Ultrasound-assisted fermentation enhances bioethanol productivity

Production of ethanol from lactose by fermentation with the yeast Kluyveromyces marxianus (ATCC 46537)under various sonication regimens is reported. Batch fermentations were carried out at low-intensity sonication (11.8Wcm−2 sonication intensity at the sonotrode tip) using 10%, 20% and 40% duty cycl...

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Main Authors:	Ahmad Ziad, Sulaiman, Azilah, Ajit, R. M., Yunus, Chisti, Yusuf
Format:	Article
Language:	English
Published:	journal homepage: www.elsevier.com/locate/bej 2011
Subjects:	TA Engineering (General). Civil engineering (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/4501/ http://umpir.ump.edu.my/id/eprint/4501/ http://umpir.ump.edu.my/id/eprint/4501/ http://umpir.ump.edu.my/id/eprint/4501/1/Dr._A.Ziad_SD1.pdf

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recordtype	eprints
spelling	ump-45012018-01-15T03:53:51Z http://umpir.ump.edu.my/id/eprint/4501/ Ultrasound-assisted fermentation enhances bioethanol productivity Ahmad Ziad, Sulaiman Azilah, Ajit R. M., Yunus Chisti, Yusuf TA Engineering (General). Civil engineering (General) Production of ethanol from lactose by fermentation with the yeast Kluyveromyces marxianus (ATCC 46537)under various sonication regimens is reported. Batch fermentations were carried out at low-intensity sonication (11.8Wcm−2 sonication intensity at the sonotrode tip) using 10%, 20% and 40% duty cycles. (A duty cycle of 10%, for example,wasequivalent to sonication for 1 s followed by a rest period (no sonication)of 10 s.) Fermentations were carried out in a 7.5 L (3 L working volume) stirred bioreactor. The sonotrode was mounted in an external chamber and the fermentation broth was continuously recirculated between the bioreactor and the sonication chamber. The flow rate through the sonication loop was 0.2 L min−1.All duty cycles tested improved ethanol production relative to control (no sonication). A 20% duty cycle appeared to be optimal. With this cycle, a final ethanol concentration of 5.20±0.68 g L−1 was obtained, or nearly 3.5-fold that of the control fermentation. Sonication at 10% and 20% cycles appeared to stimulate yeast growth compared to the control fermentation, but 40% duty cycle had a measureable adverse impact on cell growth. Sonication at 10% and 20% cycles enhanced both the extracellular and the intracellular levels of -galactosidase enzyme. Although at the highest duty cycle sonication reduced cell growth, cell viability remained at ≥70% during most of the fermentation. Sonication at a controlled temperature can be used to substantially enhance productivity of bioethanol fermentations journal homepage: www.elsevier.com/locate/bej 2011-02-24 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/4501/1/Dr._A.Ziad_SD1.pdf Ahmad Ziad, Sulaiman and Azilah, Ajit and R. M., Yunus and Chisti, Yusuf (2011) Ultrasound-assisted fermentation enhances bioethanol productivity. Biochemical Engineering Journal. pp. 141-150. http://www.sciencedirect.com/science/article/pii/S1369703X11000209 DOI: 10.1016/j.bej.2011.01.006
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TA Engineering (General). Civil engineering (General)
spellingShingle	TA Engineering (General). Civil engineering (General) Ahmad Ziad, Sulaiman Azilah, Ajit R. M., Yunus Chisti, Yusuf Ultrasound-assisted fermentation enhances bioethanol productivity
description	Production of ethanol from lactose by fermentation with the yeast Kluyveromyces marxianus (ATCC 46537)under various sonication regimens is reported. Batch fermentations were carried out at low-intensity sonication (11.8Wcm−2 sonication intensity at the sonotrode tip) using 10%, 20% and 40% duty cycles. (A duty cycle of 10%, for example,wasequivalent to sonication for 1 s followed by a rest period (no sonication)of 10 s.) Fermentations were carried out in a 7.5 L (3 L working volume) stirred bioreactor. The sonotrode was mounted in an external chamber and the fermentation broth was continuously recirculated between the bioreactor and the sonication chamber. The flow rate through the sonication loop was 0.2 L min−1.All duty cycles tested improved ethanol production relative to control (no sonication). A 20% duty cycle appeared to be optimal. With this cycle, a final ethanol concentration of 5.20±0.68 g L−1 was obtained, or nearly 3.5-fold that of the control fermentation. Sonication at 10% and 20% cycles appeared to stimulate yeast growth compared to the control fermentation, but 40% duty cycle had a measureable adverse impact on cell growth. Sonication at 10% and 20% cycles enhanced both the extracellular and the intracellular levels of -galactosidase enzyme. Although at the highest duty cycle sonication reduced cell growth, cell viability remained at ≥70% during most of the fermentation. Sonication at a controlled temperature can be used to substantially enhance productivity of bioethanol fermentations
format	Article
author	Ahmad Ziad, Sulaiman Azilah, Ajit R. M., Yunus Chisti, Yusuf
author_facet	Ahmad Ziad, Sulaiman Azilah, Ajit R. M., Yunus Chisti, Yusuf
author_sort	Ahmad Ziad, Sulaiman
title	Ultrasound-assisted fermentation enhances bioethanol productivity
title_short	Ultrasound-assisted fermentation enhances bioethanol productivity
title_full	Ultrasound-assisted fermentation enhances bioethanol productivity
title_fullStr	Ultrasound-assisted fermentation enhances bioethanol productivity
title_full_unstemmed	Ultrasound-assisted fermentation enhances bioethanol productivity
title_sort	ultrasound-assisted fermentation enhances bioethanol productivity
publisher	journal homepage: www.elsevier.com/locate/bej
publishDate	2011
url	http://umpir.ump.edu.my/id/eprint/4501/ http://umpir.ump.edu.my/id/eprint/4501/ http://umpir.ump.edu.my/id/eprint/4501/ http://umpir.ump.edu.my/id/eprint/4501/1/Dr._A.Ziad_SD1.pdf
first_indexed	2023-09-18T21:59:09Z
last_indexed	2023-09-18T21:59:09Z
_version_	1777414280682930176

Ultrasound-assisted fermentation enhances bioethanol productivity

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