Avicelase Enzyme from Sawdust: Isolation, Production and Optimization

Economical disposal of sawdust is a problem of growing concern to the wood industries as waste. On an industry-wide basis most of sawdust is green and has many uses that able to be converted to useful products such as fuel, chemical feedstock or textiles. Cellulose is a potential substrate to the en...

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Main Authors:	Makky, Essam A., Noor Afifah, Fauzi
Format:	Article
Language:	English English English
Published:	www.mutagens.co.in 2014
Subjects:	QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/3711/ http://umpir.ump.edu.my/id/eprint/3711/ http://umpir.ump.edu.my/id/eprint/3711/1/Makky_Paper.pdf http://umpir.ump.edu.my/id/eprint/3711/2/fist-2014-makky-AvicelaseEnzyme.pdf http://umpir.ump.edu.my/id/eprint/3711/7/Avicelase%20Enzyme%20From%20Sawdust-%20Isolation%2C%20Production%20And%20Optimization.pdf

id	ump-3711
recordtype	eprints
spelling	ump-37112017-08-09T06:55:08Z http://umpir.ump.edu.my/id/eprint/3711/ Avicelase Enzyme from Sawdust: Isolation, Production and Optimization Makky, Essam A. Noor Afifah, Fauzi QR Microbiology Economical disposal of sawdust is a problem of growing concern to the wood industries as waste. On an industry-wide basis most of sawdust is green and has many uses that able to be converted to useful products such as fuel, chemical feedstock or textiles. Cellulose is a potential substrate to the enzyme that can be produced from the activation of cellulose conversion into monomer. Using microorganisms such as bacteria and fungi as a converter, cellulose can be degraded and converted into reducing sugar with the help of various enzymes including Avicelase enzyme. The utilization of sawdust as a potential substrate for producing enzymes may serve a dual purpose of reducing the environmental pollution along with producing a high value commercial product. Effect of nutritional parameters such as initial pH, carbon and nitrogen sources for Avicelase production from Bacillus sp. was investigated. From three different sources of isolates, only one of the most potent isolate was selected from a food source CL5 which has been partially identified and suggested to be Bacillus species. Optimum pH-value, carbon and nitrogen sources for Avicelase production found to be 7.0, lactose and KNO3 were (2.450±0.009 IU/ml) increased up to 3556.7% with sp. act. 1.528 IU/mg; (1.286±0.074 IU/ml) increased up to 1548.7% with sp. act. 0.631 IU/mg and (2.287±0.109 IU/ml) increased to 3313.4% with sp. act. 0.979 IU/mg, respectively www.mutagens.co.in 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3711/1/Makky_Paper.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/3711/2/fist-2014-makky-AvicelaseEnzyme.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/3711/7/Avicelase%20Enzyme%20From%20Sawdust-%20Isolation%2C%20Production%20And%20Optimization.pdf Makky, Essam A. and Noor Afifah, Fauzi (2014) Avicelase Enzyme from Sawdust: Isolation, Production and Optimization. Journal of Global Biosciences, 3 (1). pp. 299-303. ISSN 2320-1355 http://www.mutagens.co.in/jgb/vol.03/1/07.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English English
topic	QR Microbiology
spellingShingle	QR Microbiology Makky, Essam A. Noor Afifah, Fauzi Avicelase Enzyme from Sawdust: Isolation, Production and Optimization
description	Economical disposal of sawdust is a problem of growing concern to the wood industries as waste. On an industry-wide basis most of sawdust is green and has many uses that able to be converted to useful products such as fuel, chemical feedstock or textiles. Cellulose is a potential substrate to the enzyme that can be produced from the activation of cellulose conversion into monomer. Using microorganisms such as bacteria and fungi as a converter, cellulose can be degraded and converted into reducing sugar with the help of various enzymes including Avicelase enzyme. The utilization of sawdust as a potential substrate for producing enzymes may serve a dual purpose of reducing the environmental pollution along with producing a high value commercial product. Effect of nutritional parameters such as initial pH, carbon and nitrogen sources for Avicelase production from Bacillus sp. was investigated. From three different sources of isolates, only one of the most potent isolate was selected from a food source CL5 which has been partially identified and suggested to be Bacillus species. Optimum pH-value, carbon and nitrogen sources for Avicelase production found to be 7.0, lactose and KNO3 were (2.450±0.009 IU/ml) increased up to 3556.7% with sp. act. 1.528 IU/mg; (1.286±0.074 IU/ml) increased up to 1548.7% with sp. act. 0.631 IU/mg and (2.287±0.109 IU/ml) increased to 3313.4% with sp. act. 0.979 IU/mg, respectively
format	Article
author	Makky, Essam A. Noor Afifah, Fauzi
author_facet	Makky, Essam A. Noor Afifah, Fauzi
author_sort	Makky, Essam A.
title	Avicelase Enzyme from Sawdust: Isolation, Production and Optimization
title_short	Avicelase Enzyme from Sawdust: Isolation, Production and Optimization
title_full	Avicelase Enzyme from Sawdust: Isolation, Production and Optimization
title_fullStr	Avicelase Enzyme from Sawdust: Isolation, Production and Optimization
title_full_unstemmed	Avicelase Enzyme from Sawdust: Isolation, Production and Optimization
title_sort	avicelase enzyme from sawdust: isolation, production and optimization
publisher	www.mutagens.co.in
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/3711/ http://umpir.ump.edu.my/id/eprint/3711/ http://umpir.ump.edu.my/id/eprint/3711/1/Makky_Paper.pdf http://umpir.ump.edu.my/id/eprint/3711/2/fist-2014-makky-AvicelaseEnzyme.pdf http://umpir.ump.edu.my/id/eprint/3711/7/Avicelase%20Enzyme%20From%20Sawdust-%20Isolation%2C%20Production%20And%20Optimization.pdf
first_indexed	2023-09-18T21:58:09Z
last_indexed	2023-09-18T21:58:09Z
_version_	1777414218698457088

Avicelase Enzyme from Sawdust: Isolation, Production and Optimization

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