studied. A similar effect on the behaviour of the ultrafiltration membrane in the EMR system (integration system) was also evaluated. The results for the batch process showed that incremental doses of CGTase caused gradual increments in CD yield; however, further addition of CGTase (above 1.0%) showed a 16% reduction in the total CD production. Further incremental in the tapioca starch concentration increased CD concentration (23 g/L). However, addition above 8% w/v resulted in an insignificant yield of CDs. In the case of integration system, tapioca starch feeding rate that is higher than 4.41 g/h caused adverse effects (lower CD yield and membrane flux). In particular at higher tapioca starch feeding rate (5.0 g/h), the hydraulic resistance would reach as high as 1.31 × 1013 m−1. Presumably this phenomenon was due to the unreacted substrates that were adsorbing onto the membrane surface and pores that subsequently led to greater fouling conditions and a severe flux decline. In addition, the weak adsorption (ra1) has been found to be the major fouling mechanism attributed to starch and its by products. Therefore, hydraulic cleaning is highly suggested as the procedure to be used for this EMR system.
format	Article
author	Mimi Sakinah, A. M. Ismail, A. F. Rosli, Md. Illias Zularisam, A. W. Osman, Hassan Matsuura, T.
author_facet	Mimi Sakinah, A. M. Ismail, A. F. Rosli, Md. Illias Zularisam, A. W. Osman, Hassan Matsuura, T.
author_sort	Mimi Sakinah, A. M.
title	Effect of Substrate and Enzyme Concentration on Cyclodextrin Production in a Hollow Fibre Membrane Reactor System
title_short	Effect of Substrate and Enzyme Concentration on Cyclodextrin Production in a Hollow Fibre Membrane Reactor System
title_full	Effect of Substrate and Enzyme Concentration on Cyclodextrin Production in a Hollow Fibre Membrane Reactor System
title_fullStr	Effect of Substrate and Enzyme Concentration on Cyclodextrin Production in a Hollow Fibre Membrane Reactor System
title_full_unstemmed	Effect of Substrate and Enzyme Concentration on Cyclodextrin Production in a Hollow Fibre Membrane Reactor System
title_sort	effect of substrate and enzyme concentration on cyclodextrin production in a hollow fibre membrane reactor system
publisher	Elsevier
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/5333/ http://umpir.ump.edu.my/id/eprint/5333/ http://umpir.ump.edu.my/id/eprint/5333/ http://umpir.ump.edu.my/id/eprint/5333/1/fkksa-2014-mimi-effect_of_substrate_abs_only.pdf
first_indexed	2023-09-18T22:00:38Z
last_indexed	2023-09-18T22:00:38Z
_version_	1777414374394167296

Effect of Substrate and Enzyme Concentration on Cyclodextrin Production in a Hollow Fibre Membrane Reactor System

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