Mixed-mode interaction membrane chromatography for protein fractionation

Mixed-mode interaction membrane chromatography for protein fractionation

Nowadays, membrane chromatography has widely use in protein separation. Most of the membrane chromatography is prepared through a chemical modification which sometimes can changes the structure and properties of the membrane itself. In this study, membrane chromatography was prepared through physica...

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Main Author: Nur Farahain, Khusnun
Format: Undergraduates Project Papers
Language:English
Published: 2012
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/3607/
http://umpir.ump.edu.my/id/eprint/3607/
http://umpir.ump.edu.my/id/eprint/3607/1/CD6339_NURFARAHAIN_KHUSNUN.pdf
id ump-3607
recordtype eprints
spelling ump-36072015-03-03T08:02:07Z http://umpir.ump.edu.my/id/eprint/3607/ Mixed-mode interaction membrane chromatography for protein fractionation Nur Farahain, Khusnun TP Chemical technology Nowadays, membrane chromatography has widely use in protein separation. Most of the membrane chromatography is prepared through a chemical modification which sometimes can changes the structure and properties of the membrane itself. In this study, membrane chromatography was prepared through physical modification using the mixed matrix membrane preparation concept. Amberlite IR120 cation exchange resin (CEX) and Lewatit MP500 anion exchange resin (AEX) was used to formulate mixed mode interaction membrane chromatography (MMIMC) based on ethylene vinyl alcohol base membrane matrix. The total resin loading from 20-50% and the ratio between anion exchange resin and cation exchange resin was studied. This research found the optimum resin loading is 30%. Pure AEX MMM has the binding capacity of 340.26 mg BSA/g membrane and pure CEX MMM has a binding capacity of 3166.22 mg LZY/g membrane. The MMIMC with ratio CEX/AEX equal to 50/50 has a capacity of 895.898 mg LZY/g membrane and 3430.74 mg BSA/g membrane. A high performance MMIMC can be produced by optimization the loading percentage of ion exchanger resin and the ratio of CEX/AEX resin incorporate into the membrane. 2012-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3607/1/CD6339_NURFARAHAIN_KHUSNUN.pdf Nur Farahain, Khusnun (2012) Mixed-mode interaction membrane chromatography for protein fractionation. Faculty of Chemical Engineering and Natural Resources, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:67228&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nur Farahain, Khusnun
Mixed-mode interaction membrane chromatography for protein fractionation
description Nowadays, membrane chromatography has widely use in protein separation. Most of the membrane chromatography is prepared through a chemical modification which sometimes can changes the structure and properties of the membrane itself. In this study, membrane chromatography was prepared through physical modification using the mixed matrix membrane preparation concept. Amberlite IR120 cation exchange resin (CEX) and Lewatit MP500 anion exchange resin (AEX) was used to formulate mixed mode interaction membrane chromatography (MMIMC) based on ethylene vinyl alcohol base membrane matrix. The total resin loading from 20-50% and the ratio between anion exchange resin and cation exchange resin was studied. This research found the optimum resin loading is 30%. Pure AEX MMM has the binding capacity of 340.26 mg BSA/g membrane and pure CEX MMM has a binding capacity of 3166.22 mg LZY/g membrane. The MMIMC with ratio CEX/AEX equal to 50/50 has a capacity of 895.898 mg LZY/g membrane and 3430.74 mg BSA/g membrane. A high performance MMIMC can be produced by optimization the loading percentage of ion exchanger resin and the ratio of CEX/AEX resin incorporate into the membrane.
format Undergraduates Project Papers
author Nur Farahain, Khusnun
author_facet Nur Farahain, Khusnun
author_sort Nur Farahain, Khusnun
title Mixed-mode interaction membrane chromatography for protein fractionation
title_short Mixed-mode interaction membrane chromatography for protein fractionation
title_full Mixed-mode interaction membrane chromatography for protein fractionation
title_fullStr Mixed-mode interaction membrane chromatography for protein fractionation
title_full_unstemmed Mixed-mode interaction membrane chromatography for protein fractionation
title_sort mixed-mode interaction membrane chromatography for protein fractionation
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/3607/
http://umpir.ump.edu.my/id/eprint/3607/
http://umpir.ump.edu.my/id/eprint/3607/1/CD6339_NURFARAHAIN_KHUSNUN.pdf
first_indexed 2023-09-18T21:58:00Z
last_indexed 2023-09-18T21:58:00Z
_version_ 1777414208269320192

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