Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters

Superovulation has become a common assisted reproductive technology in the field of animal reproduction. In addition, zona-free hamster oocytes have been used in heterologous in vitro fertilization research to evaluate sperm function. A study was conducted to compare eight different superovulation p...

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Main Authors:	Jaya Vejayan, Palliah, Sarsaifi, Kazhal, Omar, Mohamed-Ariff, Rosnina, H. Yusoff, Abd-Wahid, Haron, Hani, Homayoun, Yimer, Nurhusein, Naing, Soe W., Abas Mazni, Othman
Format:	Article
Language:	English
Published:	Science Alert 2013
Subjects:	Q Science (General) QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/5240/ http://umpir.ump.edu.my/id/eprint/5240/ http://umpir.ump.edu.my/id/eprint/5240/ http://umpir.ump.edu.my/id/eprint/5240/1/journal.pdf

id	ump-5240
recordtype	eprints
spelling	ump-52402018-02-21T06:57:53Z http://umpir.ump.edu.my/id/eprint/5240/ Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters Jaya Vejayan, Palliah Sarsaifi, Kazhal Omar, Mohamed-Ariff Rosnina, H. Yusoff Abd-Wahid, Haron Hani, Homayoun Yimer, Nurhusein Naing, Soe W. Abas Mazni, Othman Q Science (General) QR Microbiology Superovulation has become a common assisted reproductive technology in the field of animal reproduction. In addition, zona-free hamster oocytes have been used in heterologous in vitro fertilization research to evaluate sperm function. A study was conducted to compare eight different superovulation protocols for golden hamsters using two concentrations of human Chorionic Gonadotropin (hCG) given at two time intervals post-pregnant mare’s serum gonadotrophin (PMSG) injection and two time intervals of oocyte harvesting. Fifty-six female golden hamsters were randomly and equally assigned into eight superovulation groups. Hamsters were superovulated initially with PMSG followed by human Chorionic Gonadotrophin (hCG). All the groups received 40 IU PMSG, either 40 or 45 IU hCG given at either 48-50 or 55-57 h post PMSG injection and the oocytes recovered at either 12-15 or 16-18 h after hCG injection. Higher number of recovered oocytes (51.57±0.83) and maturation rates (94.20%) (p<0.05) were detected in hamsters which received 45 IU hCG at 55-57 h after PMSG injection when the oocytes were recovered later at 16-18 h compared with hamsters in the other groups. Mean fertilization rate of hamsters given 45 IU hCG at 55-57 h post PMSG injection ranged from 77.89-78.84% and were significantly higher (p<0.05) than those that received hCG at 48-50 h post PMSG injection. In conclusion administration of 40 IU PMSG followed by 45 IU hCG injection at 55 and 57 h post PMSG injection followed by oocyte recovery after 16-18 h gave the highest response in oocyte recovery and maturation in golden hamsters. Science Alert 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5240/1/journal.pdf Jaya Vejayan, Palliah and Sarsaifi, Kazhal and Omar, Mohamed-Ariff and Rosnina, H. Yusoff and Abd-Wahid, Haron and Hani, Homayoun and Yimer, Nurhusein and Naing, Soe W. and Abas Mazni, Othman (2013) Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters. Asian Journal of Animal and Veterinary Advances, 8 (6). pp. 796-805. ISSN 1683-9919 (print); 1996-3289 (online) http://dx.doi.org/10.3923/ajava.2013.796.805 DOI: 10.3923/ajava.2013.796.805
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) QR Microbiology
spellingShingle	Q Science (General) QR Microbiology Jaya Vejayan, Palliah Sarsaifi, Kazhal Omar, Mohamed-Ariff Rosnina, H. Yusoff Abd-Wahid, Haron Hani, Homayoun Yimer, Nurhusein Naing, Soe W. Abas Mazni, Othman Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
description	Superovulation has become a common assisted reproductive technology in the field of animal reproduction. In addition, zona-free hamster oocytes have been used in heterologous in vitro fertilization research to evaluate sperm function. A study was conducted to compare eight different superovulation protocols for golden hamsters using two concentrations of human Chorionic Gonadotropin (hCG) given at two time intervals post-pregnant mare’s serum gonadotrophin (PMSG) injection and two time intervals of oocyte harvesting. Fifty-six female golden hamsters were randomly and equally assigned into eight superovulation groups. Hamsters were superovulated initially with PMSG followed by human Chorionic Gonadotrophin (hCG). All the groups received 40 IU PMSG, either 40 or 45 IU hCG given at either 48-50 or 55-57 h post PMSG injection and the oocytes recovered at either 12-15 or 16-18 h after hCG injection. Higher number of recovered oocytes (51.57±0.83) and maturation rates (94.20%) (p<0.05) were detected in hamsters which received 45 IU hCG at 55-57 h after PMSG injection when the oocytes were recovered later at 16-18 h compared with hamsters in the other groups. Mean fertilization rate of hamsters given 45 IU hCG at 55-57 h post PMSG injection ranged from 77.89-78.84% and were significantly higher (p<0.05) than those that received hCG at 48-50 h post PMSG injection. In conclusion administration of 40 IU PMSG followed by 45 IU hCG injection at 55 and 57 h post PMSG injection followed by oocyte recovery after 16-18 h gave the highest response in oocyte recovery and maturation in golden hamsters.
format	Article
author	Jaya Vejayan, Palliah Sarsaifi, Kazhal Omar, Mohamed-Ariff Rosnina, H. Yusoff Abd-Wahid, Haron Hani, Homayoun Yimer, Nurhusein Naing, Soe W. Abas Mazni, Othman
author_facet	Jaya Vejayan, Palliah Sarsaifi, Kazhal Omar, Mohamed-Ariff Rosnina, H. Yusoff Abd-Wahid, Haron Hani, Homayoun Yimer, Nurhusein Naing, Soe W. Abas Mazni, Othman
author_sort	Jaya Vejayan, Palliah
title	Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title_short	Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title_full	Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title_fullStr	Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title_full_unstemmed	Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title_sort	development of superovulation program and heterologous in vitro fertilization test assessment in hamsters
publisher	Science Alert
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/5240/ http://umpir.ump.edu.my/id/eprint/5240/ http://umpir.ump.edu.my/id/eprint/5240/ http://umpir.ump.edu.my/id/eprint/5240/1/journal.pdf
first_indexed	2023-09-18T22:00:30Z
last_indexed	2023-09-18T22:00:30Z
_version_	1777414365788504064

Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters

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