Production and separation of glucose from cellulose hydrolysates using membrane reactor
Sawdust from hardwood contain large amount of cellulose and hemicellulose.Enzymatic hydrolysis of cellulose has larger potential in fulfil global food and energy demand by reducing sugar production such as glucose.In this study,the effect of transmembrane pressure(TMP)and cross-flow velocity(CFV)on...
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| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2012
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| Online Access: | http://umpir.ump.edu.my/id/eprint/4512/ http://umpir.ump.edu.my/id/eprint/4512/ http://umpir.ump.edu.my/id/eprint/4512/1/CD6473_MOHD_HAFIZUDDIN_ZAHARI.pdf |
| Summary: | Sawdust from hardwood contain large amount of cellulose and hemicellulose.Enzymatic hydrolysis of cellulose has larger potential in fulfil global food and energy demand by reducing sugar production such as glucose.In this study,the effect of transmembrane pressure(TMP)and cross-flow velocity(CFV)on permeate flux during the recovery and separation of glucose from cellulose hydrolysates by using membrane reactor was investigated.Two-stage pretreatment will be performed by using dilute sodium hydroxide (NaOH) and follow by dilute sulfuric acid(H2SO)for about 24 hours at 75ºC respectively.Then,continued with enzymatic hydrolysis of cellulose with cellulase and cellobiase for 48 hours at 50 ºC and 150 rpm.Separation of glucose from cellulose hydrolysate will be performed by using ultrafiltration membrane for 60 minutes at 50ºC respectively.Then filtration method using ultrafiltration membrane was employed as a function of transmembrane pressure (TMP) and cross flow velocity (CFV),in order to identify their effects on the membrane flux and subsequently determine its optimum condition using response surface methodology (RSM).Filtration process was conducted at five different values of TMP and CFV range from 1 to 3 bars and 0.06 to 0.22 m/s respectively.The membrane flux after optimization was 116.655 L/m24.h.The optimum conditions at TMP and CFV were found at 1 bar and 0.18 m/s. |
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