Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)

Factors influencing poly(3-hydroxybutyrate) P(3HB) production by Cupriavidus necator CCUG52238T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigate...

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Main Authors:	Mior Ahmad Khushairi, Mohd Zahari, Jailani, Salihon
Format:	Article
Language:	English
Published:	Hindawi Publishing Corporation 2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3784/ http://umpir.ump.edu.my/id/eprint/3784/ http://umpir.ump.edu.my/id/eprint/3784/1/2012_Mohd_Zahari_et_al_Factors_Affecting.pdf

id	ump-3784
recordtype	eprints
spelling	ump-37842018-02-19T06:43:05Z http://umpir.ump.edu.my/id/eprint/3784/ Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T) Mior Ahmad Khushairi, Mohd Zahari Jailani, Salihon TP Chemical technology Factors influencing poly(3-hydroxybutyrate) P(3HB) production by Cupriavidus necator CCUG52238T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH4)2SO4 concentration at 0.5 g/L. By culturing the wild-type strain of C. necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ± 0.13 g/L and 45 ± 1.5 wt.%, respectively. This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition. In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor. Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%. P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3784/1/2012_Mohd_Zahari_et_al_Factors_Affecting.pdf Mior Ahmad Khushairi, Mohd Zahari and Jailani, Salihon (2012) Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T). Journal of Biomedicine and Biotechnology, 2012. http://www.hindawi.com/journals/bmri/2012/125865/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mior Ahmad Khushairi, Mohd Zahari Jailani, Salihon Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
description	Factors influencing poly(3-hydroxybutyrate) P(3HB) production by Cupriavidus necator CCUG52238T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH4)2SO4 concentration at 0.5 g/L. By culturing the wild-type strain of C. necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ± 0.13 g/L and 45 ± 1.5 wt.%, respectively. This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition. In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor. Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%. P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate.
format	Article
author	Mior Ahmad Khushairi, Mohd Zahari Jailani, Salihon
author_facet	Mior Ahmad Khushairi, Mohd Zahari Jailani, Salihon
author_sort	Mior Ahmad Khushairi, Mohd Zahari
title	Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
title_short	Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
title_full	Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
title_fullStr	Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
title_full_unstemmed	Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
title_sort	factors affecting poly(3-hydroxybutyrate) product ion from oil palm frond juice by cupriavidus necator (ccug52238t)
publisher	Hindawi Publishing Corporation
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/3784/ http://umpir.ump.edu.my/id/eprint/3784/ http://umpir.ump.edu.my/id/eprint/3784/1/2012_Mohd_Zahari_et_al_Factors_Affecting.pdf
first_indexed	2023-09-18T21:58:17Z
last_indexed	2023-09-18T21:58:17Z
_version_	1777414227095453696

Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)

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