Purification of fructosyltransferase (FTase) from Aspergillus Niger to enhance production of Fructooligosaccharides (FOS) as a food additive

Purification of fructosyltransferase (FTase) from Aspergillus Niger to enhance production of Fructooligosaccharides (FOS) as a food additive

Nowadays,worldwide consumers are becoming increasingly aware of the relationship between food or food constituents and health.In response to an increasing demand from the consumer,fructooligosaccharides (FOS) have emerged primarily because of its functional properties rather than sweeteners.The enzy...

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Main Author: Muhamad Aliff, Ramli
Format: Undergraduates Project Papers
Language:English
Published: 2012
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/4388/
http://umpir.ump.edu.my/id/eprint/4388/
http://umpir.ump.edu.my/id/eprint/4388/1/CD6362_MUHAMAD_ALIFF_RAMLI.pdf
id ump-4388
recordtype eprints
spelling ump-43882015-03-03T09:18:46Z http://umpir.ump.edu.my/id/eprint/4388/ Purification of fructosyltransferase (FTase) from Aspergillus Niger to enhance production of Fructooligosaccharides (FOS) as a food additive Muhamad Aliff, Ramli TP Chemical technology Nowadays,worldwide consumers are becoming increasingly aware of the relationship between food or food constituents and health.In response to an increasing demand from the consumer,fructooligosaccharides (FOS) have emerged primarily because of its functional properties rather than sweeteners.The enzyme source synthesis can be divided into two classes which are from plant and microorganism. Microorganism producing FOS from FTase had captured much attention from industrial level due to mass production and controlled environment rather than FOS produced from plant. Many researchers produced crude FTase enzyme to produce FOS. In view of that,purification of crude enzyme from microfungi is studied in order to obtain high yield of FOS.This study is carried out using molasses as a substrate in the fermentation process to produce crude enzyme from microorganism.The series of purification step will be done in order to purify the enzyme and to determine the characteristic of purified enzyme. By using an extracellular enzyme which is fructosyltransferase (FTase) from selected micro fungi,the FOS can be produced commercially from sucrose.The enzyme was able to transfer the fructosyl group from sucrose as donor producing corresponding series of FOS: 1-kestose,nystose and fructosylnystose.Although these proteins differ in their subunit structure,molecular weight,chemical susceptibility and substrate specificity, they all display both hydrolytic and transfer activities which limit the FOS production to the use of high sucrose concentration.The optimum pH and temperature for activity of FTase is between 5 to 6.5 and 50 °C to 60 °C with yield of 125 U/mL when crude FTase is used and 136.7 U/mL when purified FTase is used.For the purification studies of FTase, different micro fungi will produce different characteristic of enzyme.The enzyme was able to transfer fructosyl groups from sucrose and then catalyze the formation of short chain FOS. 2012-02 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/4388/1/CD6362_MUHAMAD_ALIFF_RAMLI.pdf Muhamad Aliff, Ramli (2012) Purification of fructosyltransferase (FTase) from Aspergillus Niger to enhance production of Fructooligosaccharides (FOS) as a food additive. Faculty of Chemical & Natural Resource Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:67369&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Muhamad Aliff, Ramli
Purification of fructosyltransferase (FTase) from Aspergillus Niger to enhance production of Fructooligosaccharides (FOS) as a food additive
description Nowadays,worldwide consumers are becoming increasingly aware of the relationship between food or food constituents and health.In response to an increasing demand from the consumer,fructooligosaccharides (FOS) have emerged primarily because of its functional properties rather than sweeteners.The enzyme source synthesis can be divided into two classes which are from plant and microorganism. Microorganism producing FOS from FTase had captured much attention from industrial level due to mass production and controlled environment rather than FOS produced from plant. Many researchers produced crude FTase enzyme to produce FOS. In view of that,purification of crude enzyme from microfungi is studied in order to obtain high yield of FOS.This study is carried out using molasses as a substrate in the fermentation process to produce crude enzyme from microorganism.The series of purification step will be done in order to purify the enzyme and to determine the characteristic of purified enzyme. By using an extracellular enzyme which is fructosyltransferase (FTase) from selected micro fungi,the FOS can be produced commercially from sucrose.The enzyme was able to transfer the fructosyl group from sucrose as donor producing corresponding series of FOS: 1-kestose,nystose and fructosylnystose.Although these proteins differ in their subunit structure,molecular weight,chemical susceptibility and substrate specificity, they all display both hydrolytic and transfer activities which limit the FOS production to the use of high sucrose concentration.The optimum pH and temperature for activity of FTase is between 5 to 6.5 and 50 °C to 60 °C with yield of 125 U/mL when crude FTase is used and 136.7 U/mL when purified FTase is used.For the purification studies of FTase, different micro fungi will produce different characteristic of enzyme.The enzyme was able to transfer fructosyl groups from sucrose and then catalyze the formation of short chain FOS.
format Undergraduates Project Papers
author Muhamad Aliff, Ramli
author_facet Muhamad Aliff, Ramli
author_sort Muhamad Aliff, Ramli
title Purification of fructosyltransferase (FTase) from Aspergillus Niger to enhance production of Fructooligosaccharides (FOS) as a food additive
title_short Purification of fructosyltransferase (FTase) from Aspergillus Niger to enhance production of Fructooligosaccharides (FOS) as a food additive
title_full Purification of fructosyltransferase (FTase) from Aspergillus Niger to enhance production of Fructooligosaccharides (FOS) as a food additive
title_fullStr Purification of fructosyltransferase (FTase) from Aspergillus Niger to enhance production of Fructooligosaccharides (FOS) as a food additive
title_full_unstemmed Purification of fructosyltransferase (FTase) from Aspergillus Niger to enhance production of Fructooligosaccharides (FOS) as a food additive
title_sort purification of fructosyltransferase (ftase) from aspergillus niger to enhance production of fructooligosaccharides (fos) as a food additive
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/4388/
http://umpir.ump.edu.my/id/eprint/4388/
http://umpir.ump.edu.my/id/eprint/4388/1/CD6362_MUHAMAD_ALIFF_RAMLI.pdf
first_indexed 2023-09-18T21:58:55Z
last_indexed 2023-09-18T21:58:55Z
_version_ 1777414266435928064

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