Encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning

A drug delivery system is designed to provide a therapeutic agent in the needed amount,at the right time and to the proper location in the body in a manner that optimizes the efficacy,increases compliance and minimizes side effects.In order to study the encapsulation of therapeutic protein within po...

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Main Author:	Mohamad Muhaimin, Zalani
Format:	Undergraduates Project Papers
Language:	English
Published:	2012
Subjects:	TA Engineering (General). Civil engineering (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/5087/ http://umpir.ump.edu.my/id/eprint/5087/ http://umpir.ump.edu.my/id/eprint/5087/1/CD6489.pdf

id	ump-5087
recordtype	eprints
spelling	ump-50872015-03-03T09:23:02Z http://umpir.ump.edu.my/id/eprint/5087/ Encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning Mohamad Muhaimin, Zalani TA Engineering (General). Civil engineering (General) A drug delivery system is designed to provide a therapeutic agent in the needed amount,at the right time and to the proper location in the body in a manner that optimizes the efficacy,increases compliance and minimizes side effects.In order to study the encapsulation of therapeutic protein within polymeric nanofiber for controlled release using co-axial electrospinning method,a number of main processing parameters were taken into considerations which are formulation of drug loading,polymer and protein concentration and solution flow rate.Polymeric drug delivery device was developed via electrospinning technique using biodegradable Polymer A.Co-axial electrospinning configuration was used to encapsulate various mixtures of Drug 0066,Drug 0360 and also Polymer B as support into the electrospun nanofibers.Using the configuration,two separate solutions flowed through two different capillaries and electrospun through co-axial nozzle configuration setup.The electrified jet will undergo stretching, leading to formation of long and thin thread. When the liquid jet is continuously elongated, the solvent will evaporate.The grounded collector will attract the charged fiber.The morphology of the electrospun nanofibers were analyzed using Field Emission Scanning Electron Microscopy (FE-SEM) and Transmission Electron Microscopy(TEM).The hydrophilicity of electrospun nanofibers were determined using Surface Contact Angle machine.Fourier Transform Infrared Spectrometry(FT-IR)was used to detect the organic group of the electrospun nanofibers.In vitro release studies were conducted to evaluate sustained release potential of the core-sheath structure composite nanofiber.The results showed that the TEM images clearly proved the core/shell structure of nanofibers for the encapsulation of Drug 0066/Drug 0350 within Polymer A.SEM also showed there was an arch appeared within the nanofiber.The present study would provide a basis for further design and optimization of processing conditions to control the nanostructure of core-sheath composite nanofibers and ultimately achieve desired release kinetics of bioactive proteins(e.g., growth factors)for practical tissue engineering applications. 2012-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5087/1/CD6489.pdf Mohamad Muhaimin, Zalani (2012) Encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning. Faculty of Chemical & Natural Resource Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:67880&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TA Engineering (General). Civil engineering (General)
spellingShingle	TA Engineering (General). Civil engineering (General) Mohamad Muhaimin, Zalani Encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning
description	A drug delivery system is designed to provide a therapeutic agent in the needed amount,at the right time and to the proper location in the body in a manner that optimizes the efficacy,increases compliance and minimizes side effects.In order to study the encapsulation of therapeutic protein within polymeric nanofiber for controlled release using co-axial electrospinning method,a number of main processing parameters were taken into considerations which are formulation of drug loading,polymer and protein concentration and solution flow rate.Polymeric drug delivery device was developed via electrospinning technique using biodegradable Polymer A.Co-axial electrospinning configuration was used to encapsulate various mixtures of Drug 0066,Drug 0360 and also Polymer B as support into the electrospun nanofibers.Using the configuration,two separate solutions flowed through two different capillaries and electrospun through co-axial nozzle configuration setup.The electrified jet will undergo stretching, leading to formation of long and thin thread. When the liquid jet is continuously elongated, the solvent will evaporate.The grounded collector will attract the charged fiber.The morphology of the electrospun nanofibers were analyzed using Field Emission Scanning Electron Microscopy (FE-SEM) and Transmission Electron Microscopy(TEM).The hydrophilicity of electrospun nanofibers were determined using Surface Contact Angle machine.Fourier Transform Infrared Spectrometry(FT-IR)was used to detect the organic group of the electrospun nanofibers.In vitro release studies were conducted to evaluate sustained release potential of the core-sheath structure composite nanofiber.The results showed that the TEM images clearly proved the core/shell structure of nanofibers for the encapsulation of Drug 0066/Drug 0350 within Polymer A.SEM also showed there was an arch appeared within the nanofiber.The present study would provide a basis for further design and optimization of processing conditions to control the nanostructure of core-sheath composite nanofibers and ultimately achieve desired release kinetics of bioactive proteins(e.g., growth factors)for practical tissue engineering applications.
format	Undergraduates Project Papers
author	Mohamad Muhaimin, Zalani
author_facet	Mohamad Muhaimin, Zalani
author_sort	Mohamad Muhaimin, Zalani
title	Encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning
title_short	Encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning
title_full	Encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning
title_fullStr	Encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning
title_full_unstemmed	Encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning
title_sort	encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/5087/ http://umpir.ump.edu.my/id/eprint/5087/ http://umpir.ump.edu.my/id/eprint/5087/1/CD6489.pdf
first_indexed	2023-09-18T22:00:14Z
last_indexed	2023-09-18T22:00:14Z
_version_	1777414349138165760

Encapsulation of therapeutic protein within polymeric nanofiber using co-axial electrospinning

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