Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer

Nowadays,with the increase demand of protein production and purity,the purification cost of the protein also increases about 50 to 90%. Packed bed chromatography is widely used in protein separation.However,there are some limitations of the packed bed chromatography such as high-pressure drop, chann...

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Main Author:	Nurul Alia, Khalil
Format:	Undergraduates Project Papers
Language:	English
Published:	2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/4489/ http://umpir.ump.edu.my/id/eprint/4489/ http://umpir.ump.edu.my/id/eprint/4489/1/CD6461NURUL_ALIA_KHALIL_u.pdf

id	ump-4489
recordtype	eprints
spelling	ump-44892015-03-03T09:19:19Z http://umpir.ump.edu.my/id/eprint/4489/ Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer Nurul Alia, Khalil TP Chemical technology Nowadays,with the increase demand of protein production and purity,the purification cost of the protein also increases about 50 to 90%. Packed bed chromatography is widely used in protein separation.However,there are some limitations of the packed bed chromatography such as high-pressure drop, channeling problem and complicated scale up process.Most of these limitations can be overcome by membrane chromatography.The main objective of this research is to prepare cation-exchange membrane chromatography from polyamide membrane by chemical grafting of methacrylic acid monomer.Potassium persulfate and potassium metabisulfite was used to generate the radicals in the polyamide membrane and grafted with methacrylic acid monomer together with ethylene glycol dimethacrylate as a cross-linker.Different monomer concentration between 0.1 and 1.0M and monomer grafting duration from 15 – 120 min was studied toward producing high protein binding capacity membrane.Optimum time for grafting methacrylic acid onto polyamide membrane was 45 minutes with the binding capacity 599.6390 mg/g BSA at the monomer concentration of 0.1 M.Identification and optimization of critical parameter in grafting process is crucial for the development of high performance membrane chromatography materials. 2012-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/4489/1/CD6461NURUL_ALIA_KHALIL_u.pdf Nurul Alia, Khalil (2012) Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer. Faculty of Chemical & Natural Resource Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:68064&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nurul Alia, Khalil Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer
description	Nowadays,with the increase demand of protein production and purity,the purification cost of the protein also increases about 50 to 90%. Packed bed chromatography is widely used in protein separation.However,there are some limitations of the packed bed chromatography such as high-pressure drop, channeling problem and complicated scale up process.Most of these limitations can be overcome by membrane chromatography.The main objective of this research is to prepare cation-exchange membrane chromatography from polyamide membrane by chemical grafting of methacrylic acid monomer.Potassium persulfate and potassium metabisulfite was used to generate the radicals in the polyamide membrane and grafted with methacrylic acid monomer together with ethylene glycol dimethacrylate as a cross-linker.Different monomer concentration between 0.1 and 1.0M and monomer grafting duration from 15 – 120 min was studied toward producing high protein binding capacity membrane.Optimum time for grafting methacrylic acid onto polyamide membrane was 45 minutes with the binding capacity 599.6390 mg/g BSA at the monomer concentration of 0.1 M.Identification and optimization of critical parameter in grafting process is crucial for the development of high performance membrane chromatography materials.
format	Undergraduates Project Papers
author	Nurul Alia, Khalil
author_facet	Nurul Alia, Khalil
author_sort	Nurul Alia, Khalil
title	Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer
title_short	Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer
title_full	Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer
title_fullStr	Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer
title_full_unstemmed	Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer
title_sort	preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/4489/ http://umpir.ump.edu.my/id/eprint/4489/ http://umpir.ump.edu.my/id/eprint/4489/1/CD6461NURUL_ALIA_KHALIL_u.pdf
first_indexed	2023-09-18T21:59:07Z
last_indexed	2023-09-18T21:59:07Z
_version_	1777414279538933760

Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer

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